Antimicrobial and antibiofilm activities and bovine serum albumin binding properties of benzimidazolium derivative NHC salts and their Ag(I)‐NHC complexes

Abstract

Benzimidazole type N‐heterocyclic carbene molecules and their metal complexes have well‐known biological activity. Elemental silver and silver complexes also have antibacterial and antifungal activity. In this study, silver complexes with 1‐allyl 5,6‐dimethylbenzimidazolium derivative ligands were analyzed for their antimicrobial and antibiofilm activities. Additionally, bovine serum albumin (BSA) binding properties of the molecules were analyzed by spectrophotometric methods, and the details of interactions of the molecules with BSA were investigated by molecular docking methods. While N‐heterocyclic carbene salts showed moderate antifungal and antibiofilm activity, silver complexes of these salts have strong antimicrobial activity with <1.9–15.6 μg/ml minimal inhibitory concentration (MIC) values. On the other hand, carbene salts have better affinity against BSA than the complexes. Additionally, the interaction of the optimized complexes with SarA and BSA were analyzed by molecular docking methods. According to in vitro and in silico evaluation, the studied Ag complexes may be utilized for advanced studies.

Advancing Pyrrole Synthesis through DDQ Catalysis: A Comprehensive Research Incorporating DFT, ADMT, and Molecular Docking Analysis

“…Therefore, the interaction properties of serum albumin have frequently been studied. As is generally recognized, BSA is an adequate model protein for study and comparison given its structural similarity to human serum albumin [69] . Gammal et al analyzed the interactions between mebendazole and BSA and recorded −6.9 kcal/mol binding affinity with the site I of subdomain IIA of BSA which is included in Arg194, Arg198, Ala290, Trp213, Arg217, Leu259, Leu237, Lys221, Ile289, Ile263, and Asp450 [70] .…”

“…As is generally recognized, BSA is an adequate model protein for study and comparison given its structural similarity to human serum albumin. [69] Gammal et al analyzed the interactions between mebendazole and BSA and recorded À 6.9 kcal/mol binding affinity with the site I of subdomain IIA of BSA which is included in Arg194, Arg198, Ala290, Trp213, Arg217, Leu259, Leu237, Lys221, Ile289, Ile263, and Asp450. [70] Wani et al recorded the interactions between indole derivative molecules and Ala212, Ala209, Leu454, Leu346, Leu480, Leu197, Val342, Val481, and Phe205 amino acids of BSA with À 28.28kJ/mol binding affinity.…”

Comprehensive Insights into Medicinal Research on Imidazole-Based Supramolecular Complexes

“…A p -isobutylbenzyl-substituted benzimidazole silver (I) compound II156 displayed excellent biological activity, with a MIC value below 1.9 μg/mL. Compound II156 could bind well with BSA by forming hydrogen bonds [ 314 ].…”

Green Synthesis of Pyrrole Derivatives Catalyzed by Molecular Sieves: DFT, ADMT, and Molecular Docking Investigations

“…[25] Bovine Serum Albumin (BSA) which is very similar to Human Serum Albumin is a model protein that is frequently analyzed for different research. [26] Moreover, since this protein is an important indicator of cow health, milk, and meat quality, albumin analysis has been frequently performed in recent years. [27] Therefore, the interactions of bovine serum albumin are important in various industries such as pharmaceutical, clinical, and food.…”

“…The serum albumin concentration in human blood plasma and other biological fluids is often tested as many compounds are transported with this protein in the blood [25] . Bovine Serum Albumin (BSA) which is very similar to Human Serum Albumin is a model protein that is frequently analyzed for different research [26] . Moreover, since this protein is an important indicator of cow health, milk, and meat quality, albumin analysis has been frequently performed in recent years [27] .…”