Antimicrobial activity of silver nanoparticles synthesized from <i>Ficus benghalensis</i> against human pathogens

Sudhakar, Tata; Balashanmugam, Pannerselvam; Premkumar, J.; Anisha, A; Karthika, D; Sapkota, Roshan; Rijal, Sakar

doi:10.5958/0974-360x.2017.00287.6

Cited by 7 publications

(5 citation statements)

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Section: Edx Analysismentioning

confidence: 87%

“…The signal showed that the mycosynthesized silver nanoparticles were free from impurities. Previous studies also reported that silver nanoparticles showed absorption peaks at 3 keV [50]. In another study, it was reported that when AgNPs were analyzed using EDX, the strong signal of Ag atoms showed the crystalline property and the presence of oxygen peaks along with the silver signals showed that AgNPs were capped by phytoconstituents through oxygen atoms [47,51].…”

Section: Edx Analysismentioning

confidence: 94%

“…The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) assay was performed to monitor the inhibitory activity of synthesized AgNPs. The different concentrations (10,30,50,75, and 100 µg/mL) of AgNPs were tested on the Vero cell line under in vitro conditions (Figure 12). Vero cells treated with mycosynthesized AgNPs and untreated Vero cells (control) incubated for 48 h were examined under an inverted microscope.…”

Section: In Vitro Cytotoxicitymentioning

confidence: 99%

“…A 96-well microtiter plate was loaded with cells (1 × 10 6 cells/mL) and incubated for 24 h at 37 • C in an incubator. After 24 h, the medium was removed, and the Vero cell lines were treated with different concentrations (10,30,50,75, and 100 µg/mL) of synthesized AgNPs in duplicate. Cells without treatment with synthesized AgNPs were recorded as the control, and the microplate was incubated for 24 h with 5% CO 2 .…”

Section: In Vitro Cytotoxicity Of Synthesized Agnpsmentioning

confidence: 99%

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Macrofungal Mediated Biosynthesis of Silver Nanoparticles and Evaluation of Its Antibacterial and Wound-Healing Efficacy

Vijayakumar,

Kim,

et al. 2024

IJMS

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Recently, the utilization of biological agents in the green synthesis of nanoparticles has been given interest. In this study, silver nanoparticles were synthesized from an aqueous extract of macrofungus (mushroom), namely Phellinus adamantinus, in a dark room using 20 µL of silver nitrate. Biosynthesized silver nanoparticles were confirmed by analyzing them using a UV-Vis (ultraviolet-visible) spectrophotometer. The synthesized silver nanoparticles were optimized at different pH and temperatures with various dosages of AgNO3 (silver nitrate) and fungal extracts. The synthesized AgNPs (silver nanoparticles) were characterized using TEM (transmission electron microscopy) and EDX (energy-dispersive X-ray) analyses, which confirmed the presence of silver nanoparticles. The size of the nanosilver particles was found to be 50 nm with higher stability. The mycosynthesized AgNPs showed effective antibacterial activity against strains of Gram-positive (Staphylococcus aureus and Bacillus subtilis) and Gram-negative (E. coli and Pseudomonas aeruginosa) bacteria. The minimum inhibitory concentration (MIC) was found to be 3.125 μg/mL by MIC assay. The MTT assay (3-[4,5-dimethylthiazol-2-yl] 2,5-diphenyl-2H-tetrazolium bromide) was performed to study cytotoxicity, and reduced cell viability was recorded at 100 μg/mL. Silver-Polygalacturonic acid-Polyvinyl alcohol ((Ag-PGA)-PVA) nanofiber was prepared using the electrospinning method. The in vitro wound scratch assay was demonstrated to study the wound-healing efficacy of the prepared nanofiber. The wound-healing efficacy of the AgNP-incorporated nanofiber was found to be 20% after 24 h. This study will lay a platform to establish a unique route to the development of a novel nanobiomaterial and its application in antibacterial and wound-healing therapy.

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Section: Edx Analysismentioning

confidence: 87%

Section: Edx Analysismentioning

confidence: 94%

Section: In Vitro Cytotoxicitymentioning

confidence: 99%

Section: In Vitro Cytotoxicity Of Synthesized Agnpsmentioning

confidence: 99%

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Macrofungal Mediated Biosynthesis of Silver Nanoparticles and Evaluation of Its Antibacterial and Wound-Healing Efficacy

Vijayakumar,

Kim,

et al. 2024

IJMS

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“…Green tea extract is an antioxidant and an excellent reducing agent. Preparing silver nanoparticles with green tea extract is a cost-effective, green, and environmentally friendly one-step synthesis [7]. Vitamin D-3 promotes wound healing and can easily be included into the oil phase of an emulgel due to its lipophilic nature [8].…”

Section: Introductionmentioning

confidence: 99%

Design, Development and Improvement of an Emulgel Containing Silver Nanoparticles and Vitamin D-3 for Its Potential to Accelerate the Healing of Wound

YADAV,

PANDEY,

KUKKAR

2024

Int J App Pharm

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Objective: The aim of this research work was to prepare a topical emulgel based dosage form incorporated with vitamin D-3 and silver nanoparticles to reduce the wound healing time in any kind of wound. Methods: Central Composite Design (CCD) was applied for the optimization of emulgel by using Design expert software. Three responses (pH, viscosity, and in vitro drug release) and two factors (Carbopol concentration and stirring duration) were chosen, and Statistical Analysis of Variance (ANOVA) revealed that all the factors were significantly affecting the responses. Silver Nanoparticles (SNPs) was prepared with Green Tea Extract (GTE) and evaluated for particle size, Poly Dispersity Index (PDI), zeta potential and Fourier Transform Infra-red (FTIR) spectroscopy and revealed that SNPs of desired range and stability have been synthesized. Here excision wound model was used to evaluate the wound healing activity of formulation in vivo. Results: Maximum in vitro release 88.2±2.1 has shown by the optimized formulation F13, pH and viscosity were also found in optimum range i.e., 6.2±0.4 and 1672±33 respectively, followed by Korsmeyer and Peppas model. Total eight groups were designed for animal study and silver sulphadiazine was used as marketed formulation. F13 formulation was further evaluated for in vivo data, it was revealed that emulgel loaded with high dose of vitamin D-3 along with silver nanoparticles has shown 100.5±1.7% wound contraction, while marketed formulation has shown 103.7±1.1% wound contraction, which was much similar with test formulation. Cytotoxic cell study was done using assay on chicken egg, formulation has not shown any cytotoxic behaviour like haemolysis and cell damage on chick embryo’s blood vessels. Accelerated stability study of the optimized formulation was also performed to check whether the formulation was stable or not and it was revealed that optimized formulation was found stable for the period of six months. Conclusion: It was revealed that emulgel loaded with high dose of vitamin D-3 and SNPs found suitable to accelerate wound healing and showed almost similar response in wound contraction on comparison with marketed formulation. This emulgel promised to controlled the delivery of the drug for the longer duration.

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Biogenic Synthesis, Characterization, Along with Investigation of Cytotoxicity and Antibacterial Activities of Silver Nanoparticles Using Twigs of Polyalthia sclerophylla

Mudhafar,

Hadi,

MahdiAlrubayi

et al. 2024

BioNanoSci.

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Antimicrobial activity of silver nanoparticles synthesized from Ficus benghalensis against human pathogens

Cited by 7 publications

References 0 publications

Macrofungal Mediated Biosynthesis of Silver Nanoparticles and Evaluation of Its Antibacterial and Wound-Healing Efficacy

Macrofungal Mediated Biosynthesis of Silver Nanoparticles and Evaluation of Its Antibacterial and Wound-Healing Efficacy

Design, Development and Improvement of an Emulgel Containing Silver Nanoparticles and Vitamin D-3 for Its Potential to Accelerate the Healing of Wound

Biogenic Synthesis, Characterization, Along with Investigation of Cytotoxicity and Antibacterial Activities of Silver Nanoparticles Using Twigs of Polyalthia sclerophylla

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