Antimicrobial Activity of Protein Hydrolysate from Marine Molluscs Babylonia spirata (Linnaeus, 1758)

Amutha, K.; Ulagesan, Selvakumari

doi:10.7324/japs.2016.60711

Cited by 5 publications

(5 citation statements)

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“…Many bioactive compounds have been investigated predominantly for their antimicrobial, cytotoxic, anti-tumor and anti-inflammatory, antileukemic, antineoplastic and antiviral properties of mollusks [4][5][6][7]. Antibacterial and antiviral activities have been previously reported in the hemolymph of several molluscan species such as sea hares, sea slung, oysters, and mussels [8][9][10][11][12][13][14]. Over recent years, numerous studies on snail mucus composition have clarified many aspects of its properties, although much remains to be investigated on its antibacterial activity.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and Antifungal Activities of Proteins Extracted from Seven Different Snails

Ulagesan

Kim

2018

Applied Sciences

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Snails have been used both as a food and as a treatment for a variety of medicinal conditions. In this study, seven different snail proteins were evaluated for their antimicrobial activity. Fresh water and land snails of seven different live species were collected and identified. Crude proteins were extracted from seven different snails. The extracted proteins were estimated using Bradford's method and snail proteins were displayed using a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The seven different snail proteins were evaluated for their antimicrobial activity against various pathogenic bacterial and fungal cultures by agar well diffusion method and MIC (Minimum Inhibitory Concentration). One of the most active, crude proteins was from land snail Cryptozona bistrialis and its protein was capable of completely inhibiting the development of pathogenic bacterial and fungal cultures. This study shows that the land snail C. bistrialis proteins could be used as an antibiotic in biomedical research.

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Section: Introductionmentioning

confidence: 99%

Antibacterial and Antifungal Activities of Proteins Extracted from Seven Different Snails

Ulagesan

Kim

2018

Applied Sciences

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“…FBTP yielded the MIC and MBC values of 250 μg/mL and 500 μg/mL for P. ananatis and P. stewartii (Table 3). In previous studies, antibacterial activities have been described in several molluscan species, such as sea snails (Kuppusamy and Ulagesan, 2016), mussels (Romanenko et al, 2008) and oysters (Zasloff, 2019). The maximum antimicrobial activity of FBTP was observed against P. ananatis (30.011 ± 0.21%) and P. stewartii (31.981 ± 0.23) at 1000 µg/mL.…”

Section: Effective Inhibition Concentrationmentioning

confidence: 91%

“…The antimicrobial activity of the mollusc protein hydrolysate was determined by the agar well diffusion method (Kuppusamy and Ulagesan, 2016). LB agar was ISSN (print): 1823-8262, ISSN (online): 2231-7538 poured into the Petri plates and the inoculums were spread onto the agar with a sterile swab moistened with the bacterial suspension.…”

Section: Agar Well Diffusion Methodsmentioning

confidence: 99%

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The effect of lactic acid fermentation of Bactronophorus thoracites on antimicrobial activity against rice pathogens

Jamal¹,

Muhialdin²,

Saidi³

et al. 2022

MJM

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Aims: Leaf blight disease caused by Pantoea spp. reduces rice yields in numerous nations. However, the exact strategy to combat Pantoea spp. has yet to be determined. Bactronophorus thoracites is a promising source of natural antimicrobial agents due to their potential as a substrate to generate peptides with high antimicrobial activity. This study determined the effects of lactic acid fermentation using Lactobacillus casei ATCC334 as a starter culture on antimicrobial activity against rice pathogens, proximate composition, and amino acid profiles from B. thoracites crude extract. Methodology and results: Bactronophorus thoracites was washed and deshelled to collect the flesh and homogenised at 4 °C before freeze-drying. The freeze-dried samples were fermented with L. casei for 4 to 8 days at 37 °C. The antimicrobial activity, MIC and MBC were determined using a spectrometer. The fermented protein was subjected to proximate and amino acid analyses. The antimicrobial activity of fermented B. thoracites protein (FBTP) was significantly (p<0.05) decreased with the increased fermentation days (from 4 to 8 days). The antimicrobial activity was also increased when the glucose concentration increased from 2% to 3%. However, raising the glucose concentration to 4% decreased the antimicrobial activity. The antimicrobial activity was significantly (p<0.05) increased when the substratewater (S/W) ratio increased from 0.84% to 0.96%. The FBTP (4 days, 3% glucose concentration and 0.96% S/W ratio) showed high antimicrobial activity against Pantoea ananatis and P. stewartii. The MIC and MBC values for FBTP were 500 µg/mL and 250 µg/mL against P. ananatis and P. stewartii. The zones of inhibition value for FBTP were 16.0 ± 0.5 mm (1000 µg/mL) and 9.33 ± 0.57 mm (500 µg/mL) for P. ananatis, and 11.7 ± 0.61 mm (1000 µg/mL), 9.33 ± 0.58 mm (500 µg/mL) and 7.17 ± 0.77 mm (250 µg/mL) for P. stewartii. The proximate composition and amino acid profiles of the freeze-dried protein hydrolysate powder were characterised. FBTP produced a higher value of protein (61.56%) and ash (32.38%) and a lower value of total fat (0.273%) and carbohydrates (6.27%) than the B. thoracites crude extract. Total amino acid content was 39.480 g/100 g in B. thoracites crude extract and 155.442 g/100 g in FBTP. The essential amino acid glutamine was the most abundant in B. thoracites crude extract and methionine in FBTP. Conclusion, significance and impact of study:This study showed that lactic acid fermentation could produce FBTP using L. casei with improved functional characteristics and as a source of a natural antimicrobial agent against rice pathogens.

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“…Previous reports of the literature demonstrated that the benthic marine gastropods, especially those belonging to the family Babylonidae were endowed with nutritional and potential medicinal properties (Chakraborty & Salas, 2020; Govindarajalu, Anand, Chelladurai, & Kumaraguru, 2016; Kuppusamy & Ulagesan, 2016; Salas, Chakraborty, Sarada, & Vijayagopal, 2018). However, they were not subjected to extensive chemical investigation to characterize the bioactive leads (Altena & Gittenberger, 1981; Fraussen & Stratmann, 2013).…”

Section: Introductionmentioning

confidence: 99%

Antioxidative 2 H ‐chromenyls attenuate pro‐inflammatory 5‐lipoxygenase and carbolytic enzymes: Prospective bioactive agents from Babylonidae gastropod mollusk Babylonia spirata

Chakraborty

Salas

2020

J Food Biochem

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Oxygenated heterocycles are emerging as valuable pharmacophores involved in the prophylaxis and treatment of several diseases elicited by the reactive oxygen species. Bioassay‐led chromatographic fractionation of the organic extract of the gastropod mollusk Babylonia spirata (family Babylonidae) yielded two unprecedented 2H‐chromenyl derivatives characterized as 2‐(butyryloxy)‐5‐hydroxy‐hexahydro‐2H‐chromene‐3‐methyl carboxylate (1) and (3‐hydroxy‐hexahydro‐2H‐chromen‐2‐yl)methyl pentanoate (2). The chromenyl derivative (1) registered significantly greater attenuation potential against pro‐inflammatory 5‐lipoxygenase (IC50 ~ 2.02 mM) than those exhibited by the compound (2) (IC50 2.76 mM) and the non‐steroidal anti‐inflammatory drug ibuprofen (IC50 4.36 mM, p < .05). The compound (1) exhibited comparable antioxidant activity (IC50 1.47–1.72 mM) with standard antioxidative agent α‐tocopherol (IC50 1.4–1.7 mM). Inhibitory potential of chromenyl derivative (1) toward α‐glucosidase (IC50 1.18 mM) and α‐amylase (IC50 0.92 mM) was greater than those displayed by 2 (IC50 1.16–1.56 mM). Structure–activity relationships revealed that bioactivities of the compounds were determined by the electronic factors and hydrophilic–lipophilic balance. Practical applications The marine gastropod Babylonia spirata is one of the prominent edible gastropod species harvested from the coastlines along the southwestern region of the Indian peninsula. Two 2H‐chromenyl derivatives were isolated to homogeneity from the organic extract of the marine buccinid gastropod B. spirata by the bioactivity‐guided chromatographic fractionation and were found to possess potential antioxidant and attenuation properties against pro‐inflammatory 5‐lipoxygenase and carbolytic enzymes. The attenuation properties of the 2H‐chromenyls against pro‐inflammatory 5‐lipoxygenase showed that 2H‐chromenyl analogs possessed significantly greater anti‐inflammatory potential than the non‐steroidal anti‐inflammatory drug ibuprofen. In particular, the chromenyl derivative bearing 2H‐chromene‐3‐methyl carboxylate framework might constitute a prospective biogenic constituent in functional food and pharmaceutical applications for use against oxidative agents, including inflammation and hyperglycemic pathologies.

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Antimicrobial Activity of Protein Hydrolysate from Marine Molluscs Babylonia spirata (Linnaeus, 1758)

Cited by 5 publications

References 10 publications

Antibacterial and Antifungal Activities of Proteins Extracted from Seven Different Snails

Antibacterial and Antifungal Activities of Proteins Extracted from Seven Different Snails

The effect of lactic acid fermentation of Bactronophorus thoracites on antimicrobial activity against rice pathogens

Antioxidative 2 H ‐chromenyls attenuate pro‐inflammatory 5‐lipoxygenase and carbolytic enzymes: Prospective bioactive agents from Babylonidae gastropod mollusk Babylonia spirata

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