Antimicrobial Activity of Intrinsic Antimicrobial Polymers Based on Poly((tertbutyl-amino)-methyl-styrene) Against Selected Pathogenic and Spoilage Microorganisms Relevant in Meat Processing Facilities

Braun, Carina; Dohlen, Sophia; Ilg, Yvonne; Brodkorb, Florian; Fische, Björn; Heindir, Patrick; Kalbfleisch, Katrin; Richter, Thomas; Robers, Oliver; Kreyenschmidt, Martin; Lorenz, Reinhard; Kreyenschmidt, Judith

doi:10.4172/2472-1212.1000136

Cited by 4 publications

(4 citation statements)

References 37 publications

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“…As an example, in different studies, a higher activity of high molecular weight chitosan against Gram-positive bacteria ( S. aureus ) compared with Gram-negative bacteria, E. coli and Ps. fluorescens was observed [36,37,39]. In our results, P. fluorescens is even more resistant than E. coli , which is due to higher production of exopolysaccharides by P. fluorescens .…”

Section: Resultssupporting

confidence: 48%

“…If log10-reduction Ø ≥ 2 log10 units is reached, the antimicrobial activity against B. thermosphacta and P. fluorescens is then tested. The test will be conducted at a constant temperature of 7 °C for 24 h. In case the Ø log10-reduction is ≥ 2 log10, highly concentrated meat extract solution (18 μg mL −1 ) will be chosen as the reference media for perishable foods [36,37,38,39,40].…”

Section: Methodsmentioning

confidence: 99%

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Antimicrobial Activity of Lignin and Lignin-Derived Cellulose and Chitosan Composites against Selected Pathogenic and Spoilage Microorganisms

et al. 2019

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The antiradical and antimicrobial activity of lignin and lignin-based films are both of great interest for applications such as food packaging additives. The polyphenolic structure of lignin in addition to the presence of O-containing functional groups is potentially responsible for these activities. This study used DPPH assays to discuss the antiradical activity of HPMC/lignin and HPMC/lignin/chitosan films. The scavenging activity (SA) of both binary (HPMC/lignin) and ternary (HPMC/lignin/chitosan) systems was affected by the percentage of the added lignin: the 5% addition showed the highest activity and the 30% addition had the lowest. Both scavenging activity and antimicrobial activity are dependent on the biomass source showing the following trend: organosolv of softwood > kraft of softwood > organosolv of grass. Testing the antimicrobial activities of lignins and lignin-containing films showed high antimicrobial activities against Gram-positive and Gram-negative bacteria at 35 °C and at low temperatures (0–7 °C). Purification of kraft lignin has a negative effect on the antimicrobial activity while storage has positive effect. The lignin release in the produced films affected the activity positively and the chitosan addition enhances the activity even more for both Gram-positive and Gram-negative bacteria. Testing the films against spoilage bacteria that grow at low temperatures revealed the activity of the 30% addition on HPMC/L1 film against both B. thermosphacta and P. fluorescens while L5 was active only against B. thermosphacta. In HPMC/lignin/chitosan films, the 5% addition exhibited activity against both B. thermosphacta and P. fluorescens.

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Section: Resultssupporting

confidence: 48%

Section: Methodsmentioning

confidence: 99%

Antimicrobial Activity of Lignin and Lignin-Derived Cellulose and Chitosan Composites against Selected Pathogenic and Spoilage Microorganisms

et al. 2019

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“…Further studies are required to clarify the correlation of concentration and antimicrobial activity. Studies of the antimicrobial activity were performed following procedures reported to investigate intrinsically antimicrobial polymers based on poly((tertbutyl-amino)-methyl-styrene) [62][63][64][65][66] and coatings based on HPMC/lignin/chitosan [52]. Two different nutritions were used: sodium chloride (NaCl) and physiological saline solution (NB) of different concentrations (Figure 2).…”

Section: Antibacterial Activity Of Kraft Ligninmentioning

confidence: 99%

Antimicrobial Activity of Lignin-Derived Polyurethane Coatings Prepared from Unmodified and Demethylated Lignins

et al. 2019

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Due to global ecological and economic challenges that have been correlated to the transition from fossil-based to renewable resources, fundamental studies are being performed worldwide to replace fossil fuel raw materials in plastic production. One aspect of current research is the development of lignin-derived polyols to substitute expensive fossil-based polyol components for polyurethane and polyester production. This article describes the synthesis of bioactive lignin-based polyurethane coatings using unmodified and demethylated Kraft lignins. Demethylation was performed to enhance the reaction selectivity toward polyurethane formation. The antimicrobial activity was tested according to a slightly modified standard test (JIS Z 2801(JIS Z :2010. Besides effects caused by the lignins themselves, triphenylmethane derivatives (brilliant green and crystal violet) were used as additional antimicrobial substances. Results showed increased antimicrobial capacity against Staphylococcus aureus. Furthermore, the coating color could be varied from dark brown to green and blue, respectively.Coatings 2019, 9, 494 2 of 16 and DNA in cells of mice against oxidation damage [19] have been tested. Gao [20] and Bshena [21] studied the antimicrobial activity of various textiles, using lignin incorporated into polyethylene films and applied in the finishing processes. For textiles, there are special requirements such as non-toxicity to the consumer, namely cytotoxicity, allergy or irritation and sensitization. In other recent studies, lignosulfonic acid is reported to exhibit broad-spectrum anti-HIV (human immunodeficiency virus) and anti-HSV (herpes simplex virus) properties [22,23]. Thus, Qiu investigated the anti-HIV-1 activity-potential of lignosulfonates as a microbicide to prevent HIV-1 sexual transmission [23]. Another recently reported study revealed that the antimicrobial capacity of lignin correlates with the phenolic components, specifically the side chain structure and the nature of further functional groups [24]. Typically, the presence of a double bond in α, β positions of the side chain and a methyl group in the γ position grants the phenolic fragments with the most potency against microorganisms. However, none of the hitherto published studies included the investigation of the antibacterial activity of lignin when included in polymeric matrices.Unmodified lignin is widely studied as a component for polymer production with a focus on phenol-formaldehyde resins and polyurethanes (PUs) [25], where lignin is used as polyol substitute due to the high amount of hydroxyl groups resulting in high crosslinking densities and variable mechanical properties [26][27][28]. In previous studies, lignin-derived polyurethane coatings have been prepared using Kraft lignin isolated at room temperature from aqueous media (black liquor) at different pH values [29]. In addition, their antioxidative activity has been investigated using the Folin-Ciocalteu (FC) assay [30]. Although lignin contains many functionalities, they are often diff...

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“…Nisin and sodium nitrite (final concentration of 11.2 mg/mL) were used as food preservative controls, and bacterial inoculum without treatment was used as the untreated growth control (UNC). Bacterial counts were reported as log 10 CFU/mL, and log 10 reductions at minute 180 were defined as the difference between the logarithm of the bacterial count on the UNC and the treated control (Hüwe et al., 2017): Bacterial reduction ( t = 180 min) = [log 10 (bacteria with peptide or food preservative treatment, t = 180 min) ] − [log 10 (bacteria without treatment, t = 180 min) ].…”

Section: Methodsmentioning

confidence: 99%

Antibacterial properties of peptides from chia (Salvia hispanica L.) applied to pork meat preservation

León Madrazo,

Segura Campos

2023

Journal of Food Science

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Chia‐derived peptides might represent a novel alternative to conventional preservatives in food. Despite the antibacterial potential of these molecules, their food application is still limited. This study aimed to evaluate chia‐derived peptides' antibacterial and antibiofilm potential in food preservation. The peptides YACLKVK, KLKKNL, KLLKKYL, and KKLLKI were synthesized, and their antibacterial activity against Listeria monocytogenes, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Salmonella Enteritidis was evaluated through microdilution tests. A bacterial killing kinetic assay determined bacterial growth over time. The ability to prevent and eradicate S. aureus biofilm was assessed by crystal violet staining. The hemolytic and cytotoxic activities were determined in human red blood cells and fibroblasts using free hemoglobin detection and (3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide) assays, respectively. Finally, a microbial challenge was performed on meat samples inoculated with L. monocytogenes and S. Enteritidis to determine their inhibitory effects on pork meat. Results showed the potential antibacterial activity of these peptides, with minimum inhibitory concentrations ranging from 0.23 to 5.58 mg/mL. Biofilm inhibition percentages were above 40%, and eradication percentages were lower than 20%. In vitro assays in human red blood cells and fibroblasts demonstrated that peptides are not hemolytic or cytotoxic agents. In microbiological challenge testing, KKLLKI showed the most promising antibacterial effects against S. Enteritidis on refrigerated pork meat samples. These findings suggest that chia‐derived peptides have the potential as natural food preservatives due to their antibacterial and antibiofilm properties. Notably, KKLLKI demonstrated promising antibacterial effects against Salmonella spp. on a complex food matrix, such as pork meat.Practical ApplicationChia‐derived peptides can be a safer alternative to synthetic preservatives in the food industry because the latter may be detrimental to human health. Salmonella spp. growth on chilled pork meat was shown to be inhibited by the peptide KKLLKI, indicating that the use of these peptides may offer a more secure and natural alternative to synthetic preservatives.

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Antimicrobial Activity of Intrinsic Antimicrobial Polymers Based on Poly((tertbutyl-amino)-methyl-styrene) Against Selected Pathogenic and Spoilage Microorganisms Relevant in Meat Processing Facilities

Cited by 4 publications

References 37 publications

Antimicrobial Activity of Lignin and Lignin-Derived Cellulose and Chitosan Composites against Selected Pathogenic and Spoilage Microorganisms

Antimicrobial Activity of Lignin and Lignin-Derived Cellulose and Chitosan Composites against Selected Pathogenic and Spoilage Microorganisms

Antimicrobial Activity of Lignin-Derived Polyurethane Coatings Prepared from Unmodified and Demethylated Lignins

Antibacterial properties of peptides from chia (Salvia hispanica L.) applied to pork meat preservation

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