Antimicrobial activity of
            <i>Mimosa caesalpiniifolia</i>
            Benth and its interaction with antibiotics against
            <i>Staphylococcus aureus</i>
            strains overexpressing efflux pump genes

Silva, S.W.C.; Monção, Nayana Bruna Nery; Araújo, Bruno Quirino; Arcanjo, Daniel Dias Rufino; Ferreira, Josie Haydée Lima; Neto, Joaquim Santos; Citó, Antônia M.G.L.; Júnior, José Pinto de Siqueira; Kaatz, Glenn W.; Barreto, Humberto Medeiros

doi:10.1111/lam.13163

Cited by 6 publications

(5 citation statements)

References 34 publications

(51 reference statements)

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“…A further investigation verified that 4,5,6,7-tetramethoxyflavone, the most methoxylated molecule, exhibited the highest modulating agent performance, reducing the minimal inhibitory concentration of norfloxacin against S. aureus SA1199B by a factor of 16 [47]. However, quercetin, which is a flavonoid analogue of retusin without methoxy groups, has been reported as a TetK and NorA inhibitor [48], suggesting that the mere Attenuation of bacterial drug resistance has been reported for different plant species [49,50]. For Croton species, it has been demonstrated that volatile components from the essential oil of Croton zehntneri potentiate the gentamicin activity against Pseudomonas aeruginosa [40], as well as the norfloxacin activity against S. aureus SA1199B [51].…”

Section: Resultsmentioning

confidence: 95%

Modulatory Effect of Croton heliotropiifolius Kunth Ethanolic Extract on Norfloxacin Resistance in Staphylococcus aureus

Brito,

Alcântara,

Leal

et al. 2023

DDC

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The high frequency of infectious diseases has spurred research into effective tactics to combat microorganisms that are resistant to several drugs. The overproduction of the transmembrane efflux pump protein NorA, which may export hydrophilic fluoroquinolones, is a common mechanism of resistance in S. aureus strains. This work evaluated the antimicrobial activity of the ethanolic extract from the leaves of Croton heliotropiifolius (EECH) against different bacterial and fungal strains, as well as investigating its modulating effect on the resistance to norfloxacin in a Staphylococcus aureus SA1199B overproducing the NorA efflux pump. Microdilution assays were used to assess the EECH’s antibacterial efficacy. The MIC of norfloxacin or ethidium bromide (EtBr) against the SA1199B strain was determined in the presence or absence of the EECH in order to assess the modifying influence on drug resistance. The EECH showed no activity against the Gram-positive and Gram-negative bacterial strains tested. The EECH also showed no antifungal activity against Candida albicans ATCC 10231. On the other hand, the extract reduced the MIC values for norfloxacin against SA1199B at subinhibitory concentrations. In addition, the EECH also reduced the MIC values of EtBr at subinhibitory concentrations, suggesting the occurrence of phytochemicals that inhibit efflux pumps. Molecular docking showed that retusin, a flavonoid found in the extract, could compete with norfloxacin at the orthosteric site of the NorA, indicating that it could be a potential efflux pump inhibitor. However, isolated retusin did not enhance the activity of norfloxacin or EtBr and it did not inhibit the EtBr efflux, showing that it is not a NorA inhibitor. Even though C. heliotropiifolius is a source of phytochemicals that function as adjuvants for norfloxacin, isolated retusin cannot be used in conjunction with norfloxacin to treat infections brought on by S. aureus that overproduces NorA.

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Section: Resultsmentioning

confidence: 95%

Modulatory Effect of Croton heliotropiifolius Kunth Ethanolic Extract on Norfloxacin Resistance in Staphylococcus aureus

Brito,

Alcântara,

Leal

et al. 2023

DDC

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“…Apart from phage endolysins, identifying efflux pump inhibitory molecules, e.g., P-glycoproteins, would be another approach to combat the continuous rise of MDR pathogens [35]. In addition, an array of recent studies has also demonstrated that some natural products such as DCMF from M. caesalpiniifolia and P. amarus ethanoic extracts could function as pump inhibitors and interact with conventional antibiotics fighting against pathogenic resistance [36,37]. These natural or synthetic pumps inhibitors can also be covalently joined together as a hybrid, which indeed showed surprisingly in vitro and in vivo antimicrobial activity [38].…”

Section: Discussionmentioning

confidence: 99%

Design, Overproduction and Purification of the Chimeric Phage Lysin MLTphg Fighting against Staphylococcus aureus

Liu

Deng

et al. 2020

Processes

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With the increasing spread of multidrug-resistant bacterial pathogens, it is of great importance to develop alternatives to conventional antibiotics. Here, we report the generation of a chimeric phage lysin, MLTphg, which was assembled by joining the lysins derived from Meiothermus bacteriophage MMP7 and Thermus bacteriophage TSP4 with a flexible linker via chimeolysin engineering. As a potential antimicrobial agent, MLTphg can be obtained by overproduction in Escherichia coli BL21(DE3) cells and the following Ni-affinity chromatography. Finally, we recovered about 40 ± 1.9 mg of MLTphg from 1 L of the host E. coli BL21(DE3) culture. The purified MLTphg showed peak activity against Staphylococcus aureus ATCC6538 between 35 and 40 °C, and maintained approximately 44.5 ± 2.1% activity at room temperature (25 °C). Moreover, as a produced chimera, it exhibited considerably improved bactericidal activity against Staphylococcus aureus (2.9 ± 0.1 log10 reduction was observed upon 40 nM MLTphg treatment at 37 °C for 30 min) and also a group of antibiotic-resistant bacteria compared to its parental lysins, TSPphg and MMPphg. In the current age of growing antibiotic resistance, our results provide an engineering basis for developing phage lysins as novel antimicrobial agents and shed light on bacteriophage-based strategies to tackle bacterial infections.

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“…The combinations showed synergistic and indifferent effects (Braga et al, 2005). Silva et al (2019) studied the antibacterial activity of the dichloromethane fraction (DCMF) from the stem bark of Mimosa caesalpiniifolia and its effect on the activity of conventional antibiotics against Staphylococcus aureus strains overexpressing specific efflux pump genes. They concluded that such compounds could be used as adjuvants of norfloxacin, ciprofloxacin or tetracycline for treatment of infections caused by S. aureus strains overexpressing efflux pumps (S. W. C. Silva et al, 2019).…”

Section: In Vitro Interaction Assaymentioning

confidence: 99%

“…Additionally, the A. colubrina bark is used as folk medicine for infections and inflammation and there are scientific evidence showing its potential for fighting microorganisms (A. R. N. Lima et al, 2020;R. D. F. Lima et al, 2014;S. W. C. Silva et al, 2019;Weber et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…However, there is a lack of studies that are able to evaluate the antibacterial activity of these extracts against antibiotic-resistant microorganisms, such as methicillin-resistant Staphylococcus aureus (MRSA). Furthermore, there are studies that explore the potential of intact and regenerated A. colubrina bark for the bioprospection of medicines, since natural compounds have been proposed as promising strategies for the treatment of infections (Hiramatsu, 2001;Holetz et al, 2002;Jandú, Silva, Silva, & Correia, 2015;Marques et al, 2018;S. W. C. Silva et al, 2019), especially because it contains three large phenolic compounds (epigallocatechin gallate, tohiperina and gallate) (T.…”

Section: Introductionmentioning

confidence: 99%

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Interaction between bark extract of Anadenanthera colubrina var. cebil (Griseb.) Altschul with antibiotics against methicillin-resistant Staphylococcus aureus (MRSA)

Nascimento

Carvalho

Silva

et al. 2021

RSD

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Anadenanthera colubrina var. cebil (Griseb.) Altschul, a plant often found in areas of the Caatinga in northeastern Brazil, is widely used in unconventional medicine for the treatment of infections and inflammations. Thus, the aim of the present study was to evaluate the antibacterial activity of A. colubrina bark extracts against methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates, to investigate if intact and regenerated bark extracts have the same effect against MRSA and to determine the interaction between these extracts and antibiotics. The antibacterial activity was performed by the determination of the minimum inhibitory concentration (MIC) according to the Clinical and Laboratory Standards Institute (CLSI) and the interaction assay was performed by the checkerboard method. A. colubrina extracts showed bacteriostatic activity (MIC = 8-32 mg/L) against MRSA clinical strains and no difference was found in antibacterial activity between intact and regenerated barks, suggesting that even after regeneration, the barks of this species have the same antibacterial activity. Moreover, the in vitro interaction of A. colubrina extracts with ciprofloxacin or erythromycin was additive (FICI = 0.52). Thus, the bark extracts of Anadenanthera colubrina exhibit antibacterial activity and can be used alone or in combination with antibiotics against MRSA clinical isolates.

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Antimicrobial activity of Mimosa caesalpiniifolia Benth and its interaction with antibiotics against Staphylococcus aureus strains overexpressing efflux pump genes

Cited by 6 publications

References 34 publications

Modulatory Effect of Croton heliotropiifolius Kunth Ethanolic Extract on Norfloxacin Resistance in Staphylococcus aureus

Modulatory Effect of Croton heliotropiifolius Kunth Ethanolic Extract on Norfloxacin Resistance in Staphylococcus aureus

Design, Overproduction and Purification of the Chimeric Phage Lysin MLTphg Fighting against Staphylococcus aureus

Interaction between bark extract of Anadenanthera colubrina var. cebil (Griseb.) Altschul with antibiotics against methicillin-resistant Staphylococcus aureus (MRSA)

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