Antimicrobial Activity of Pantothenol against Staphylococci Possessing a Prokaryotic Type II Pantothenate Kinase

Chohnan, Shigeru; Murase, Misa; Kurikawa, Kota; Higashi, Kodai; Ogata, Yuta

doi:10.1264/jsme2.me13178

Cited by 10 publications

(5 citation statements)

References 21 publications

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“…Dexpanthenol has other properties making it ideal for use in the application being discussed here, specifically improvement during wound healing [45‐47]. It is also linked with reduction of oxidative stress [36], reduces Keratin Growth Factor (KGF) expression (important in growth and differentiation of the SC and barrier development) [48], has anti‐irritation properties [49] and antimicrobial activity against a range of skin bacteria including Staphylococcus aureus [50].…”

Section: Resultsmentioning

confidence: 99%

Targeted dry skin treatment using a multifunctional topical moisturizer

Stettler

Crowther²,

Brandt

et al. 2021

Intern J of Cosmetic Sci

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Objective The development of dry skin is a complex process, with a wide variety of factors each playing different roles in its evolution. Given this, it is important when designing a formulation to tackle dry skin that these varied aspects of skin behaviour are addressed. Presented here are the results of a 3‐week moisturization study carried out on dry legs. A wide range of traditional and more recently developed biophysical measurement methods have been combined with visual assessment of skin condition to enable multiple aspects of skin function to be determined. The observed changes in the skin are discussed in terms of the ingredients used in the moisturizing formulation. Methods A range of novel and traditional skin assessment methods and techniques were used to assess the effects of an oil in water‐based moisturizing product compared to an untreated site during a 3‐week in vivo study on dry lower leg skin. Results Statistically significant improvements were observed in a range of skin parameters as a result of product usage. Skin hydration assessed using Corneometer®, Epsilon® and visual dry skin grading all increased after 3 weeks of use. Skin barrier function measured using transepidermal water loss also improved. Levels of cholesterol, free fatty acids and Ceramide NH increased, as well as the average length of the stratum corneum (SC) lipid lamella bilayers, and the ratio of lipid to protein increased (measured using Lipbarvis® and in vivo Confocal Raman Spectroscopy). Increases in the levels of Ceramide EOS and NP were also observed, along with an improvement in corneocyte maturity, although these were not statistically significant. Conclusions Using a variety of traditional and novel skin assessment techniques, a wide range of factors associated with the evolution of dry skin have been assessed upon treatment with a new topical moisturizer. Product usage resulted in significant improvements to skin hydration and barrier function, the levels and morphology of SC barrier lipids, and overall epidermal differentiation. As a result there was a significant reduction in the characteristics associated with the development of dry skin after use of the test product.

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Section: Resultsmentioning

confidence: 99%

Targeted dry skin treatment using a multifunctional topical moisturizer

Stettler

Crowther²,

Brandt

et al. 2021

Intern J of Cosmetic Sci

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“…Pantothenate is involved in the Coenzyme A (CoA) synthesis, which functions as an acyl carrier and is a required cofactor for all living cells. Pantothenate analogs also have been shown to markedly suppress the growth of some opportunistic pathogens by inhibiting phosphorylation activity, which catalyzes the first step of the CoA biosynthetic pathway in specific bacteria, such as Staphylococcus species that inhabit human skin including Staphylococcus epidermidis, Staphylococcus saprophyticus, and Staphylococcus aureus ( 115 , 116 ), the most common microorganism identified in mastitis among lactating mothers ( 117 , 118 ), suggesting pantothenate to be effective in preventing infections by opportunistic pathogens. In our study, Staphylococcus aureus was not identified as DA by infant growth and was not correlated with maternal intake of vitamin B5, possibly due to our exclusion criteria of mastitis and sub-clinical mastitis which might have yielded low abundance of Staphylococcus aureus in our study population.…”

Section: Discussionmentioning

confidence: 99%

Human milk microbiome: associations with maternal diet and infant growth

Ajeeb,

Gonzalez,

Solomons

et al. 2024

Front. Nutr.

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IntroductionIngestion of human milk (HM) is identified as a significant factor associated with early infant gut microbial colonization, which has been associated with infant health and development. Maternal diet has been associated with the HM microbiome (HMM). However, a few studies have explored the associations among maternal diet, HMM, and infant growth during the first 6 months of lactation.MethodsFor this cross-sectional study, Mam-Mayan mother-infant dyads (n = 64) were recruited from 8 rural communities in the Western Highlands of Guatemala at two stages of lactation: early (6–46 days postpartum, n = 29) or late (109–184 days postpartum, n = 35). Recruited mothers had vaginally delivered singleton births, had no subclinical mastitis or antibiotic treatments, and breastfed their infants. Data collected at both stages of lactation included two 24-h recalls, milk samples, and infant growth status indicators: head-circumference-for-age-z-score (HCAZ), length-for-age-z-score (LAZ), and weight-for-age-z-score (WAZ). Infants were divided into subgroups: normal weight (WAZ ≥ −1SD) and mildly underweight (WAZ < −1SD), non-stunted (LAZ ≥ −1.5SD) and mildly stunted (LAZ < −1.5SD), and normal head-circumference (HCAZ ≥ −1SD) and smaller head-circumference (HCAZ < −1SD). HMM was identified using 16S rRNA gene sequencing; amplicon analysis was performed with the high-resolution ANCHOR pipeline, and DESeq2 identified the differentially abundant (DA) HMM at the species-level between infant growth groups (FDR < 0.05) in both early and late lactation.ResultsUsing both cluster and univariate analyses, we identified (a) positive correlations between infant growth clusters and maternal dietary clusters, (b) both positive and negative associations among maternal macronutrient and micronutrient intakes with the HMM at the species level and (c) distinct correlations between HMM DA taxa with maternal nutrient intakes and infant z-scores that differed between breast-fed infants experiencing growth faltering and normal growth in early and late lactation.ConclusionCollectively, these findings provide important evidence of the potential influence of maternal diet on the early-life growth of breastfed infants via modulation of the HMM.

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“…Both β-alanine and pantothenate promoted CAP resistance, verifying that the pantothenate pathway played an important role in CAP resistance. On the one hand, Pantothenol (pB5) is a provitamin of pantothenate, an inhibitor of the phosphorylation activity of the prokaryotic pantothenate kinase, which catalyzes the first step of the coenzyme A biosynthetic pathway ( Chohnan et al, 2014 ). Pantothenol decreased the percent survival rate, which showed that sensitivity to CAP increased when the pantothenate pathway was blocked.…”

Section: Discussionmentioning

confidence: 99%

Glutamate-pantothenate pathway promotes antibiotic resistance of Edwardsiella tarda

Yan,

Dong,

Wang

et al. 2023

Front. Microbiol.

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Although cellular metabolic states have been shown to modulate bacterial susceptibility to antibiotics, the interaction between glutamate (Glu) and chloramphenicol (CAP) resistance remains unclear because of the specificity of antibiotics and bacteria. We found that the level of Glu was upregulated in the CAP-resistant strain of Edwardsiella tarda according to a comparative metabolomics approach based on LC–MS/MS. Furthermore, we verified that exogenous metabolites related to Glu, the tricarboxylic acid (TCA) cycle, and glutathione (GSH) metabolism could promote CAP resistance in survival assays. If GSH metabolism or the TCA cycle is inhibited by L-buthionine sulfoximine or propanedioic acid, the promotion of CAP resistance by Glu in the corresponding pathway disappears. According to metabolomic analysis, exogenous Glu could change pantothenate metabolism, affecting GSH biosynthesis and the TCA cycle. These results showed that the glutamate-pantothenate pathway could promote CAP resistance by being involved in the synthesis of GSH, entering the TCA cycle by direct deamination, or indirectly affecting the metabolism of the two pathways by pantothenate. These results extend our knowledge of the effect of Glu on antibiotic resistance and suggest that the potential effect, which may aggravate antibiotic resistance, should be considered before Glu and GSH administration in the clinic.

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Antimicrobial Activity of Pantothenol against Staphylococci Possessing a Prokaryotic Type II Pantothenate Kinase

Cited by 10 publications

References 21 publications

Targeted dry skin treatment using a multifunctional topical moisturizer

Targeted dry skin treatment using a multifunctional topical moisturizer

Human milk microbiome: associations with maternal diet and infant growth

Glutamate-pantothenate pathway promotes antibiotic resistance of Edwardsiella tarda

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