Antimicrobial and antioxidant effect of methanolic Crinum jagus bulb extract in wound healing

Udegbunam, Sunday Ositadimma; Udegbunam, Rita Ijeoma; Nnaji, T. O.; Anyanwu, Madubuike Umunna; Kene, Raphel Okoli Chukwujekwu; Anika, Silavanus Maduka

doi:10.5455/jice.20150511022858

Cited by 19 publications

(17 citation statements)

References 47 publications

(76 reference statements)

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“…The storage conditions of plant material (whole/extract) have been shown to affect the result of antimicrobial studies by impacting microbial efficacy [ 16 , 64 , 73 ]. Some papers on Nigerian plants reported storage of extract in refrigerator at 4°C [ 18 , 59 , 60 , 74 ]. This storage condition is good because the activity/growth of possible extract-contaminating organisms would be inhibited and this in a way enhances the reliability of the result [ 16 , 75 ].…”

Section: Experimental Approach Used In Antimicrobial Investigation Ofmentioning

confidence: 99%

“…[ 57 , 102 , 104 , 178 - 180 ]. Some specific species have been investigated by several researchers such as Vernonia amygdalina [ 86 , 94 , 97 , 105 , 141 , 179 , 181 , 182 ], Psidium guajava [ 87 , 121 , 163 , 183 ], Jatropha curcas [ 88 , 138 , 184 ], Crinum jagus [ 59 , 118 , 127 ], Moringa oleifera [ 147 , 185 , 186 ], Anogeissus leiocarpus [ 50 , 111 , 171 , 138 , 153 , 167 , 186 - 189 ], Gongronema latifolium [ 128 , 136 ], and Fagara ( Zanthoxylum ) zanthoxyloides [ 39 , 190 ] while some papers were dedicated to the study of specific species such as Terminalia avicennioides [ 58 , 71 , 117 ], Stachytarpheta angustifolia [ 69 , 70 ], and Struchium sparganophora [ 191 , 192 ].…”

Section: Antimicrobial Investigation On Nigerian Plant Extractsmentioning

confidence: 99%

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Antimicrobial activity of Nigerian medicinal plants

Anyanwu¹,

Okoye²

2017

J Intercult Ethnopharmacol

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Antimicrobial resistance (AMR) is currently one of the major threats facing mankind. The emergence and rapid spread of multi- and pan-drug-resistant organisms (such as vancomycin-, methicillin-, extended-spectrum β-lactam-, carbapenem- and colistin-resistant organisms) has put the world in a dilemma. The health and economic burden associated with AMR on a global scale are dreadful. Available antimicrobials have been misused and are almost ineffective with some of these drugs associated with dangerous side effects in some individuals. Development of new, effective, and safe antimicrobials is one of the ways by which AMR burden can be reduced. The rate at which microorganisms develop AMR mechanisms outpaces the rate at which new antimicrobials are being developed. Medicinal plants are potential sources of new antimicrobial molecules. There is renewed interest in antimicrobial activities of phytochemicals. Nigeria boasts of a huge heritage of medicinal plants and there is avalanche of researches that have been undertaken to screen antimicrobial activities of these plants. Scientific compilation of these studies could provide useful information on the antimicrobial properties of the plants. This information can be useful in the development of new antimicrobial drugs. This paper reviews antimicrobial researches that have been undertaken on Nigerian medicinal plants.

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Section: Experimental Approach Used In Antimicrobial Investigation Ofmentioning

confidence: 99%

Section: Antimicrobial Investigation On Nigerian Plant Extractsmentioning

confidence: 99%

Antimicrobial activity of Nigerian medicinal plants

Anyanwu¹,

Okoye²

2017

J Intercult Ethnopharmacol

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“…Variation in MIC and MBC of the extract against the organisms may be due to differences in their resistance to the antibacterial phytoconstituents in the plant extract. The highest MIC and MBC for P. aeruginosa is not suprising, because, apart from being a septic wound isolate, most pseudomonal isolates are inherently resistant to most antibacterial agents including plant extracts that possessed antibacterial activity (Li et al, 1994;Udegbunam et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of wound healing and antibacterial properties of methanolic root extract of <i>Stephania dinklagei</i> (Engl.) Diels

Udegbunam¹,

Igbokwe²,

Udegbunam³

et al. 2015

Afr. J. Trad. Compl. Alt. Med.

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Background: Stephania dinklagei Diels (Engl.) is used in folkloric medicine in Southeastern Nigeria for the treatment of wounds and some bacterial-associated infections. This study evaluated the wound healing and antibacterial potential of Stephania dinklagei to validate or invalidate its folkloric use. Materials and Methods:The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of methanolic extract of S. dinklagei root (MESDR) against Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Klebsiella spp. was determined by macro broth dilution. The extract at 20% and 10% were dosed orally to rats at 300mg/kg body weight (bw) in incision and dead space wound healing model to determine wound tensile strength and granulation tissue weight, respectively. The same extract concentrations were applied topically in excision wound model to determine the rate of wound contraction and epithelialization. Activities of superoxide dismutase (SOD) and catalase (CAT), and the levels of total protein (TP), malondialdehyde (MAL), hydroxyproline (HYP) and hexosamine (HEX) in excision wound biopsies were determined at days 7 and 14 post-wounding (pw). In the excision wound model, the extract concentrations were compared with gentamicin sulphate. Results: The MIC of S. dinklagei extract against P. aeruginosa, S. aureus, B. subtilis, E. coli and Klebsiella spp. were 8mg/ml, 3 mg/ml, 5mg/ml, 6mg/ml and 7mg/ml, respectively, while the corresponding MBC were 10 mg/ml, 5 mg/ml, 7mg/ml, 8mg/ml, and 9 mg/ml, respectively. The 20% extract gave significantly (P<0.05) higher tensile strength and granulation tissue weight than the 10% and gentamicin sulphate. Wound contraction and epithelialization occurred significantly (P<0.05) better and faster in wounds of animals treated with the 20% extract and gentamicin sulphate compared to those treated with 10% extract. TP of animals treated with 20% extract and those treated with the reference drug did not vary significantly (P>0.05) at day 14 pw. SOD and CAT activities, and MDA and HEX level of all the groups did not vary significantly (P>0.05) at day 14 pw. HYP level of the extract-treated groups significantly (P<0.05) decreased against the control. No significant difference existed in HYP level between the extract-treated groups. Conclusions: S. dinklagei possess antibacterial and wound healing properties which are comparable to those of gentamicin sulphate.

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“…Inhibitory zone around the disc was measured and expressed in mm as an index of anti-microbial property. [36][37][38]…”

Section: Anti-microbial Activitymentioning

confidence: 99%

Exploration of Bio-Active Phytocomponents and Evaluation of Anti-Microbial Potential of Strychnos Potatorum

Murugan¹,

Venkatachalam²

2019

Int. Res. J. Pharm.

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Research interest towards herbal medicine grabs major attention in recent times due to its innumerable role in the process of drug discovery. It is evident that traditional medicines contribute as a therapeutic ailment for treating dreadful disease. Present investigation aimed at exploring the major phytocomponents present in the herb Strychnos potatorum (SP) and to screen its possible anti-microbial activity against selective pathogens. Ethanol (EESP), Aqueous (AESP) and Hydro alcoholic (HAESP) extracts were screened using biochemical, GCMS and FTIR analysis. Outcome of the study clearly signifies the presence of bioactive components such as alkaloids, flavonoids, tannins, phenols and saponin in the extracts. Results of GCMS justify the existence of ascorbic, oleic and erucic acid in EESP. Further higher level of active components was reports in EESP. FTIR study evidents the presence of major active functional groups in the extracts. Anti-microbial evaluation reveals that the EESP exhibits maximum zone of inhibition against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Enterococcus faecalis and Klebsiella pneumonia. Present study provides an evidencebased data's with respect to the category and nature of phytocomponents present in the herb Strychnos potatorum and hence the promising anti-microbial activity may due to its biologically active phyto therapeutics which may render beneficial activity against infectious and cardiovascular diseases subjected to proper preclinical justification in near future.

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Antimicrobial and antioxidant effect of methanolic Crinum jagus bulb extract in wound healing

Cited by 19 publications

References 47 publications

Antimicrobial activity of Nigerian medicinal plants

Antimicrobial activity of Nigerian medicinal plants

Evaluation of wound healing and antibacterial properties of methanolic root extract of <i>Stephania dinklagei</i> (Engl.) Diels

Exploration of Bio-Active Phytocomponents and Evaluation of Anti-Microbial Potential of Strychnos Potatorum

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