Antimicrobial activity of linear lipopeptides derived from BP100 towards plant pathogens

Abstract

A collection of 36 lipopeptides were designed from the cecropin A-melittin hybrid peptide BP100 (H-Lys-Lys-Leu-Phe-Lys-Lys-Ile-Leu-Lys-Tyr-Leu-NH2) previously described with activity against phytopathogenic bacteria. These lipopeptides were synthesized on solid-phase and screened for their antimicrobial activity, toxicity and proteolytic stability. They incorporated a butanoyl, a hexanoyl or a lauroyl group at the N-terminus or at the side chain of a lysine residue placed at each position of the sequence. Their antimicrobial activity and hemolysis depended on the fatty acid length and its position. In particular, lipopeptides containing a butanoyl or a hexanoyl chain exhibited the best biological activity profile. In addition, we observed that the incorporation of the acyl group did not induce the overexpression of defense-related genes in tomato. Best lipopeptides were BP370, BP378, BP381, BP387 and BP389, which were highly active against all the pathogens tested (minimum inhibitory concentration of 0.8 to 12.5 μM), low hemolytic, low phytotoxic and significantly stable to protease degradation. This family of lipopeptides might be promising functional peptides useful for plant protection.

Targeted Amino Acid Substitutions in a Trichoderma Peptaibol Confer Activity against Fungal Plant Pathogens and Protect Host Tissues from Botrytis cinerea Infection

“…Some of the analogs synthesized and tested in the present work were chosen in light of the promising results obtained for them in previous studies involving human bacterial pathogens [ 30 , 32 ]. Cationic peptides have been previously found to be effective against fungal plant pathogens [ 33 , 34 ]. In the present work, the poor reactivity of Aib residues has been successfully overcome using a green [ 35 ] and cost-effective synthetic strategy to produce trichogin analogs.…”

Lesson from Ecotoxicity: Revisiting the Microbial Lipopeptides for the Management of Emerging Diseases for Crop Protection

“…These three genera are considered as factories for the production of biologically active molecules which are potent growth inhibitors of phytopathogens. Lipopeptides, a subclass of antimicrobial peptides, arenow emerging as an attractive alternative for the development of new peptide-based biopesticides [84,200]. The usefulness of lipopeptides is being evaluated as a potent versatile weapon for the control of a wide range of phytopathogens.…”

A Synergic Potential of Antimicrobial Peptides against Pseudomonas syringae pv. actinidiae

“…This confluence of factors makes research into novel efficient and sustainable strategies a priority to control Psa. Among the several innovative strategies that have been proposed as short-term alternatives to control Psa [ 27 , 28 , 29 , 30 ], namely the use of bacteriophages [ 31 ], essential oils, and plants’ metabolites [ 32 , 33 , 34 ], the antimicrobial peptides (AMPs) are probably the most consensual solution towards a more sustainable and safer agriculture [ 32 , 33 , 34 ].…”

“…BP100 is one of the most tested AMPs, since Badosa et al [ 58 ] designed and demonstrated its high potential to control phytopathogenic bacteria. The bioactivity of this AMP has been demonstrated against Erwinia amylovora [ 48 ], Xyllela fastidiosa [ 51 ], Dickeya chrysanthemi [ 59 ] and also tested against Psa [ 29 ]. Due to the potential of this peptide, several analogs were designed and tested on pathogenic bacteria, such as RW-BP100 [ 60 ].…”