Antimicrobial activity of various extracts of Plectranthus ambionicus and Phyllanthus amarus

Abstract

Hexane, chloroform, ethyl acetate and methanol extracts of leaves of Plectranthus ambionicus and Phyllanthus amarus were screened for their antimicrobial activity against human pathogenic bacterial and fungal strains. Antimicrobial activity was carried out by disc diffusion method, determination of minimum inhibitory concentrations (MIC), minimum bactericidal concentrations (MBC) and minimum fungicidal concentrations (MFC) against four strains of Gram positive bacteria, three strains of Gram negative bacteria and three species of fungi. The antimicrobial activity of various extracts of Plectranthus ambionicus and Phyllanthus amarus showed varied levels of antimicrobial activity against the studied bacterial and fungal pathogens. The mean zone of inhibition produced by all the tested extracts ranged from 7.0 ± 0.50 mm to 29.5 ± 0.50 mm. The MIC, MBC and MFC values were between 15.62 and 1000 μg/mL. The ethyl acetate extract of Plectranthus ambionicus showed good antimicrobial activity with the highest mean zone of inhibition (29.5±0.5 mm), lowest MIC (15.62 μg/mL) and MBC (31.25 μg/mL) values followed by ethyl acetate extract of Phyllanthus amarus (23.6±0.50 mm; MIC=62.5; MBC=125 μg/mL) against Aspergillus fumigates.

Chemical Components and Biological Activities of the Genus Phyllanthus: A Review of the Recent Literature

“…Pathmavathi and Thamizhiniyan prepared various leaf extracts (methanolic, hexane, chloroform, and ethyl acetate) of P. amarus and Plectranthus ambionicus to study their antifungal and anti-microbial potential [ 110 ]. Four Gram positive and three Gram negative bacterial strains along with three species of fungi were tested to screen the best isolate among the various organic plant extracts.…”

Medicinal values of the genus Phyllanthus - A review

“…Further the authors proposed that in-depth studies and clinical trials using these oils could define their effectiveness to combat various skin conditions such as third degree burns, cuts, injuries and post-operative wounds infections caused by K. granulomatis as well as many related strains (Adesegun et al, 2016). Pathmavathi and Thamizhiniyan (2016) prepared various leaf extracts (methanolic, hexane, chloroform, and ethyl acetate) of P. amarus and Plectranthus ambionicus to study their antifungal and antimicrobial potential. Four Gram positive and three Gram negative bacterial strains along with three species of fungi were tested to screen the best isolate among the various organic plant extracts.…”