Antimicrobial and anti-adhesive potential of a biosurfactant Rufisan produced by Candida lipolytica UCP 0988

Abstract

In the last years, researches developed with biosurfactants for application in the medical area have been revealing the promising biological activities of these biomolecules. In this work the antimicrobial and anti-adhesive properties of a biosurfactant Rufisan isolated from the yeast Candida lipolytica UCP 0988, growth in a medium supplemented with ground nut refinery residue was determined against several microorganisms. The biosurfactant was able to reduce the water surface tension from 70 to 25.3 mN/m and showed a critical micelle concentration (CMC) of 0.03%. The biosurfactant was isolated after 72 h of fermentation and was tested in concentrations varying from 0.75 to 12 mg/l. The highest antimicrobial activities were observed against Streptococcus agalactiae, Streptococcus mutans, Streptococcus mutans NS, Streptococcus mutans HG, Streptococcus sanguis 12, Streptococcus oralis J22 at a concentration superior to the biosurfactant critical micelle concentration. Moreover, the biosurfactant showed anti-adhesive activity against most of the microorganisms tested. As far as we know, this is the first compilation of data on antimicrobial and anti-adhesive activities of a biosurfactant obtained from a Candida strain against such a broad group of microorganisms. The results obtained in this work showed that the biosurfactant from C. lipolytica is a potential antimicrobial and/or anti-adhesive agent for several biomedical applications.

Yarrowia lipolytica: Safety assessment of an oleaginous yeast with a great industrial potential

“…Yarrowia lipolytica seems to produce a variety of different emulsifiers, for example Liposan isolated from cultures grown on hexadecane, Yansan from cultures grown on glucose or Rufisan from cultures grown on soybean oil refinery residue (Amaral et al, 2006;Cirigliano & Carman, 1985;Fontes et al, 2010;Rufino et al, 2011).…”

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“…Пізніше з'явились повідомлення про синтез на олієвмісних відхо дах рамноліпідів Thermus thermophilus HB8 [13] і руфісану C. lipolytica UCP0988 [18]. Руфісан, у концентрації 6-12 мг/л, проявляв ефективну антимікробну дію щодо представників роду Streptococcus і Lactobacil-lus, проте практично не інгібував ріст C. albicans, E. ^li, Staphylococ cus аureus, Pseudomonas aeruginosa та Staphylococcus epidermidis (18 %) [18]; рамноліпід Thermus thermophilus HB8 пригнічував ріст Micrococcus lysodeikticus [13].…”

“…Зазначимо, що у літературі відомості про антиадгезивні властивості ПАР, синтезованих на олієвмісних промислових відходах та пересмаженій олії, є вкрай обмеженими. У праці [18] цтва соєвої олії. Руфісан знижував адгезію бактерій роду Streptococcus і Lactobacillus на полістиролових пластинках.…”

Production of bioemulsifiers by Yarrowia lipolytica in sea water using diesel oil as the carbon source

“…The authors determined a surface tension reduction of 59% when palm oil was added at 2 kg/m 3 .day with a cycle time of 2 days/cycle. Rufino et al (2011) Sarubbo et al (2007), Candida lipolytica synthesized a surfactant in a cultivation medium supplemented with canola oil and glucose as carbon sources after 48 h of fermentation. The cellfree broth was particularly influenced by the addition of salt, the pH and temperature depending on the emulsified substrate (hexadecane or a vegetable oil).…”