Antimicrobial activity of Lactobacillus fermentum TcUESC01 against Streptococcus mutans UA159

Rodrigues, Jeisa Zielle de Souza; Passos, Manuela Ribeiro; Neres, Nayara Silva de Macêdo; Almeida, Rafael Silva; Pita, Louise Soares; Santos, Iago Almeida; Silveira, Paulo Henrique Santana; Reis, Mariane Mares; Santos, Isabella Porto; Ricardo, Luccas de Oliveira Negrão; Lima, Brenda Oliveira; Marinho, Patrick D'Orleans Farias; Soares, Ananda Brito; Andrade, Leonardo Oliveira Silva Bastos; Pessoa, Stela Mares Brasileiro; Silva, Marlon Mário Leles; Oliveira, Milena Cardoso; Silva, Jamile Pinheiro da; Moura, Mariana Araújo; Cruz, Manoel Jerônimo Moreira; Marques, Lucas Miranda; Santos, Tizá Teles; Pires, Polyane Novais; Dias, João Carlos Teixeira; Rezende, Rachel Passos; Uetanabaro, Ana Paula Trovatti; Yatsuda, Regiane

doi:10.1016/j.micpath.2020.104063

Cited by 26 publications

(17 citation statements)

References 39 publications

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“…L. casei , L. plantarum , L. rhamnosus , L. salivarius, L. fermentum , and L. paracasei were found to exert the greatest antimicrobial activity against S. mutans ( Koll-Klais et al, 2005 ; Teanpaisan et al, 2011 ). L. fermentum supernatant revealed the presence of antimicrobial compounds mainly organic acids, hydrogen peroxides and bacteriocins ( Rodrigues et al, 2020 ). Phosphoric acid was produced by L. fermentum and it has strong antimicrobial properties against S. mutans ( Prado et al, 2015 ), the bacteriocin detected in culture supernatant showed a broad spectrum of antimicrobial activity against gram positive bacteria such as S. mutans by formation of pores in the cytoplasmic membrane resulting in energy loss and alteration in the activity of important enzymes in the target bacteria ( Mokoena, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

High Counts and Anthracene Degradation Ability of Streptococcus mutans and Veillonella parvula Isolated From the Oral Cavity of Cigarette Smokers and Non-smokers

et al. 2021

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The composition and metabolic functions of oral microbiota are affected by many factors including smoking leading to several health problems. Cigarette smoking is associated with changes in oral microbiota composition and function. However, it is not known if the depletion of certain bacterial genera and species is due to specific toxins in cigarette smoke, or indirectly due to competition for colonization with smoking-enriched bacteria. Therefore, the aim of this study was to determine the effect of cigarette smoking on the microbial prevalence and polycyclic aromatic hydrocarbons (PAHs) biodegradation of selected enriched and depleted oral bacteria from oral microbiota of smokers compared to that in non-smokers. Samples of oral rinse from smokers and non-smokers were collected (n = 23, 12 smokers and 11 non-smokers) and screened for oral bacterial strains of Streptococcus mutans, Lactobacillus spp., and Veillonella spp. Comparing counts, S. mutans, V. tobetsuensis, and V. dispar showed higher counts in smokers compared to non-smokers while the Lactobacillus spp. were higher in non-smokers. Lactobacillus fermentum was prevalent in smokers, representing 91.67% of the total Lactobacillus spp. isolates. The biodegradation potential of anthracene; a representative of PAHs of collected isolates, in single and mixed cultures, was assayed with anthracene as the sole source of carbon using 2,6-dichlorophenol indophenol (2,6-DCPIP) as indicator. S. mutans isolates recovered from smokers showed higher degradation of anthracene compared to those recovered from non-smokers. The anaerobic anthracene biodegradation activity of V. parvula isolates from non-smokers was the highest among all isolates of the three recovered genera from the same subject. The anthracene biodegradation potential of Lactobacillus spp. was variable. Combinations of isolated bacteria in co-cultures showed that Lactobacillus spp. interfered with anthracene biodegradation ability along with the viable counts of S. mutans and Veillonella spp. In conclusion, oral dysbiosis due to cigarette smoking was observed not only due to changes in oral bacterial relative abundance but also extended to bacterial functions such as anthracene biodegradation tested in this study. Microbe–microbe interactions changed the anthracene biodegradation potential and growth of the microbial mixture compared to their corresponding single isolates, and these changes differ according to the constituting bacteria.

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Section: Discussionmentioning

confidence: 99%

High Counts and Anthracene Degradation Ability of Streptococcus mutans and Veillonella parvula Isolated From the Oral Cavity of Cigarette Smokers and Non-smokers

et al. 2021

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“…The presence of diffusible inhibitory substances was also evaluated by a microdiffusion assay on semi-solid agar adapted from Rodrigues et al [ 66 ]. Initially, suspensions of pathogens (10 8 CFU mL -1 ) were added (1%, v/v) on semi-solid BHI agar (0.75%, w/v) and plated.…”

Section: Methodsmentioning

confidence: 99%

“…The CFCS metabolome was analysed by gas chromatography-mass spectrometry (GC-MS) according to the method described by Rodrigues et al [ 66 ]. Initially, the CFCS of each Lactobacillus strain was previously conditioned (48 h, -18°C), then the frozen samples were inserted in a freeze dryer (Alpha 1-2 LDplus, CHRIST, Osterode, Germany) and subjected to the sublimation drying process (48 h) in two distinct phases.…”

Section: Methodsmentioning

confidence: 99%

Lactiplantibacillus plantarum strains isolated from spontaneously fermented cocoa exhibit potential probiotic properties against Gardnerella vaginalis and Neisseria gonorrhoeae

et al. 2021

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Background Probiotics are important tools in therapies against vaginal infections and can assist traditional antibiotic therapies in restoring healthy microbiota. Recent research has shown that microorganisms belonging to the genus Lactobacillus have probiotic potential. Thus, this study evaluated the potential in vitro probiotic properties of three strains of Lactiplantibacillus plantarum, isolated during the fermentation of high-quality cocoa, against Gardnerella vaginalis and Neisseria gonorrhoeae. Strains were evaluated for their physiological, safety, and antimicrobial characteristics. Results The hydrophobicity of L. plantarum strains varied from 26.67 to 91.67%, and their autoaggregation varied from 18.10 to 30.64%. The co-aggregation of L. plantarum strains with G. vaginalis ranged from 14.73 to 16.31%, and from 29.14 to 45.76% with N. gonorrhoeae. All L. plantarum strains could moderately or strongly produce biofilms. L. plantarum strains did not show haemolytic activity and were generally sensitive to the tested antimicrobials. All lactobacillus strains were tolerant to heat and pH resistance tests. All three strains of L. plantarum showed antimicrobial activity against the tested pathogens. The coincubation of L. plantarum strains with pathogens showed that the culture pH remained below 4.5 after 24 h. All cell-free culture supernatants (CFCS) demonstrated activity against the two pathogens tested, and all L. plantarum strains produced hydrogen peroxide. CFCS characterisation in conjunction with gas chromatography revealed that organic acids, especially lactic acid, were responsible for the antimicrobial activity against the pathogens evaluated. Conclusion The three strains of L. plantarum presented significant probiotic characteristics against the two pathogens of clinical importance. In vitro screening identified strong probiotic candidates for in vivo studies for the treatment of vaginal infections.

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“…The antimicrobial activity of lactobacilli against pathogens was tested by coculture assay adapted from Hütt et al [ 30 ]. Initially, activated cultures of pathogens and lactobacilli (10 8 CFU.mL −1 ) were inoculated together (1%, v / v ) in mixed growth medium (0.5 mL BHI broth + 0.5 mL MRS broth) and were incubated overnight.…”

Section: Methodsmentioning

confidence: 99%

Gardnerella vaginalis and Neisseria gonorrhoeae Are Effectively Inhibited by Lactobacilli with Probiotic Properties Isolated from Brazilian Cupuaçu (Theobroma grandiflorum) Fruit

Selis

Oliveira

Anjos

et al. 2021

BioMed Research International

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In recent years, certain Lactobacillus sp. have emerged in health care as an alternative therapy for various diseases. Based on this, this study is aimed at evaluating in vitro the potential probiotics of five lactobacilli strains isolated from pulp of cupuaçu fruit fermentation against Gardnerella vaginalis and Neisseria gonorrhoeae. Our lactobacilli strains were classified as safe for use in humans, and they were tolerant to heat and pH. Our strains were biofilm producers, while hydrophobicity and autoaggregation varied from 13% to 86% and 13% to 25%, respectively. The coaggregation of lactobacilli used in this study with G. vaginalis and N. gonorrhoeae ranged from 15% to 36% and 32% to 52%, respectively. Antimicrobial activity was present in all tested Lactobacillus strains against both pathogens, and the growth of pathogens in coculture was reduced by the presence of our lactobacilli. Also, all tested lactobacilli reduced the pH of the culture, even in incubation with pathogens after 24 hours. The cell-free culture supernatants (CFCS) of all five lactobacilli demonstrated activity against the two pathogens with a halo presence and CFCS characterization assay together with gas chromatography revealed that lactic acid was the most abundant organic acid in the samples (50% to 62%). Our results demonstrated that the organic acid production profile is strain-specific. This study revealed that cupuaçu is a promising source of microorganisms with probiotic properties against genital pathogens. We demonstrated by in vitro tests that our Lactobacillus strains have probiotic properties. However, the absence of in vivo tests is a limitation of our work due to the need to evaluate the interaction of our lactobacilli with pathogens in the vaginal mucosa. We believe that these findings may be useful in developing a product containing our lactobacilli and their supernatants in order to support with vaginal health.

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Antimicrobial activity of Lactobacillus fermentum TcUESC01 against Streptococcus mutans UA159

Cited by 26 publications

References 39 publications

High Counts and Anthracene Degradation Ability of Streptococcus mutans and Veillonella parvula Isolated From the Oral Cavity of Cigarette Smokers and Non-smokers

High Counts and Anthracene Degradation Ability of Streptococcus mutans and Veillonella parvula Isolated From the Oral Cavity of Cigarette Smokers and Non-smokers

Lactiplantibacillus plantarum strains isolated from spontaneously fermented cocoa exhibit potential probiotic properties against Gardnerella vaginalis and Neisseria gonorrhoeae

Gardnerella vaginalis and Neisseria gonorrhoeae Are Effectively Inhibited by Lactobacilli with Probiotic Properties Isolated from Brazilian Cupuaçu (Theobroma grandiflorum) Fruit

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