Antimicrobial Activity of Pleurotus squarrosulus on Clinical Pathogenic Bacteria and Fungi

Abstract

Aim:To evaluate the antimicrobial activities of Pleurotus squarrosulus mushroom extracts on bacterial and fungal isolates. Methodology: Pleurotus squarrosulus was extracted using ethanol, methanol and aqueous. Antimicrobial susceptibility tests were carried out by agar disc diffusion technique using National Committee of Clinical Laboratory Standard. Qualilative phytochemical analysis was carried out using standard methods. Results: Methanol, ethanol and aqueous extracts of Pleurotus squarrosulus were tested against E. coli, B. cereus, S. aureus, P. aeruginosa, C. albicans and C. glabrata. The different test microorganisms showed varied susceptibility to the test extracts. All the test organisms were Kalu and Kenneth; JAMB, 4(3): 1-9, 2017; Article no.JAMB.34644 2 inhibited by methanol, ethanol and aqueous extract at varied concentrations ranging between 500 mg/ml and 125 mg/ml. Statistically, inhibition of the antibacterial and antifungal control for the test organisms were significantly higher (P < 0.05) than that of the extracts. The phytochemical analysis revealed the presence of saponin, carbohydrates, tannins, flavonoids and proteins in all the extracts while glycoside and alkaloids, were found in some. Conclusion: The finding of this result suggest that Pleurotus squarrosulus possess broad-spectrum antimicrobial activity. The potential of developing antimicrobials from plants appear rewarding. Original Research Article

Antimicrobial activity of the edible mushroom Pleurotus eryngii (DC.) Quél grown in liquid medium

“…The results of this research are in accordance with those found by Subrata, Gunjan, Prakash, Mandal, and Krishnendu (2012), who verified antifungal activity against C. albicans using methanolic extracts of P. djamor, although no activity was noted for S. aureus, E. coli and P. aeruginosa. Extracts obtained using methanol, ethanol and water may also be favorable in antimicrobial tests, and may present satisfac tory results against E. coli, S. aureus, P. aeruginosa, C. albicans and C. glabrata (Kalu & Kenneth, 2017). The use of extracts makes it possible to perform antimicrobial tests, such as minimum inhibitory concentration, in addition to the isolation and purification of substances capable of inhibiting pathogenic microorganisms (Schillaci et al, 2013;Yehia & Al-Sheikh, 2014;Li & Shah, 2014;Finimundy et al, 2018;Musa et al, 2018).…”

Antibacterial Activity of Fomitopsis Betulina Cultural Liquid

“…lateritia, Rhodocollybia maculate, Russula fragilis, Scleroderma citrinum, Stereum hirsutum, Thelephora terrestris, Trametes hirsuta, Trichaptum fuscoviolaceum, Tubaria furfuracea were determined at concentration between 0.625 and 5 mg/ml [21]. In contrast, the tested samples of F. betulina cultural liquid inhibited the growth of P. aeruginosa АТСС 27853 significantly higher, than the methanol and ethanol extracts of Pluerotus squarrosulus fruit bodies with MBC at 62.5 mg/ml and 31.25 mg/ml, respectively [18]. We noted that results of the studies, available in the literature, are entirely focused on evaluation of the antibacterial properties of mushroom extracts in contrast to this work.…”

“…Samples of F. betulina cultural liquid, studied by us, possessed weaker activity against S. aureus ATCC 25923, but were active against P. aeruginosa АТСС 27853, compared to the ethanolic extracts of P. betulinus fruit bodies [12]. Different species of macrofungi extracts were also tested against these standard bacteria [18][19][20][21][22][23]. [21]; ethyl acetate (3.125 mg/ml) and acetone (12.5 mg/ml) extracts from Cordyceps taii mycelia [22]; methanol extract of Coriolus versicolor carpophores (5.0 mg/ml) [20]; methanol (62.5 mg/ml), ethanol and aqueous extracts (31.25 mg/ml) of Pluerotus squarrosulus carpophores [18]; methanolic extract of Cantharellus cibarius carpophores (20.0 mg/ml) [19].…”

“…Different species of macrofungi extracts were also tested against these standard bacteria [18][19][20][21][22][23]. [21]; ethyl acetate (3.125 mg/ml) and acetone (12.5 mg/ml) extracts from Cordyceps taii mycelia [22]; methanol extract of Coriolus versicolor carpophores (5.0 mg/ml) [20]; methanol (62.5 mg/ml), ethanol and aqueous extracts (31.25 mg/ml) of Pluerotus squarrosulus carpophores [18]; methanolic extract of Cantharellus cibarius carpophores (20.0 mg/ml) [19]. At the same time, the antimicrobial activity of F. betulina culture liquid against S. aureus ATCC 25923 was inferior to MBC of the chloroform extract (1.56 mg/ml) of Cordyceps taii mycelia [22], chloroform extract of Hygrophorus agathosmus (125 µg/ml), dichloromethane extract (>1000 µg/ml) of Suillus collitinus carpophores [23], and ethanol extract (0.625 mg/ml) of Hyphodontia paradoxa fruit body [21].…”

Antibacterial Potential of Protein Solution Separated from Bovine Serum by SDS Treatment

“…Studies have demonstrated the rapid development of Multi-Drug Resistant (MDR) bacterial strains which is a major global challenge in treating infectious diseases [1]. Several compounds from natural sources alternative to classical antibiotics combat the adverse effects [2,3]. Nevertheless, it is tiresome to identify and isolate the specific active compounds since they are highly complexed nature.…”