Ixora coccinea (Rubiaceae family) has been used traditionally for a variety aliments and also cultivated for ornamental purposes. The present study was conducted to evaluate the antibacterial activity of methanol and aqueous extracts of flower of Ixora coccinea against six fish virulence strains of bacterial isolates viz., Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Vibrio cholerae, Aeromonas hydrophila and Pseudomonas aeruginosa. The antibacterial potential of Ixora coccinea methanol extract was tested by using Agar well diffusion method. The methanol extract of Ixora coccinea showed maximum zone of inhibition when compared to aqueous extract. Phytochemical tests were performed from the both extracts and showed that the antibacterial activity of Ixora coccinea was due to the presence of Phytochemical compound like alkaloids, tripenoids, saponins, flavonoids, phenols, tannins, protein, carbohydrates and glycosides. Ixora coccinea methanol flower extracts was analyzed by using the HPTLC.