Antimicrobial and anti-inflammatory drug-delivery systems at endodontic reparative material: Synthesis and characterization

Cuppini, Marla; Zatta, Kelly Cristine; Mestieri, Letícia Boldrin; Grecca, Fabiana Soares; Leitune, Vicente Castelo Branco; Guterres, Sílvia Stanisçuaski; Collares, Fabrício Mezzomo

doi:10.1016/j.dental.2019.01.002

Cited by 20 publications

(13 citation statements)

References 57 publications

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“…In regenerative medicine, cell migration and adhesion of stem cells are key phenomena during apical healing, because of their participation on local tissue repairs in a variety of physiological and pathological conditions [30]. In this study, it was verified that Bio-C Sealer and TotalFill BC Sealer supported cell migration; this finding could be related to the positive effects of calcium ions on cell migration [28].…”

Section: Discussionmentioning

confidence: 60%

Comparative Cytocompatibility and Mineralization Potential of Bio-C Sealer and TotalFill BC Sealer

et al. 2019

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The aim of this study was to investigate the cytocompatibility and mineralization potential of two premixed hydraulic endodontic sealers compared with an epoxy resin-based root canal sealer. The cellular responses and mineralization capacity were studied in human periodontal ligament stem cells (hPDLSCs) that were exposed to premixed hydraulic sealers, Bio-C Sealer (Angelus, Londrína, PR, Brazil), TotalFill BC Sealer (FKG Dentaire SA, La-Chaux-de-fonds, Switzerland) and an epoxy resin-based material, AH Plus (Dentsply De Trey, Konstanz, Germany). Non-exposed cultures served as the control. The endodontic sealers were assessed using scanning electron microscopy (SEM) and energy dispersive X-ray microanalysis (EDX). Statistical analyses were done using Analisis of Variance (ANOVA), with Bonferroni adjusted pairwise comparison (p = 0.05). AH Plus reduced cell viability and cell migration, whereas increased cell viability and cell migration were observed in the Bio-C Sealer and the TotalFill BC Sealer (p < 0.05). The lowest cell attachment and spreading were observed for all concentrations of AH Plus, whereas the highest were observed for TotalFill BC Sealer. At the end of 21 days, only the Bio-C Sealer and the TotalFill BC Sealer supported matrix mineralization (p < 0.05). Additionally, SEM-EDX revealed high content of calcium, oxygen, and silicon in the Bio-C Sealer and the TotalFill BC Sealer. Based on the results from this study, Bio-C Sealer and TotalFill BC Sealer demonstrated better cytocompatibility in terms of cell viability, migration, cell morphology, cell attachment, and mineralization capacity than AH Plus.

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Section: Discussionmentioning

confidence: 60%

Comparative Cytocompatibility and Mineralization Potential of Bio-C Sealer and TotalFill BC Sealer

et al. 2019

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“…Genipin-crosslinked CL-PVMMA sponges prepared as in this study have enough cytocompatibility, mechanical strength, plasticity, and ability to absorb fluids without disintegrating to support sustained drug delivery and cell growth for a sufficient period of time [ 11 ]. In addition to their antimicrobial efficacy, sponges are also being investigated in regenerative endodontics due to their cytocompatibility and ability to stimulate nucleation sites [ 25 ]. Nowadays, several mechanisms for sustained drug release are being investigated.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Development of a New Sponge-Based Delivery System for Intracanal Antimicrobial Administration in Endodontic Treatment

Ruíz‐Piñón

Gancedo-Gancedo

Prado

et al. 2021

JCM

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This study aimed to evaluate the in vitro performance of collagen-based sponges as a drug delivery system for intracanal antimicrobial administration. Four groups of loaded collagen-based sponges (A, 0.3% w/v amoxicillin trihydrate: potassium clavulanate (4:1); B, 0.03% w/v chlorhexidine gluconate [CHX]; C, 0.3% w/v amoxicillin trihydrate: potassium clavulanate (4:1) and 0.03% w/v CHX; D, 1% w/v amoxicillin trihydrate: potassium clavulanate (4:1) and 0.03% w/v CHX) were designed. Release kinetics were tested in vitro on cultures in Petri dishes, and the effect on bacterial biofilms was studied ex vivo on 114 extracted human single-rooted teeth. Biofilm formation was tested by scanning electron microscopy (SEM). Collagen sponges containing amoxicillin and chlorhexidine showed a time-sustained antimicrobial effect in vitro and were also able to destroy mature biofilms ex vivo. This datum was validated by means of SEM-based study of E. faecalis and S. aureus biofilms.

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“…With recent advances in nanoscale manufacturing, an increased number of approaches have utilized the opportunities given by nano-structured materials to effectively deliver drugs into the dental pulp. Cuppini et al (2019) developed a tricalcium-phosphate based paste containing amoxicillin microspheres, calcium tungstate and indomethacin nanocapsules to deliver antimicrobial and anti-inflammatory compounds to the defect site. Zhu et al (2017) designed calcium silicate nanoparticles in combination with silver and zinc as a nano-disinfectant for dentin tubules and root canals.…”

Section: Novel Approaches For Dental Pain Treatment and Beyond: Regenmentioning

confidence: 99%

Potential Novel Strategies for the Treatment of Dental Pulp-Derived Pain: Pharmacological Approaches and Beyond

2019

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The diagnosis and management of pain is an everyday occurrence in dentistry, and its effective control is essential to ensure the wellbeing of patients. Most tooth-associated pain originates from the dental pulp, a highly vascularized and innervated tissue, which is encased within mineralized dentin. It plays a crucial role in the sensing of stimuli from the local environment, such as infections (i.e. dental caries) and traumatic injury, leading to a local inflammatory response and subsequently to an increase in intra-pulp pressure, activating nerve endings. However, thermal, chemical, and mechanical stimuli also have the ability to generate dental pulp pain, which presents mechanisms highly specific to this tissue and which have to be considered in pain management. Traditionally, the management of dental pulp pain has mostly been pharmacological, using non-steroidal anti-inflammatory drugs (NSAIDs) and opioids, or restorative (i.e. removal of dental caries), or a combination of both. Both research areas continuously present novel and creative approaches. This includes the modulation of thermo-sensitive transient receptor potential cation channels (TRP) by newly designed drugs in pharmacological research, as well as the use of novel biomaterials, stem cells, exosomes and physical stimulation to obtain pulp regeneration in regenerative medicine. Therefore, the aim of this review is to present an up-to-date account of causes underlying dental pain, novel treatments involving the control of pain and inflammation and the induction of pulp regeneration, as well as insights in pain in dentistry from the physiological, pharmacological, regenerative and clinical perspectives.

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Antimicrobial and anti-inflammatory drug-delivery systems at endodontic reparative material: Synthesis and characterization

Cited by 20 publications

References 57 publications

Comparative Cytocompatibility and Mineralization Potential of Bio-C Sealer and TotalFill BC Sealer

Comparative Cytocompatibility and Mineralization Potential of Bio-C Sealer and TotalFill BC Sealer

In Vitro Development of a New Sponge-Based Delivery System for Intracanal Antimicrobial Administration in Endodontic Treatment

Potential Novel Strategies for the Treatment of Dental Pulp-Derived Pain: Pharmacological Approaches and Beyond

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