Antimicrobial and anticancer activities of Periplaneta americana tissue lysate: An in vitro study

Hamdy, Basma; Amer, Asmaa; Azzam, May; El‐Sattar, Nour E. A. Abd; Mahmoud, Dalia; Al-Ashaal, Sara A.; Al-Khalaf, Areej A.; Hozzein, Wael N.

doi:10.1016/j.jksus.2022.102095

Cited by 17 publications

(9 citation statements)

References 33 publications

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“…Sections were then stained three times with uranyl acetate and lead citrate. A JEOL-JEM 1010 transmission electron microscope was employed by the Regional Center for Mycology and Biotechnology (RCMB), Al- Azhar University, to examine stained slices at a voltage of 70 kV [ 39 , 40 , 41 ].…”

Section: Methodsmentioning

confidence: 99%

Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study

Hashem

Saied

Amin

et al. 2022

JFB

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Currently, nanoparticles and nanomaterials are widely used for biomedical applications. In the present study, silver nanoparticles (AgNPs) were successfully biosynthesized using a cell-free extract (CFE) of Bacillus thuringiensis MAE 6 through a green and ecofriendly method. The size of the biosynthesized AgNPs was 32.7 nm, and their crystalline nature was confirmed by XRD, according to characterization results. A surface plasmon resonance spectrum of AgNPs was obtained at 420 nm. Nanoparticles were further characterized using DLS and FTIR analyses, which provided information on their size, stability, and functional groups. AgNPs revealed less cytotoxicity against normal Vero cell line [IC50 = 155 μg/mL]. Moreover, the biosynthesized AgNPs exhibited promising antifungal activity against four most common Aspergillus, including Aspergillus niger, A. terreus, A. flavus, and A. fumigatus at concentrations of 500 μg/mL where inhibition zones were 16, 20, 26, and 19 mm, respectively. In addition, MICs of AgNPs against A. niger, A. terreus, A. flavus, and A. fumigatus were 125, 62.5, 15.62, and 62.5 μg/mL, respectively. Furthermore, the ultrastructural study confirmed the antifungal effect of AgNPs, where the cell wall’s integrity and homogeneity were lost; the cell membrane had separated from the cell wall and had intruded into the cytoplasm. In conclusion, the biosynthesized AgNPs using a CFE of B. thuringiensis can be used as a promising antifungal agent against Aspergillus species causing Aspergillosis.

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Section: Methodsmentioning

confidence: 99%

Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study

Hashem

Saied

Amin

et al. 2022

JFB

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“…The homogenates were centrifuged, and the supernatant was stored at room temperature for further analysis. The 3rd part was fixed in glutaraldehyde and used for TEM studies 56 .…”

Section: Methodsmentioning

confidence: 99%

Mycosynthesis of selenium nanoparticles using Penicillium tardochrysogenum as a therapeutic agent and their combination with infrared irradiation against Ehrlich carcinoma

EL-Sayed,

El-Sheekh,

Abo-Neima

2024

Sci Rep

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Over the past years, the assessment of myco-fabricated selenium nanoparticles (SeNPs) properties, is still in its infancy. Herein, we have highly stable myco-synthesized SeNPs using molecularly identified soil-isolated fungus; Penicillium tardochrysogenum OR059437; (PeSeNPs) were clarified via TEM, EDX, UV–Vis spectrophotometer, FTIR and zeta potential. The therapeutic efficacy profile will be determined, these crystalline PeSeNPs were examined for antioxidant, antimicrobial, MIC, and anticancer potentials, indicating that, PeSeNPs have antioxidant activity of (IC50, 109.11 μg/mL) using DPPH free radical scavenging assay. Also, PeSeNPs possess antimicrobial potential against Penicillium italicum RCMB 001,018 (1) IMI 193,019, Methicillin-Resistant Staphylococcus aureus (MRSA) ATCC 4330 and Porphyromonas gingivalis RCMB 022,001 (1) EMCC 1699; with I.Z. diameters and MIC; 16 ± 0.5 mm and MIC 500 µg/ml, 11.9 ± 0.6 mm, 500 µg/ml and 15.9±0.6 mm, 1000 µg/ml, respectively. Additionally, TEM micrographs were taken for P. italicum treated with PeSeNPs, demonstrating the destruction of hyphal membrane and internal organelles integrity, pores formation, and cell death. PeSeNP alone in vivo and combined with a near-infrared physiotherapy lamp with an energy intensity of 140 mW/cm2 showed a strong therapeutic effect against cancer cells. Thus, PeSeNPs represent anticancer agents and a suitable photothermal option for treating different kinds of cancer cells with lower toxicity and higher efficiency than normal cells. The combination therapy showed a very large and significant reduction in tumor volume, the tumor cells showed large necrosis, shrank, and disappeared. There was also improvement in liver ultrastructure, liver enzymes, and histology, as well as renal function, urea, and creatinine.

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“…Then, sections were double stained in lead citrate and uranyl acetate. A JEOL - JEM 1010 transmission electron microscope at 70 kV was used to observe the stained sections at The Regional Center for Mycology and Biotechnology (RCMB), Al- Azhar University [ 33 –[ 34 ]– 35 ].…”

Section: Methodsmentioning

confidence: 99%

Synergistic antibacterial effects of Ulva lactuca methanolic extract alone and in combination with different antibiotics on multidrug-resistant Klebsiella pneumoniae isolate

2023

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Various antibiotics are available, including gentamicin, chloramphenicol, ampicillin, amoxicillin, and streptomycin, but they have some restrictions. Many microorganisms are resistant to these medications. A new antimicrobial source must be found or developed to solve this issue. Inhere, extract from seaweeds Ulva lactuca was investigated for its antibacterial activity using a well diffusion assay against Klebsiella pneumoniae, and a promising inhibition zone diameter was recorded to be 14.04 mm. The biochemical structure of the antibacterial compound was determined via GC-MS and FTIR analysis. Also, a micro-dilution assay was used to calculate the minimum concentration that makes inhibition (MIC) to be 1.25 mg/ml from U. extract reliable to prevent the visibility of any bacterial growth, this was followed by examining the antibacterial effect of U. Lactuca methanolic extract alone and the synergetic effect of U. Lactuca methanolic extract in combination with two different antibiotics (gentamicin and chloramphenicol). This was assayed by the agar well diffusion method to achieve promising and strong inhibiting power against K. pneumoniae. It was deduced that the maximum synergism could be achieved by adding 2.5 mg/ml of Ulva methanolic extract to gentamicin (4 µg/ml), and the results were illustrated obviously via transmission electron microscope in which severe morphological deteriorations were experienced by the treated cells. From this study, we can conclude that U. lactucae extract has the power to aid antibiotics in reducing the growth of pathogenic K. pneumoniae.

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Antimicrobial and anticancer activities of Periplaneta americana tissue lysate: An in vitro study

Cited by 17 publications

References 33 publications

Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study

Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study

Mycosynthesis of selenium nanoparticles using Penicillium tardochrysogenum as a therapeutic agent and their combination with infrared irradiation against Ehrlich carcinoma

Synergistic antibacterial effects of Ulva lactuca methanolic extract alone and in combination with different antibiotics on multidrug-resistant Klebsiella pneumoniae isolate

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