Antimicrobial and bacteriostatic activity of surfactants against B. subtilis for microbial cleaner formulation

Vehapi, Meyrem; Özçimen, Didem

doi:10.1007/s00203-021-02328-0

Cited by 15 publications

(6 citation statements)

References 33 publications

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“…will undoubtably increase with PBS use. No AMR genes have been detected in any Bacilli isolates post-PBS application [72,73,77,82], and no PBS-derived Bacillus infections have been detected in any of the hospitalized patients [61,63,64,67,68]. Taken together, these results support the idea that PBS operates by a competitive exclusion mechanism that allows for the displacement of resident bacteria and pathogens, and the concomitant replacement with non-pathogenic, non-resistant Bacillus spp.…”

Section: Resultssupporting

confidence: 62%

“…Since then, eco-friendly detergents with high concentrations of apathogenic, food-grade bacterial spores of the Bacillus genus (namely B. subtilis, B. pumilus and B. megaterium species) have been the predominant PBS system investigated. Bacillus spores are effectively able to survive a wide range of temperatures and pH environments [66,67]. Once diluted and applied, Bacillus spores are able to germinate and quickly colonize dry inanimate surfaces, ultimately dominating the composition of the resident microbiome and competitively excluding pathogens from surviving and colonizing these high-touch surfaces [68].…”

Section: Introductionmentioning

confidence: 99%

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Probiotic-Based Sanitation in the Built Environment—An Alternative to Chemical Disinfectants

Ramos

Frantz

2023

Applied Microbiology

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The use of conventional chemical disinfectants is a common practice in built environments and has drastically increased in response to the COVID-19 pandemic. While effective for instantaneous disinfection, the application of chemical disinfectants to indoor surfaces is associated with recontamination and is prone to select for antimicrobial-resistant pathogens. In contrast, probiotic-based sanitation (PBS) relies on the premise that probiotic bacteria, namely apathogenic Bacillus spp., when combined with eco-friendly detergents and applied to indoor surfaces can outcompete and exclude pathogens. Recent in situ studies assessing PBS in healthcare settings have demonstrated overwhelmingly positive results, including significant reductions in pathogen burden, antimicrobial-resistant genes and nosocomial infections, yet these studies are limited in duration and scope. Here, we review results of Bacillus-based PBS in practice, identify knowledge gaps and discuss the considerations for the widespread use of PBS in built environments. In a time when indoor cleaning and disinfection has come to the forefront, PBS may offer an attractive, effective and sustainable alternative to conventional chemical disinfectants.

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Section: Resultssupporting

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Probiotic-Based Sanitation in the Built Environment—An Alternative to Chemical Disinfectants

Ramos

Frantz

2023

Applied Microbiology

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“…Wetting agents such as sodium lauryl sulfate, stretch powder, polyethylene glycol, calcium lignosulfonate, sodium carboxymethyl cellulose, sodium dodecylbenzene sulfonate, sodium tripolyphosphate, lignin, and sodium sulfonate can also be added in the formulations to reduce the interfacial tension between liquid and solid to increase the adhesion of liquid to solid surface [ 88 , 89 ]. In the storage and transportation of microbiological pesticides, the influence of a wetting agent on the water activity of microbiological pesticides should be considered.…”

Section: Application Of Adjuvants In Microbial Pesticide Formulationsmentioning

confidence: 99%

Towards Sustainable Green Adjuvants for Microbial Pesticides: Recent Progress, Upcoming Challenges, and Future Perspectives

et al. 2023

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Microbial pesticides can be significantly improved by adjuvants. At present, microbial pesticide formulations are mainly wettable powders and suspension concentrations, which are usually produced with adjuvants such as surfactants, carriers, protective agents, and nutritional adjuvants. Surfactants can improve the tension between liquid pesticides and crop surfaces, resulting in stronger permeability and wettability of the formulations. Carriers are inert components of loaded or diluted pesticides, which can control the release of active components at appropriate times. Protective agents are able to help microorganisms to resist in adverse environments. Nutritional adjuvants are used to provide nutrients for microorganisms in microbial pesticides. Most of the adjuvants used in microbial pesticides still refer to those of chemical pesticides. However, some adjuvants may have harmful effects on non-target organisms and ecological environments. Herein, in order to promote research and improvement of microbial pesticides, the types of microbial pesticide formulations were briefly reviewed, and research progress of adjuvants and their applications in microbial pesticides were highlighted, the challenges and the future perspectives towards sustainable green adjuvants of microbial pesticides were also discussed in this review.

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“…11 Furthermore, spores should remain metabolically active under harsh environmental conditions, making the bacterial strain appropriate for the formulation of stable and viable products. 12 Apart from the genetic approach, successful spore production and commercialization are dependent on the development of an optimal fermentation medium. The composition of the medium and the concentration of certain components are crucial for the sporulation process.…”

Section: Introductionmentioning

confidence: 99%

“…Generally, the industrial exploitation of the spores requires high cell densities and elevated sporulation productivity 11 . Furthermore, spores should remain metabolically active under harsh environmental conditions, making the bacterial strain appropriate for the formulation of stable and viable products 12 . Apart from the genetic approach, successful spore production and commercialization are dependent on the development of an optimal fermentation medium.…”

Section: Introductionmentioning

confidence: 99%

Multiple linear regression models to simulate spore yields of Bacillus amyloliquefaciens BS13 through optimization of medium composition

Kamoun

Weekers²,

Ayed

et al. 2022

Biotech and App Biochem

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Bacillus amyloliquefaciens is a food spoilage spore-forming bacterium. Its spores are useful for multiple biotechnological applications. Nevertheless, few reports are available regarding the achievement of a high cell density and good sporulation effectiveness under fermentation conditions. Therefore, the current study was designed to optimize a low-cost fermentation medium allowing the highest sporulation yield by B. amyloliquefaciens strain BS13. Our data revealed that tryptone and starch were the best carbon and energy sources. In addition, two nitrogen sources namely, corn steep liquor (CSL) and yeast extract (YE), allowed a significant enhancement of spore production and they were both retained for further optimization. A combination of CaCl 2 , MgSO 4 , and MnSO 4 showed a positive impact on spores' production. The composition of the optimized medium was (in g/L); tryptone 3, starch 15, CSL 13.5, YE 1.5, CaCl 2 0.1, MgSO 4 ⋅7H 2 O 0.012, and MnSO 4 ⋅7H 2 O 0.0012. Such medium was further validated in a 400-L fermentor. The spore yield by B. amyloliquefaciens strain BS13 was enhanced from 3.0 × 10 10 spores/mL under flask culture conditions to 6.2 × 10 10 spores/mL when cultures were performed on large scale. Therefore, strain BS13 spore preparation could be proposed as a promising probiotic and a biocontrol agent useful for plants, animals, and humans.

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Antimicrobial and bacteriostatic activity of surfactants against B. subtilis for microbial cleaner formulation

Cited by 15 publications

References 33 publications

Probiotic-Based Sanitation in the Built Environment—An Alternative to Chemical Disinfectants

Probiotic-Based Sanitation in the Built Environment—An Alternative to Chemical Disinfectants

Towards Sustainable Green Adjuvants for Microbial Pesticides: Recent Progress, Upcoming Challenges, and Future Perspectives

Multiple linear regression models to simulate spore yields of Bacillus amyloliquefaciens BS13 through optimization of medium composition

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