Antimicrobial activity of<i>Coniothyrium minitans</i>and its macrolide antibiotic macrosphelide A

Tomprefa, Nicola; McQuilken, M. P.; Hill, Robert A.; Whipps, J. M.

doi:10.1111/j.1365-2672.2009.04174.x

Cited by 27 publications

(22 citation statements)

References 28 publications

(49 reference statements)

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“…The results also showed that conidiation of an L-arginine auxotroph could be restored by the NO donor SNP, but not by cGMP. Thus, NO-mediated conidiation has more than one signal pathway, including the cGMP signal pathway and another yet-unknown pathway, and both are essential for conidiation in C. minitans.Coniothyrium minitans is a sclerotial parasite of the notorious plant pathogen Sclerotinia sclerotiorum, and its potential for biological control of Sclerotinia diseases has been well demonstrated in several countries (13,17,21,28,33,34,36,37). Efficient production of conidia will further enhance the potential of C. minitans as a biological control agent.…”

mentioning

confidence: 99%

Cyclic GMP as a Second Messenger in the Nitric Oxide-Mediated Conidiation of the Mycoparasite Coniothyrium minitans

Fù

Xiè

et al. 2010

Appl Environ Microbiol

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Understanding signaling pathways that modulate conidiation of mitosporic fungi is of both practical and theoretical importance. The enzymatic origin of nitric oxide (NO) and its roles in conidiation by the sclerotial parasite Coniothyrium minitans were investigated. The activity of a nitric oxide synthase-like (NOS-like) enzyme was detected in C. minitans as evidenced by the conversion of L-arginine to L-citrulline. Guanylate cyclase (GC) activity was also detected indirectly in C. minitans with the GC-specific inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), which significantly reduced production of cyclic GMP (cGMP). The dynamics of NOS activity were closely mirrored by the cGMP levels during pycnidial development, with the highest levels of both occurring at the pycnidial initiation stage of C. minitans. Furthermore, the NO donor, sodium nitroprusside (SNP), stimulated the accumulation of cGMP almost instantly in mycelium during the hyphal growth stage. When the activity of NOS or GC was inhibited with N-nitro-L-arginine or ODQ, conidial production of C. minitans was suppressed or completely eliminated; however, the suppression of conidiation by ODQ could be reversed by exogenous cGMP. The results also showed that conidiation of an L-arginine auxotroph could be restored by the NO donor SNP, but not by cGMP. Thus, NO-mediated conidiation has more than one signal pathway, including the cGMP signal pathway and another yet-unknown pathway, and both are essential for conidiation in C. minitans.Coniothyrium minitans is a sclerotial parasite of the notorious plant pathogen Sclerotinia sclerotiorum, and its potential for biological control of Sclerotinia diseases has been well demonstrated in several countries (13,17,21,28,33,34,36,37). Efficient production of conidia will further enhance the potential of C. minitans as a biological control agent. Understanding signaling pathways that modulate conidiation of C. minitans will not only facilitate manipulation of the biocontrol agent for commercial use but also advance our understanding of fungal biology.Nitric oxide (NO) is a widespread signaling molecule involved in regulation of a wide range of cellular functions in animals and plants (7). NO synthesis and signaling have been well studied in animals and plants. In mammals, NO plays roles in relaxation of smooth muscle, inhibition of platelet aggregation, neural communication and immune regulation, while in plants NO is involved in disease resistance, abiotic stress, cell death, respiration, senescence, root development, seed germination, and other functions (reviewed in references 6 and 32). NO is also involved in the development of several members of the mycetozoa, such as, Dictyostelium discoideum (10) and Physarum polycephalum (27). The wide variety of effects reflects the basic signaling mechanism that is used by mammals, plants, and virtually all organisms (2). Despite of the extensive research on NO synthesis and signaling processes in animals and plants, our knowledge about NO in fungi is very limi...

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confidence: 99%

Cyclic GMP as a Second Messenger in the Nitric Oxide-Mediated Conidiation of the Mycoparasite Coniothyrium minitans

Fù

Xiè

et al. 2010

Appl Environ Microbiol

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“…This phenomenon is similar to pH-regulation of fumonisin biosynthesis in F. verticillioides, in which pac1 was found to repress fumonisin biosynthesis under neutral ambient pH (Flaherty et al 2003). Tomprefa et al (2009) indicate that AFS or macrosphelide A produced by C. minitans can inhibit mycelial growth of many plant pathogenic fungi including Botrytis spp., Cladosporium cucumerinum, Fusarium oxysporum, Sclerotinia spp., Sclerotium cepivorum, Penicillium nigricans, Phytophthora nicotianae, Pythium ultimum and Rhizoctonia solani. Of these fungi, Sclerotinia spp.…”

Section: Discussionmentioning

confidence: 75%

Molecular cloning, characterization and expression analysis of a pacC homolog in the mycoparasite Coniothyrium minitans

Han

Yang

2010

World J Microbiol Biotechnol

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Coniothyrium minitans is a mycoparasite and a promising biocontrol agent of Sclerotinia sclerotiorum, an omniovorous plant pathogenic fungus. Our previous studies indicate that mycoparasitism and antibiosis of C. minitans are greatly affected by ambient pH, implying that a pH-regulatory system may exist in C. minitans. In this study, we cloned a putative pH-responsive transcription factor gene, cmpacC, from strain Chy-1 of C. minitans. The full-length DNA sequence of cmpacC (GenBank Acc. No. FJ176399) has one open reading frame (ORF) and two introns. cmpacC putatively encodes a polypeptide with 596 amino acid residues (GenBank Acc. No. ACN43303). The sequence of this polypeptide has three conserved zinc finger domains. Meanwhile, a 2,740 bp-long 5 0 flanking sequence of cmpacC was obtained. It contains five copies of PacC binding sequences (5 0 -GCCAAG-3 0 ). Real-time RT-PCR analysis indicated that with the increase of ambient pH from 3 to 8, expression of cmpacC, as well as the chitinase gene (cmch1) and the b-1,3-glucanase gene (cmg1), increased consistently in C. minitans. Cloning of cmpacC from C. minitans suggests that the conserved pHdependent signaling pathway might exist in C. minitans. It provides a clue for further deciphering of the pH-regulatory mechanism responsible for the pH-associated interaction between C. minitans and S. sclerotiorum.

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“…As commented by the EFSA BIOHAZ Panel, available information illustrate the potential of C. minitans to be involved in biocontrol strategy and concern the key role associated to macrosphelide A in the antimicrobial activity of C. minitans (Tomprefa et al, 2009;EFSA BIOHAZ Panel, 2010, 2013. Available information suggests that secondary metabolites/toxins are involved in the mode of action of mycoparasitism.…”

Section: Secondary Metabolites/toxinsmentioning

confidence: 99%

“…This investigation may also be important in relation to efficacy, as inhibition of Bacillus thuringiensis and Bacillus subtilis has been demonstrated, indicating a possible incompatibility with other microbial plant protection products (Tomprefa et al, 2009). Therefore, data on the potential for C. minitans Strain CON/M/91-08 to produce metabolites in relation to these criteria are necessary to assess if the further data requirements and the corresponding risk assessment according to Commission Regulation (EU) No 283/2013, part A, section 7 (standard data requirements and assessment mandatory for chemical plant protections active substances) are triggered.…”

Section: Fate and Behaviour In The Environment Of Any Relevant Metabomentioning

confidence: 99%

Peer review of the pesticide risk assessment of the active substance Coniothyrium minitans Strain CON/M/91‐08

Authority¹

2016

EFS2

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The conclusions of EFSA following the peer review of the initial risk assessments carried out by the competent authorities of the rapporteur Member State, the Netherlands, and co-rapporteur Member State, Estonia, for the pesticide active substance Coniothyrium minitans CON/M/91-08 are reported. The context of the peer review was that required by Commission Implementing Regulation (EU) No 844/2012. The conclusions were reached on the basis of the evaluation of the representative uses of C. minitans CON/M/91-08 as a fungicide by overhead irrigation or incorporation for soil decontamination against Sclerotinia sclerotiorum in winter rape, lettuce, cucumber, beans, oilseed rape, sunflower or applied with the irrigation system in winter rape and lettuce. The reliable end points, appropriate for use in regulatory risk assessment are presented. Missing information identified as being required by the regulatory framework is listed. Concerns are identified.

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Antimicrobial activity ofConiothyrium minitansand its macrolide antibiotic macrosphelide A

Cited by 27 publications

References 28 publications

Cyclic GMP as a Second Messenger in the Nitric Oxide-Mediated Conidiation of the Mycoparasite Coniothyrium minitans

Cyclic GMP as a Second Messenger in the Nitric Oxide-Mediated Conidiation of the Mycoparasite Coniothyrium minitans

Molecular cloning, characterization and expression analysis of a pacC homolog in the mycoparasite Coniothyrium minitans

Peer review of the pesticide risk assessment of the active substance Coniothyrium minitans Strain CON/M/91‐08

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