Antimicrobial activity of nanoemulsion on cariogenic planktonic and biofilm organisms

Ramalingam, K.; Amaechi, Bennett T.; Ralph, Rawls H.; Lee, Valerie A.

doi:10.1016/j.archoralbio.2011.07.001

Cited by 62 publications

(43 citation statements)

References 33 publications

(40 reference statements)

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“…16,17 Recently, alternatives that might offer the possibility for efficient reduction of cariogenic microorganisms and, consequently, make a feasible control of dental caries are being sought. [18][19][20][21] Under these circumstances, photodynamic therapy (PDT) may be a possible new approach for caries treatment.…”

mentioning

confidence: 99%

Photodynamic Effects of Curcumin Against Cariogenic Pathogens

Araújo

Fontana

Bagnato

et al. 2012

Photomedicine and Laser Surgery

100

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Background data: The presence of Streptococcus mutans and Lactobacillus acidophilus in dental structure is an indicator of a cariogenic biofilm. Photodynamic therapy is a technique that involves the activation of photosensitizers by light in the presence of oxygen, resulting in the production of reactive radicals capable of inducing cell death. Reduction of bacteria levels can provide additional means of preventing dental caries. Objective: The present study evaluated the susceptibility of planktonic cultures of S. mutans (ATCC 25175) and L. acidophilus (ATCC-IAL-523) from the Adolfo Lutz Institute (IAL) to photodynamic therapy after sensitization with curcumin and exposure to blue light at 450 nm. Methods: Bacterial suspensions of S. mutans and L. acidophilus isolated (as single species) and combined (multspecies) were prepared and then evaluated. Four different groups were analyzed: L-D-(control group), L-D + (drug group), L + D-(light group), and L + D + (photodynamic therapy group). Two different concentrations of curcumin were tested (0.75 and 1.5 g/L) associated with a 5.7 J/cm 2 light emission diode. Results: Significant decreases ( p < 0.05) in the viability of S. mutans were only observed when the bacterial suspensions were exposed to both curcumin and light. Then, reductions in viability of up to 99.99% were observed when using 1.5 g/L of the photosensitizer. The susceptibility of L. acidophilus was considerably lower (21% and 37.6%) for both curcumin concentrations. Conclusions: Photodynamic therapy was found to be effective in reducing S. mutans and L. acidophilus on planktonic cultures. No significant reduction was found for L-D + , proving the absence of dark toxicity of the drug.

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confidence: 99%

Photodynamic Effects of Curcumin Against Cariogenic Pathogens

Araújo

Fontana

Bagnato

et al. 2012

Photomedicine and Laser Surgery

100

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“…In 2012, Ramalingam et al 168 evaluated the activity of CPC loaded onto a nanoemulsion against planktonic S. mutans, Lactobacillus casei, Actinomyces viscosus, C. albicans, and a mixed culture using microdilution technique and biofilm assay. The results demonstrated that compared to the components alone, the application of the formulation exhibited a better and enhanced activity against microorganisms, both individually and in mixed culture.…”

Section: Mes and Nanoemulsionsmentioning

confidence: 99%

Nanotechnology-based drug delivery systems for control of microbial biofilms: a review

Ramos¹,

Silva²,

Spósito³

et al. 2018

IJN

210

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Since the dawn of civilization, it has been understood that pathogenic microorganisms cause infectious conditions in humans, which at times, may prove fatal. Among the different virulent properties of microorganisms is their ability to form biofilms, which has been directly related to the development of chronic infections with increased disease severity. A problem in the elimination of such complex structures (biofilms) is resistance to the drugs that are currently used in clinical practice, and therefore, it becomes imperative to search for new compounds that have anti-biofilm activity. In this context, nanotechnology provides secure platforms for targeted delivery of drugs to treat numerous microbial infections that are caused by biofilms. Among the many applications of such nanotechnology-based drug delivery systems is their ability to enhance the bioactive potential of therapeutic agents. The present study reports the use of important nanoparticles, such as liposomes, microemulsions, cyclodextrins, solid lipid nanoparticles, polymeric nanoparticles, and metallic nanoparticles, in controlling microbial biofilms by targeted drug delivery. Such utilization of these nanosystems has led to a better understanding of their applications and their role in combating biofilms.

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“…The level of adhesion on glass surface was reduced by 94.2 to 99.5% in NE treated groups compared to positive (CHX) and negative controls. The antiadherence and anti-biofilm effects of NE suggest a promising anti-caries action [21].…”

Section: Differentmentioning

confidence: 99%

Commercial antimicrobials mouthrinses on caries and periodontitis-related biofilm control: a review of literature

Braga

Pires

Magalhães

2017

BDS

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This review aims to discuss the antimicrobial potential of different mouthrinses in respect to the control of dental caries and periodontal disease. The survey was conducted using PubMed and the following keywords: "antimicrobial agent " or "antiplaque agent," "dental biofilm" and "dental caries" or "periodontal disease" or "gingivitis". Only studies published in English, from 2011 to 2015, in journals with impact factor greater than 0.8, were selected. We found a total of 22 papers, 13 related to dental caries and 9 related to periodontal disease. Among the 13 studies involving cariogenic bacteria and/or biofilm, 6 were conducted in vitro, 3 in situ and 4 in vivo. Among 9 studies involving periodontal disease, 2 were in vitro and 7 in vivo. The main active agents tested were: CHX-Chlorhexidine, CPC-cetylpyridinium chloride and EO-Essential oils (alcohol/or alcohol-free). CHX was compared to EO in 6 studies, showing superiority in 3 studies, similarity in 1 study and inferiority in 2 studies. CPC has shown lower effect in plaque reduction compared to CHX and EO. There is still controversy about the effect of alcohol, but some studies have shown superiority for EO and CHX with alcohol on cariogenic and periodontopathogenic biofilms, respectively, when compared to alcohol-free version; for CPC, no difference was found. More clinical studies are needed for better understanding the mechanism of action and the differences in performance among the antiplaque agents.KeywordsAntimicrobial agents; Dental biofilm; Dental caries; Oral diseases; Periodontitis.

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Antimicrobial activity of nanoemulsion on cariogenic planktonic and biofilm organisms

Cited by 62 publications

References 33 publications

Photodynamic Effects of Curcumin Against Cariogenic Pathogens

Photodynamic Effects of Curcumin Against Cariogenic Pathogens

Nanotechnology-based drug delivery systems for control of microbial biofilms: a review

Commercial antimicrobials mouthrinses on caries and periodontitis-related biofilm control: a review of literature

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