Antimicrobial activity of five essential oils against origin strains of the Enterobacteriaceae family

Abstract

An in vitro assay measuring the antimicrobial activity of essential oils of Coridothymus capitatus (Spanish origanum), Satureja montana, Thymus mastichina (Spanish Origanum majorana), Thymus zygis (Spanish variety of Thymus vulgaris) and Origanum vulgare has been carried out against poultry origin strains of Escherichia coli, Salmonella enteritidis and Salmonella essen, and pig origin strains of enterotoxigenic E. coli (ETEC), Salmonella choleraesuis and Salmonella typhimurium. Using the broth microdilution method, all the essential oils showed an MIC > or = 2% (v/v) for the two strains of E. coli. The essential oil that showed the highest antimicrobial activity against the four strains of Salmonella was Origanum vulgare (MIC < or = 1% v/v), followed by Thymus zygis (MIC < or =2% v/v). Thymus mastichina inhibited all the microorganisms at the highest concentration, 4% (v/v), while the rest of the essential oils showed highly variable results. By chemotyping, higher inhibitory capacity was observed in the oils with a higher percentage of phenolic components (carvacrol and thymol) in comparison with oils containing the monoterpenic alcohol linalool. The results of this work confirm the antimicrobial activity of some essential oils, as well as their potential application in the treatment and prevention of poultry and pig diseases caused by salmonella.

Chemical composition, antimicrobial, antioxidant and antitumor activity of Thymus serpyllum L., Thymus algeriensis Boiss. and Reut and Thymus vulgaris L. essential oils

“…As a sole component, thymol was already presented as a good antimicrobial agent in several studies (Penalver et al, 2005;Sokovic et al, 2008Sokovic et al, , 2009. On the other hand, although the lowest thymol content among the three thyme EOs, the oil of T. serpyllum exhibited the strongest activity, implying that, although the thymol is the major oil constituent, obviously it is not the only one responsible for achieved good antimicrobial activity; the involvement of less abundant constituents should also be considered.…”

The in vitro effect of Garden Thyme (Thymus vulgaris L.) extract on Brachyspira hyodysenteriae

“…Their efficiency against a large variety of pathogens and food-borne germs has been proven by recent investigations [11,13,14]. Several of the studies [1,15,16] indicated the sensitivity of Gram-positive bacteria, while others [17][18][19] have shown expressed sensitivity of Gram-negative bacteria.…”

CHEMICAL COMPOSITION, ANTIMICROBIAL, ANTIOXIDANT AND CYTOTOXIC ACTIVITIES OF <i>EUCALYPTUS CHAPMANIANA</i> GROWN IN IRAQ

“…Nevertheless, the main component in other species of Eucalyptus can be a different compound, such as piperitone (E. dives), (E)-methyl cinnamate (E. olida) (Gilles et al, 2010), α-pinene (E. camaldulensis) (Cheng et al, 2009), limonene (E. staigeriana), β-citronellal (E. citriodora) (Maciel et al, 2010), or p-cymene (E. tereticorni) (Toloza et al, 2006). The chemical composition of essential oil is affected by several factors, such as species, geographical location, harvest time, plant part and isolation method (Cimanga et al, 2002;Penalver et al, 2005;Marzoug et al, 2011).…”