Antimicrobial activity of β-lapachone encapsulated into liposomes against meticillin-resistant Staphylococcus aureus and Cryptococcus neoformans clinical strains

Cavalcanti, Isabella Macário Ferro; Neto, João Gomes Pontes; Kocerginsky, P.O.; Bezerra-Neto, A.M.; Lima, J.L.C.; Nogueira, Mariane Cajubá de Britto Lira; Maciel, Maria Amélia Vieira; Neves, Rejane Pereira; Pimentel, Maria Fernanda; Santos-Magalhães, Nereide Stela

doi:10.1016/j.jgar.2015.03.007

Cited by 26 publications

(11 citation statements)

References 29 publications

(34 reference statements)

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“…The in vitro antimicrobial activity of VAN, UA and UA-lipo was evaluated by the microdilution method according to CLSI (2014). The antimicrobial activity of β-lap and β-lap-lipo against these MRSA clinical isolates has been previously reported by our research group (Cavalcanti et al, 2015). However their interactions with vancomycin have not yet been investigated.…”

Section: Determination Of Minimum Inhibitory Concentration (Mic) and mentioning

confidence: 99%

Interaction study between vancomycin and liposomes containing natural compounds against methicillin-resistant Staphylococcus aureus clinical isolates

Cavalcanti

Menezes

Campos

et al. 2018

Braz. J. Pharm. Sci.

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The treatment of infections caused by resistant microorganisms is limited, and vancomycin (VAN) treatment failures for methicillin-resistant Staphylococcus aureus (MRSA) bacteremia are not uncommon, even when MRSA clinical isolates are susceptible to VAN. Thus, this study proposed the association of VAN with usnic acid and β-lapachone encapsulated into liposomes as a novel therapeutic option for infections caused by MRSA. Liposomes containing β-lap (β-lap-lipo) or usnic acid (UA-lipo) were prepared by the thin lipid film hydration method followed by sonication. Antimicrobial activity against MRSA clinical isolates was investigated by the microdilution method according to the Clinical and Laboratory Standards Institute (CLSI). The interaction studies were carried out using the checkerboard method and epsilometer test (Etest). The interaction between VAN and β-lap or β-lap-lipo was synergistic (FICI = 0.453 and FICI = 0.358, respectively). An additive interaction between VAN and UA (FICI = 0.515) was found. UA-lipo resulted in synergism with VAN (FICI = 0.276). The Etest reproduced the results obtained by the checkerboard method for approximately 82% of the analysis. Thus, the present study demonstrated that VAN in combination with UA-lipo, β-lap or β-lap-lipo synergistically enhanced antibacterial activity against MRSA.

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Section: Determination Of Minimum Inhibitory Concentration (Mic) and mentioning

confidence: 99%

Interaction study between vancomycin and liposomes containing natural compounds against methicillin-resistant Staphylococcus aureus clinical isolates

Cavalcanti

Menezes

Campos

et al. 2018

Braz. J. Pharm. Sci.

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show abstract

“…Due to adaptation, and exposure to various antibiotics, some strains become multiresistant to the therapies currently employed, especially carbapenems, and also adapt to the biofilm condition [14]. Thus, it is necessary to develop new therapeutic approaches able to exert not only antibacterial activity, but also antibiofilm since the latter is considered a challenge for eradication [15,16]. Therefore, the use of materials on a nanometric scale is a viable strategy for carrying biomolecules and drugs, as it provides advantages such as increased half-life time and systemic circulation time, greater contact between the compound and the pathogen, better bioavailability, and greater absorption, resulting in a better adherence to therapy and more efficient treatment [17,18].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and antibiofilm potential of silver nanoparticles against antibiotic-sensitive and multidrug-resistant Pseudomonas aeruginosa strains

et al. 2020

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Due to the severity of infections caused by P. aeruginosa and the limitations in treatment, it is necessary to find new therapeutic alternatives. Thus, the use of silver nanoparticles (AgNPs) is a viable alternative because of their potential actions in the combat of microorganisms, showing efficacy against Gram-positive and Gram-negative bacteria, including multidrug-resistant microorganisms (MDR). In this sense, the aim of this work was to conduct a literature review related to the antibacterial and antibiofilm activity of AgNPs against antibiotic-sensitive and multidrug-resistant Pseudomonas aeruginosa strains. The AgNPs are promising for future applications, which may match the clinical need for effective antibiotic therapy. The size of AgNPs is a crucial element to determine the therapeutic activity of nanoparticles, since smaller particles present a larger surface area of contact with the microorganism, affecting their vital functioning. AgNPs adhere to the cytoplasmic membrane and cell wall of microorganisms, causing disruption, penetrating the cell, interacting with cellular structures and biomolecules, and inducing the generation of reactive oxygen species and free radicals. Studies describe the antimicrobial activity of AgNPs at minimum inhibitory concentration (MIC) between 1 and 200 μg/mL against susceptible and MDR P. aeruginosa strains. These studies have also shown antibiofilm activity through disruption of biofilm structure, and oxidative stress, inhibiting biofilm growth at concentrations between 1 and 600 μg/mL of AgNPs. This study evidences the advance of AgNPs as an antibacterial and antibiofilm agent against Pseudomonas aeruginosa strains, demonstrating to be an extremely promising approach to the development of new antimicrobial systems.

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“…have investigated for their biological properties. Cavalcanti et al (2015) reported the antimicrobial activity of β-lapachone encapsulated into liposomes against methicillin-resistant Staphylococcus aureus and clinical strains of Cryptococcus neoformans.…”

Section: Introductionmentioning

confidence: 99%

Chemical composition, oviposition deterrent and larvicidal activities of the wood extracts of Tabebuia avellanedae from the Cerrado of Brazil

Borges¹,

Silva²,

Barros³

et al. 2018

J. Med. Plants Res.

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Tabebuia avellanedae is an important timber source belonging to the family of Bignoniaceae. The latter is known for its richness in terms of variety of bioactive chemical constituents, and it has been used in folk medicine for treatment of various diseases. The aim of this work was to investigate the chemical composition, oviposition deterrent and larvicidal activities of the wood extracts of T. avellanedae from the Cerrado of Brazil. Extracts of acetone, ethyl acetate and ethanol from T. avellanedae were obtained using various extraction methods. Quantitative analysis of phytochemical screening confirmed the presence of phenols and tannins in the wood extracts, however, anthraquinones, coumarins and alkaloids were absent. The toxicity of T. avellanedae extracts against 3rd instar larvae of Aedes aegypti using maceration and Soxhlet extraction methods was analyzed. The acetone and ethyl acetate extracts obtained by Soxhlet extraction were more toxic against 3rd instar A. aegypti larvae, with CL 50 of 100.1 and 151.0 μg/mL, respectively. The mortality values (LT 50 and LT 95) were 38.66 and 66.74 min for ethyl acetate extract, respectively, and 53.47 and 119.96 min for acetone extract, respectively. In all cases, the assay showed that all extracts presented mortality of 100% to 3rd instar larvae after 12 h. The oviposition assay showed that gravid A. aegypti females laid their eggs preferentially in the control ovitraps. The ethanol extract at 333.3 μg/mL strongly deterred oviposition by 89.89% while the ethyl acetate and acetone extracts presented 89.04 and 68.10% deterrence, respectively. The bioactive compounds in T. avellanedae make it a potential source for the control of A. aegypti vectors, without promoting deforestation of trees.

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Antimicrobial activity of β-lapachone encapsulated into liposomes against meticillin-resistant Staphylococcus aureus and Cryptococcus neoformans clinical strains

Cited by 26 publications

References 29 publications

Interaction study between vancomycin and liposomes containing natural compounds against methicillin-resistant Staphylococcus aureus clinical isolates

Interaction study between vancomycin and liposomes containing natural compounds against methicillin-resistant Staphylococcus aureus clinical isolates

Antibacterial and antibiofilm potential of silver nanoparticles against antibiotic-sensitive and multidrug-resistant Pseudomonas aeruginosa strains

Chemical composition, oviposition deterrent and larvicidal activities of the wood extracts of Tabebuia avellanedae from the Cerrado of Brazil

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