Antimicrobial Activity of Lactobacillus Isolated From Kashk-e Zard and Tarkhineh, Two Iranian Traditional Fermented Foods

Mashak, Zohreh

doi:10.17795/ijep34692

Cited by 8 publications

(8 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All strains showed strong to moderate antimicrobial activity against S. aureus, Salmonella Typhimurium and E. coli. L. acidophilus, L. plantarum and L. rhamnosus showed moderate antimicrobial activity against E. coli while other researchers reported a relatively higher antimicrobial activity of Lactobacillus species against E. coli [41]. Our results are in comparison with the finding, which showed moderate activity against Salmonella species [35].…”

Section: Discussionsupporting

confidence: 63%

Antimicrobial role of Lactobacillus species as potential probiotics against enteropathogenic bacteria in chickens

Ahmed

Vohra

Khan

et al. 2019

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: The emergence of antimicrobial resistance among bacterial community resulted in a ban on drugs as the growth promoter in poultry feed. This situation demands to explore alternatives as food supplements with health benefit to poultry. Therefore, probiotic microorganisms, which are considered as safe and possess various health benefits can be a choice. Present study was designed to explore the probiotic potential of the isolated lactobacillus species in chickens. Methodology: Out of 220 samples, 100 Lactobacillus species were isolated from various regions of chicken intestine. They were further characterized on the basis of morphology, staining and catalase test. Species-level identification was made by amplifying Lactobacillus specific 16S rRNA gene. Out of 100 isolates, 21 were selected for sequencing on the basis of band intensity. Results: Among 21 sequences, 16 were identified as L. paracasei (n = 6), L. salivarius (n = 3), L. johnsonii (n = 3), and L. agilis, L. fermentum, L. sakei, and L. curvatus (n = 1 each). These strains were found to be significantly acid-tolerant with 81.68 - 85.01% survival rate at pH 2)and bile-tolerant with 81.96 -84.65% survival rate at 0.3% bile. Except three; all strains showed salt tolerance to 2% and 4% NaCl. Among 21 Lactobacillus strains, 6 showed good antimicrobial activities against S. aureus, Salmonella Typhimurium and E. coli. Conclusion: Lactobacillus species with probiotic property can be used in poultry feed formulation for their health benefit to combat gastrointestinal infections.

show abstract

Section: Discussionsupporting

confidence: 63%

Antimicrobial role of Lactobacillus species as potential probiotics against enteropathogenic bacteria in chickens

Ahmed

Vohra

Khan

et al. 2019

J Infect Dev Ctries

View full text Add to dashboard Cite

show abstract

“…The Lactobacillus isolates ( L. plantarum , L. fermentum and L. salivarius ) from fermented fruits and vegetables had broad antibacterial spectrum (ZDI: 26–28 mm), against food-borne bacterial pathogens, as has been reported by Manzoor et al [ 40 ]. A large number of lactobacilli isolates, including L. acidophilus , L. plantarum and L. rhamnosus , procured from traditional fermented foods prepared with the combination of cereal and dairy materials, had excellent antibacterial activity against E. coli with ZDIs 17–20, 21–30, 14–31 mm, respectively [ 10 ].The probiotic Lactobacillus strains: L. fermentum , L. casei and L. acidophilus , isolated from buffalo milk showed growth inhibitory activity against Vibrio cholerae , S. typhi , E. coli , and Shigella species having ZDIs 10–22 mm [ 36 ]. The lactobacilli strains, including L. rhamnosus and L. plantarum , procured from dairy food products (commercially available yoghurt and cheese) and rumen contents of cow did not show growth inhibitory activity against E. coli , while Salmonella menston was found sensitive to all the test lactobacilli, as per the demonstration of Jose et al [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

“…It has been demonstrated that the Lactobacillus plantarum isolates could protect the infection caused with Salmonella typhi through interference with growth as well as virulence properties (adherence, invasion, and cytotoxicity) of the pathogen [ 7 ]. A large number of lactobacilli isolates, procured from traditional fermented foods prepared with the combination of cereals and dairy materials, including L. acidophilus , L. plantarum and L. rhamnosus had excellent antibacterial activity against Escherichia coli ATCC 700728 standard strain [ 10 ]. The lactic acid bacterial strains L. acidophilus , L. plantarum , L. fermentum , L. casei and L. rhamnosus , can effectively be applied against the urinary tract infection causing Proteus species ( P. mirabilis and P. vulgaris ), as has been reported by Goudarzi et al [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Indigenous Probiotic Lactobacillus Isolates Presenting Antibiotic like Activity against Human Pathogenic Bacteria

et al. 2017

View full text Add to dashboard Cite

Background: Indigenous lactic acid bacteria are well known probiotics having antibacterial activity against potentially pathogenic bacteria. This study aims to characterize the curd lactobacilli for their probiotic potentiality and antagonistic activity against clinical bacteria. Methods: Four curd samples were processed microbiologically for the isolation of lactic acid bacteria (LAB). The LAB strains obtained were identified by conventional methods: cultural aspect, gram-staining, biochemical and sugar fermentation tests. The probiotic properties were justified with tolerance to low-pH, bile salt and sodium chloride, and the antagonistic activity of the lactobacilli against human pathogenic bacteria (Escherichia coli, Proteus vulgaris, Acinetobacter baumannii and Salmonella enterica serovar Typhi) was assessed. Hemolytic activity and antibiotic susceptibility were determined for the lactobacilli isolates, and the cumulative probiotic potential (CPP) values were recorded. Result: Four lactobacilli isolates, L. animalis LMEM6, L. plantarum LMEM7, L. acidophilus LMEM8 and L. rhamnosus LMEM9, procured from the curd samples, survived in low-pH and high bile salt conditions, and showed growth inhibitory activity against the indicator bacteria by agar-well (zone diameter of inhibition; ZDIs: 13.67 ± 0.58–29.50 ± 2.10 mm) and agar overlay (ZDIs: 11.33 ± 0.58–35.67 ± 2.52 mm) methods; the average growth inhibitory activity of lactobacilli ranged 233.34 ± 45.54–280.56 ± 83.67 AU/mL, against the test bacterial pathogens. All the lactobacilli were non-hemolytic and sensitive to most of the test antibiotics. The CPP values of the isolated LAB were recorded as 80–100%. Conclusion: The curd lactobacilli procured might be used as the valid candidates of probiotics, and bio-therapeutics against bacterial infection to humans.

show abstract

“…The probiotic Lactobacillus strains: L. fermentum, L. casei and L. acidophilus, isolated from buffalo milk showed growth inhibitory activity against Vibrio cholerae, S. typhi, E. coli, and Shigella species having ZDIs 10-22 mm (Rahman et al, 2015). Many lactobacilli isolates, including L. plantarum obtained from traditional fermented foods prepared with the combination of cereal and dairy materials, had an excellent antibacterial activity against E. coli with ZDIs 20 mm (Mashak et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Secuenciación y evaluación del efecto antimicrobiano de bacterias acidolácticas frente a cepas de Salmonella Tiphymurium

et al. 2019

View full text Add to dashboard Cite

La producción intensiva y extensiva de cerdo en Argentina y España respectivamente presenta factores productivos diferentes. Sin embargo, cuando se trata de amenazas ante patógenos, específicamente Salmonella, ambos se ven afectados. Salmonella es un patógeno común en los alimentos relacionado con las toxiinfecciones alimentarias que provoca graves problemas en la salud pública. Las bacterias acidolácticas, específicamente los géneros Lactobacillus y Pediococcus se han caracterizado en los últimos años por tener gran importancia como antagonistas de diferentes bacterias patógenas. Una alternativa de control es la aplicación de Lactobacillus a lo largo de la cadena productiva de cerdo, evitando así la llegada de este patógeno a los consumidores. Se realizó una evaluación preliminar de la capacidad antagónica de bacterias acidolácticas identificadas tras secuenciación como Lactobacillus plantarum y Pediococcus acidilactici ante Salmonella Tiphymurium aislada de producción intensiva de cerdo de Argentina y Salmonella Tiphymurium aislada de producción extensiva de cerdo en España. Los resultados obtenidos reflejaron una alta capacidad antimicrobiana de las BAL seleccionadas ante ambas cepas patógenas (93.75%). De esta manera, se proponen como potenciales bacterias biopreservadoras en la producción de carne de cerdo y subproductos.

show abstract

Antimicrobial Activity of Lactobacillus Isolated From Kashk-e Zard and Tarkhineh, Two Iranian Traditional Fermented Foods

Abstract: Background: Nowadays, microbial food safety is an increasing public health concern worldwide, especially in developing countries.

Cited by 8 publications

References 38 publications

Antimicrobial role of Lactobacillus species as potential probiotics against enteropathogenic bacteria in chickens

Antimicrobial role of Lactobacillus species as potential probiotics against enteropathogenic bacteria in chickens

Indigenous Probiotic Lactobacillus Isolates Presenting Antibiotic like Activity against Human Pathogenic Bacteria

Secuenciación y evaluación del efecto antimicrobiano de bacterias acidolácticas frente a cepas de Salmonella Tiphymurium

Contact Info

Product

Resources

About