Antimicrobial and biofilm-disrupting nanostructured TiO2 coating demonstrating photoactivity and dark activity

Wasa, Alibe; Land, Johann G.; Gorthy, Rukmini; Krumdieck, Susan; Bishop, Courtney; Godsoe, William; Heinemann, Jack A.

doi:10.1093/femsle/fnab039

Cited by 9 publications

(9 citation statements)

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“…Stainless steel (25 × 25 mm) coupons were used as an inert control material as recommended by the ISO 27447:2009 (Mills et al 2012), and commercially pure copper (25 × 25 mm) coupons were selected as a positive control material because they have known antimicrobial properties that are independent of light (Wasa et al 2021). The NsARC (25 × 25 mm) coupons and control (stainless steel and copper) samples were sterilised by immersion in 70% ethanol and the NsARC test pieces were then aseptically stored in the dark for > 48 h. Gelatine coated slides were made by immersing glass microscopy slides (Mareinfeld-Superior, 76 × 26 mm, approx.…”

Section: Bacterial Strains Culture Conditions Materials and Chemicalsmentioning

confidence: 99%

“…The E. coli culture was exposed to the tests and control metal samples using a modified version of ISO 27447:2009 (Wasa et al 2021). 100 µl of the test organisms containing ~ 1,000,000 cells at the stationary growth phase were placed on 25 × 25 mm NsARC and stainlesssteel surfaces and 24 × 24 mm sterile coverslip (Lab Supply, Dunedin (New Zealand)) was place on top, causing the liquid to spread evenly on the surface.…”

Section: Determining If Nsarc Can Cause a Change In Susceptibility Of...mentioning

confidence: 99%

“…Nanostructured anatase rutile and carbon (NsARC), a composite of titania and carbon is an antimicrobial surface coating that is active with or without photoactivation (Wasa et al 2021). The killing mechanism of NsARC has not been precisely described but at least involves free radicals that are released sequel to photoexcitation of TiO 2 (Krumdieck et al 2019), active carbon-centred free radicals (Fenoglio et al 2009) and desiccation of cells over time (Yu et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Should NsARC be used on a commercial scale for its antimicrobial properties, we wanted to know if there were unintended outcomes that we could anticipate. We previously hypothesized that sub-lethal exposures to NsARC could cause similar changes to antibiotic susceptibility (Wasa et al 2021). This is based on the innate response of bacteria to toxic environments.…”

Section: Introductionmentioning

confidence: 99%

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Copper and nanostructured anatase rutile and carbon coatings induce adaptive antibiotic resistance

et al. 2022

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Contaminated surfaces are vehicles for the spread of infectious disease-causing microorganisms. A strategy to prevent their spread is applying antimicrobial coatings to surfaces. Both nanostructured anatase rutile and carbon (NsARC), a TiO2 formulation, and copper are examples of antimicrobial agents that are used in making or coating door handles and similar surfaces, to reduce microbial loads. Antimicrobial surfaces have been extensively tested for antimicrobial activity but not sublethal effects, such as exposure-associated multiple antibiotic resistance phenotypes usually caused by induction of efflux pump genes. The possibility of NsARC and copper inducing indicative efflux pump pathways was investigated by monitoring the expression of mScarlet fluorescent protein (FP) in two reporter strains of Escherichia coli. There was an increase in the expression of FP in the reporter strains exposed to NsARC and copper relative to the inert control composed of stainless steel. Furthermore we tested E. coli and Staphylococcus aureus following 8 h of exposure to NsARC for changes in resistance to selected antibiotics. E. coli that were exposed to NsARC became more susceptible to kanamycin but there was no significant change in susceptibility of S. aureus to any tested antibiotics. These findings suggests that even though NsARC and copper are antimicrobial, they also have some potential to cause unintended phenotypes.

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Section: Bacterial Strains Culture Conditions Materials and Chemicalsmentioning

confidence: 99%

Section: Determining If Nsarc Can Cause a Change In Susceptibility Of...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Copper and nanostructured anatase rutile and carbon coatings induce adaptive antibiotic resistance

et al. 2022

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“…Evaluation of further in vivo root canal infection models from Beagle dog premolars demonstrated excellent antibacterial activities of Au@Cu 2 –X S nanomaterial as novel agents with potential clinical applications in root canal therapy ( Cao et al, 2021 ). A photo-illuminated nanostructured TiO 2 based thin film (NsARC) was used to grow E. coli , S. aureus , Saccharomyces cerevisiae , and P. aeruginosa showed antimicrobial activity and biofilm disruption resulting in efficient photocatalytic activity ( Wasa et al, 2021 ). Similar results were observed in cerium oxide nanoparticles doped with tin (Sn-doped CeO 2 ) synthesized from Allophylus cobbe exhibiting concentration-dependent antibiofilm activity under visible light in Listeria monocytogenes and S. aureus at 512 mg/mL with a 2 log CFU reduction ( Naidi et al, 2021 ).…”

Section: Targeting Major Drug Resistance Determinants In Bacterial Pathogens With Photothermally Active Nanomaterialsmentioning

confidence: 99%

Combating Drug-Resistant Bacteria Using Photothermally Active Nanomaterials: A Perspective Review

et al. 2021

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Injudicious use of antibiotics has been the main driver of severe bacterial non-susceptibility to commonly available antibiotics (known as drug resistance or antimicrobial resistance), a global threat to human health and healthcare. There is an increase in the incidence and levels of resistance to antibacterial drugs not only in nosocomial settings but also in community ones. The drying pipeline of new and effective antibiotics has further worsened the situation and is leading to a potentially “post-antibiotic era.” This requires novel and effective therapies and therapeutic agents for combating drug-resistant pathogenic microbes. Nanomaterials are emerging as potent antimicrobial agents with both bactericidal and potentiating effects reported against drug-resistant microbes. Among them, the photothermally active nanomaterials (PANs) are gaining attention for their broad-spectrum antibacterial potencies driven mainly by the photothermal effect, which is characterized by the conversion of absorbed photon energy into heat energy by the PANs. The current review capitalizes on the importance of using PANs as an effective approach for overcoming bacterial resistance to drugs. Various PANs leveraging broad-spectrum therapeutic antibacterial (both bactericidal and synergistic) potentials against drug-resistant pathogens have been discussed. The review also provides deeper mechanistic insights into the mechanisms of the action of PANs against a variety of drug-resistant pathogens with a critical evaluation of efflux pumps, cell membrane permeability, biofilm, and quorum sensing inhibition. We also discuss the use of PANs as drug carriers. This review also discusses possible cytotoxicities related to the therapeutic use of PANs and effective strategies to overcome this. Recent developments, success stories, challenges, and prospects are also presented.

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Using visible light to activate antiviral and antimicrobial properties of TiO2 nanoparticles in paints and coatings: focus on new developments for frequent-touch surfaces in hospitals

et al. 2023

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The COVID-19 pandemic refocused scientists the world over to produce technologies that will be able to prevent the spread of such diseases in the future. One area that deservedly receives much attention is the disinfection of health facilities like hospitals, public areas like bathrooms and train stations, and cleaning areas in the food industry. Microorganisms and viruses can attach to and survive on surfaces for a long time in most cases, increasing the risk for infection. One of the most attractive disinfection methods is paints and coatings containing nanoparticles that act as photocatalysts. Of these, titanium dioxide is appealing due to its low cost and photoreactivity. However, on its own, it can only be activated under high-energy UV light due to the high band gap and fast recombination of photogenerated species. The ideal material or coating should be activated under artificial light conditions to impact indoor areas, especially considering wall paints or frequent-touch areas like door handles and elevator buttons. By introducing dopants to TiO 2 NPs, the bandgap can be lowered to a state of visible-light photocatalysis occurring. Naturally, many researchers are exploring this property now. This review article highlights the most recent advancements and research on visible-light activation of TiO 2 -doped NPs in coatings and paints. The progress in fighting air pollution and personal protective equipment is also briefly discussed. Graphical Abstract Indoor visible-light photocatalytic activation of reactive oxygen species (ROS) over TiO 2 nanoparticles in paint to kill bacteria and coat frequently touched surfaces in the medical and food industries.

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Antimicrobial and biofilm-disrupting nanostructured TiO2 coating demonstrating photoactivity and dark activity

Cited by 9 publications

References 50 publications

Copper and nanostructured anatase rutile and carbon coatings induce adaptive antibiotic resistance

Copper and nanostructured anatase rutile and carbon coatings induce adaptive antibiotic resistance

Combating Drug-Resistant Bacteria Using Photothermally Active Nanomaterials: A Perspective Review

Using visible light to activate antiviral and antimicrobial properties of TiO2 nanoparticles in paints and coatings: focus on new developments for frequent-touch surfaces in hospitals

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