Antimicrobial and cytotoxic activities of some crude drug extracts from Mexican medicinal plants

Gutiérrez-Lugo, Maria Teresa; Barrientos-Benítez, T.; Cervantes-Luna, Brenda Sarahi; Ramírez-Gama, Rosa María; Bye, Robert; Linares, Edelmira; Mata, Rachel

doi:10.1016/s0944-7113(96)80079-1

Cited by 27 publications

(22 citation statements)

References 22 publications

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“…Other authors have conducted studies using a similar working scheme with several possible antimicrobial substances; however, none used silver nitrate [20,21,23]. Indeed, the majority of studies refer either to antimicrobial or to cytotoxic activity.…”

Section: Discussionmentioning

confidence: 99%

Silver Nitrate: Antimicrobial Activity Related to Cytotoxicity in Cultured Human Fibroblasts

Hidalgo

Bartolomé²,

Barroso³

et al. 1998

Skin Pharmacol Physiol

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The aims of this study were to ascertain whether silver nitrate (AgNO₃) concentrations below those used in clinical practice inhibit bacterial growth, and in parallel study the cytotoxic effects on human fibroblasts. The cytoprotective effects of fetal calf serum (FCS) were also evaluated. The cytotoxic effects of eight different silver nitrate concentrations were determined by assessing mitochondrial activity of viable cells capable of cleaving tetrazolium salts. Antimicrobial activity of AgNO₃, range: 7–550 × 10^–5%, was tested against Staphylococcus aureus, Citrobacter freundii, and Pseudomonas aeruginosa. Silver nitrate concentrations exerting antimicrobial effects were: S. aureus, >70 × 10^–5%; P. aeruginosa, ≥270 × 10^–5%, and C. freundii, ≥550 × 10^–5%. With 2% FCS, the lowest AgNO₃ concentration studied (7 × 10^–5%) showed cytotoxic effects (cell survival 71 ± 19%) at only 2 h of incubation. Under these conditions AgNO₃ cytotoxicity was time- and concentration-dependent in all exposure periods. Cytotoxicity was greatly enhanced causing 76% fibroblast growth inhibition at concenctrations of 14 × 10^–5% and contact time of 2 h. The AgNO₃ concentration of 7 × 10^–5% was also cytotoxic with 5% FCS in the media compared with controls, although cell survival was higher than with 2% FCS. The cytoprotective action of FCS was clearly shown at the concentration of 10% at which AgNO₃ cytotoxicity of 7 × 10^–5% to 28 × 10^–5% was partially or completely inhibited. Our results show that AgNO₃ at concentrations 100–700 times more diluted than that normally used in clinical practice retained effective inhibitory activity against some of the above-mentioned microorganisms. However, even these concentrations are cytotoxic for cultured fibroblasts. Thus, silver nitrate concentrations up to 100 times more diluted can be used, since they possess bacterial growth-inhibiting power, are less cytotoxic and therefore favour wound healing.

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Section: Discussionmentioning

confidence: 99%

Silver Nitrate: Antimicrobial Activity Related to Cytotoxicity in Cultured Human Fibroblasts

Hidalgo

Bartolomé²,

Barroso³

et al. 1998

Skin Pharmacol Physiol

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“…La afinina es la alcamida mayoritaria en las raíces de esta planta y principal responsable de los efectos biológicos específicos observados, entre los que se pueden considerar la acción de anestésico local, el estímulo organoléptico, así como la actividad insecticida y bactericida. Por mucho tiempo se consideró a la afinina como único componente bioactivo en este tejido, cuyo extracto crudo se ha utilizado para el estudio de sus aplicaciones, mismas que recientemente se han extendido (Romero et al, 1989;Gutiérrez-Lugo et al, 1996) mostrando un potencial agronómico importante en el control biológico de bacterias y hongos fitopatógenos (Ramírez-Chávez et al, 2000). El análisis fitoquímico más detallado de las raíces de H. longipes ha revelado la presencia de otros principios bioactivos y de propiedades antimicrobianas de algunas alcamidas individuales (Molina-Torres et al, 1995,1999.…”

Section: Aspectos Químicos Alcamidas En El Género Heliopsisunclassified

El género Heliopsis (Heliantheae; Asteraceae) en México y las alcamidas presentes en sus raíces

García¹,

Ramírez²,

Molina³

2004

Acta Bot. Mex.

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-IPN Unidad Irapuato, km 9.6 Libramiento Norte, Apdo. postal 629, 36500 Irapuato, Guanajuato, México RESUMENEl género Heliopsis pertenece a la tribu Heliantheae de la familia Asteraceae. Incluye 14 especies, la mayoría endémicas a México y aún no todas totalmente definidas. Una de ellas, Heliopsis longipes, utilizada en la medicina tradicional, se ha caracterizado por el alto contenido de alcamidas en sus raíces. El estudio de tales metabolitos ha derivado en una serie de investigaciones sobre su presencia en otros representantes del género Heliopsis de México. El escrito sintetiza y actualiza la información bibliográfica referente a la taxonomía del género y se presentan los resultados obtenidos del análisis cuantitativo y cualitativo de las alcamidas detectadas en raíces de seis especies. Se discute la importancia quimiotaxonómica de estos metabolitos en el mismo género.Palabras clave: alcamidas, Asteraceae, Heliantheae, Heliopsis, México. ABSTRACTThe genus Heliopsis belongs to the tribe Heliantheae in the Asteraceae family. It includes 14 species, most of them endemic to Mexico. One of the species, Heliopsis longipes, is utilized in traditional medicine. The high level of alkamides in its roots has already been chemically characterized. The study of these metabolites is displayed in a comparative analysis on the presence of alkamides in six Mexican species of Heliopsis. The quantitative and qualitative analysis of the alkamides stored in roots of each species is presented. Chemotaxonomic importance of alkamides in this genus is also discussed. In this work we review and update the bibliography referring to the taxonomy of the genus.Key words: alkamides, Asteraceae, Heliantheae, Heliopsis, Mexico. 69: 115-131 (2004) Acta Botanica Mexicana ASPECTOS BOTÁNICOS Datos generales relativos a HeliopsisEl género Heliopsis pertenece a la tribu Heliantheae de la familia Asteraceae. La mencionada tribu comprende cerca de 2,500 especies agrupadas en 189 géneros y está fraccionada en diez subtribus. Heliopsis, asociado Zinnia, Philactis, Sanvitalia, Acmella, Podachaenium, Squamopappus, Spilanthes y Salmea, conforma la subtribu Zinniinae (Bremer, 1994), cuyos miembros comparten, en su morfología, la presencia de capítulos solitarios, receptáculos cónicos o hemisféricos, flores liguladas pistiladas, fértiles y persistentes, flores del disco fértiles y aquenios del disco lateralmente comprimidos o angulados. La mayor diferencia que separa las especies de Heliopsis de las de otros géneros es la siguiente combinación de caracteres: la presencia de flores fértiles en el disco, flores liguladas persistentes y fértiles, corolas sésiles y persistentes, aquenios gruesos, 3 a 4-angulares y vilano ausente.Aunque restringida al hemisferio occidental, la distribución de este género es amplia, abarcando desde la región de las grandes planicies y el oriente de Estados Unidos, hasta Bolivia. Los hábitats donde viven sus especies son variados, desde regiones áridas hasta las francamente húmedas y desde las cálidas hasta las de clima temp...

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“…Los usos tradicionales de la raíz de chilcuague requieren la destrucción total de la planta, lo que ha provocado la reducción de sus poblaciones, o su desaparición en algunas zonas (Cilia et al, 2008;Little, 1948). Los estudios químicos (Acree, Jacobson, & Haller, 1945;Molina-Torres, Salgado-Garciglia, Ramírez-Chávez, & del Río, 1996) y farmacológicos con la raíz de H. longipes han demostrado sus propiedades como bactericida (Gutiérrez-Lugo et al, 1996; Molina-Torres, García-Chávez, & Ramírez-Chávez, 1999) y quadrangular, striated, brown to blackish, pappus absent or only rarely two to four small and membranous awns (Cilia, Reyes, Aguirre, & Juárez, 2013).…”

Section: Introductionunclassified

“…The traditional uses of chilcuague root require the destruction of the entire plant, which has caused reduction in its populations, including total disappearance in some zones (Cilia et al, 2008;Little, 1948). Chemical (Acree, Jacobson, & Haller, 1945;Molina-Torres, Salgado-Garciglia, Ramírez-Chávez, & del Río, 1996) and pharmacological studies with the root of H. longipes have demonstrated its properties as bactericide (Gutiérrez-Lugo et al, 1996; Molina-Torres, García-Chávez, & Ramírez-Chávez, 1999) and fungicide (Ramírez-Chávez, Lucas-Valdez, Virgen-Calleros, & Molina-Torres, 2000). An analgesic effect and stimulants to the central nervous system have also been found (Cilia et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

DISTRIBUCIÓN DE Heliopsis longipes (Heliantheae: Asteraceae), UN RECURSO ENDÉMICO DEL CENTRO DE MÉXICO

Cilia-López¹,

Aguirre‐Rivera²,

Espinosa-Reyes³

et al. 2014

Rev Cha Se Cie For y del Amb

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Heliopsis longipes is a perennial herb endemic to Sierra de Álvarez and Sierra Gorda in central México. It is a species of major economic importance within its genus, due to the traditional uses of its root. Chemical and pharmacological studies have been performed with H. longipes roots, however recent studies regarding its distribution are lacking. The objective of the current study was to establish and document the geographic distribution and existing ecologic conditions of H. longipes. A review of published works regarding this species, as well as an examination of herbarium specimens has been conducted. Explorations in Sierra de Álvarez and Sierra Gorda were also conducted to locate populations of H. longipes. In each site, the physical and biological factors associated with the population were recorded. Heliopsis. longipes is distributed in the states of San Luis Potosí, Guanajuato and Querétaro, in steep canyons with oak and oak-pine forests and substrates of igneous origin, in Sierra de Álvarez and Sierra Gorda.

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Antimicrobial and cytotoxic activities of some crude drug extracts from Mexican medicinal plants

Cited by 27 publications

References 22 publications

Silver Nitrate: Antimicrobial Activity Related to Cytotoxicity in Cultured Human Fibroblasts

Silver Nitrate: Antimicrobial Activity Related to Cytotoxicity in Cultured Human Fibroblasts

El género Heliopsis (Heliantheae; Asteraceae) en México y las alcamidas presentes en sus raíces

DISTRIBUCIÓN DE Heliopsis longipes (Heliantheae: Asteraceae), UN RECURSO ENDÉMICO DEL CENTRO DE MÉXICO

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