Antimicrobial activity of globulol isolated from the fruits ofEucalyptus globulusLabill

Abstract

An antimicrobial sesquiterpene was separated by bioassay-guided isolation from the petroleum ether fraction of the ethanol crude extract of Eucalyptus globulus Labill (Myrtaceae) fruits, and was identified as globulol by physicochemical properties and spectroscopic analysis. Mycelial growth method revealed that the median effective inhibitory concentration (IC50) values of globulol on Alternaria solani, Fusarium oxysporum f.sp. niverum, F. graminearum, Rhizoctonia solani and Venturia pirina were 47.1 microg mL(-1), 114.3 microg mL(-1), 53.4 microg mL(-1), 56.9 microg mL(-1), 32.1 microg mL(-1) and 21.8 microg mL(-1), respectively. MTT-colorimetric assay revealed that IC50 values of globulol on Xanthomonas vesicatoria and Bacillus subtilis were 158.0 microg mL(-1) and 737.2 microg mL(-1), respectively. The results indicated that globulol could be a main antimicrobial compound in the ethanol crude extract of E. globulus fruits.

Antimicrobial flavonoids from the twigs ofPopulus nigra × Populus deltoides

“…Over the past decade, there has been elevated interest in the search for antimicrobial agents of plant origin, as well as in isolating and identifying antimicrobial compounds with possible use in integrated plant protection and human disease control (Cowan, 1999;Dixon, 2001;Tan et al, 2008;Zhou et al, 2008). Species and hybrids of Populus (Salicaceae) are of worldwide importance in the production of fibre and energy (Nixon, Stephens, Tyrrel, & Brierley, 2001 poplar cultivar 'Neva', the hybrid of Populus nigra L. Â Populus deltoides Marsh, is one of the most important salicaceous woody plants in subtropical and temperate regions.…”

ANTIBACTERIAL ACTIVITY EVALUATION OF 15 <i>EUCALYPTUS</i> SPECIES ESSENTIAL OILS AGAINST CLINICALLY RELEVANT PATHOGENIC BACTERIA

“…So it is probable that this bactericide effect is due to the presence of some minor compound of the Eucalyptus EO. Previously authors indicated that globulol (an EO minor component) extracted from E. globulus leaf has a strong activity against fungus such as Alternaria solani, Fusarium graminearum, Rhizoctonia solani, Venturia pirina and some bacteria like Xanthomonas vesicatoria and Bacillus subtilis (Manliang et al, 2008). Other authors also relate antimicrobial activity of globulol from E. globulus L. fruits (Tan et al, 2008).…”

“…Previously authors indicated that globulol (an EO minor component) extracted from E. globulus leaf has a strong activity against fungus such as Alternaria solani, Fusarium graminearum, Rhizoctonia solani, Venturia pirina and some bacteria like Xanthomonas vesicatoria and Bacillus subtilis (Manliang et al, 2008). Other authors also relate antimicrobial activity of globulol from E. globulus L. fruits (Tan et al, 2008). Other cause can be the fact that the composition of each essential oil depends on the age of the plant, climate region, season of the year, EOs extraction method, (Cimanga et al, 2002).…”

“…Eucalyptus has been the topic of several studies due to its extensive applications in traditional medicine, mainly in respiratory infections, proving the efficacy of its' products against upper respiratory tract infections (Ben-Arye et al, 2011). Globulol found in Eucalyptus EOs showed a relevant antimicrobial effect (Manliang et al, 2008). It has also been reported that macrocarpals from E. macrocarpa and grandinol from E. perriniana were effective against Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis) (Takahashi et al, 2004).…”

Bioactive Sesquiterpene Obtained from Schinus areira L. (Anacardiaceae) Essential Oil

“…Globulol has been reported as an active sesquiterpenoid with moderate antioxidant ability [19]. This compound has antifungal activity against a variety of fungal species, as well as, bacterial ones [20]. Besides, globulol have the spasmolytic activity [21].…”