Antimicrobial activity of extracts from <i>in vivo</i> and <i>in vitro</i> propagated <i>Lamium album</i> l. plants

Abstract

The antimicrobial activity of 18 different extracts from in vivo and in vitro grown L. album L. plants was evaluated against clinical bacteria and yeasts using the well diffusion method. All the used extracts demonstrated antibacterial activity, whereas only the water extracts from leaves (in vivo) possessed antifungal activity against Candida albicans NBIMCC 72 and Candida glabrata NBIMCC 8673 (14 and 20 mm diameter of inhibition zones and MIC 10 mg/ml, respectively). The methanol and ethanol extracts obtained from the in vitro propagated plants had a broader spectrum of antibacterial activity than those from in vivo plants, while the opposite tendency was observed for the chloroform extracts. All tested flower extracts possessed antimicrobial activity. The chloroform extract from in vivo flowers demonstrated the highest activity against E. faecalis NBIMCC 3915, S. aureus NBIMCC 3703, P. hauseri NBIMCC 1339 and P. aeruginosa NBIMCC 3700 (22 mm, 13 mm, 11 mm, 23 mm zone diameter of inhibition and MIC 0.313 mg/ml, respectively). The water extracts from leaves (both in vivo and in vitro) possessed higher antibacterial activity than extract from flowers. The obtained results showed that both in vivo and in vitro propagated L. album L. could be used as a source of antibacterial substances.

Bioactivity of a Family of Chiral Nonracemic Aminobenzylnaphthols towards Candida albicans

“…The symbol "/" indicate a not significant effect. A significant bioactivity was found for the compounds of the 2nd (4-6) and 3rd series (7)(8)(9), as reported in Figures 1 and 2. Concerning the group of molecules 4-6, their bioactivity was strongly affected by the structure, the concentration and the time.…”

“…Table 2. Two-way ANOVA for the effects of the kind of chemicals and their concentration on the anti-yeast effect after 24 h: comparison of the most promising compounds from the second group (4-6) and the third groups (7)(8)(9) The statistical analysis pointed out a significant effect of the two factors (compound and concentration); moreover, the ANOVA highlighted a significant interaction compound/concentration. The hypothesis decomposition graph shows this type of interaction for compounds 9 and 7; namely the effects of compound 9 were strengthened at 300 µg/mL, whilst molecule 7 underwent to a significant increase of its activity at 450 µg/mL.…”

“…8. The (S,S)-configuration of aminobenzylnaphthol 9, prepared starting from (S)-prolinol, was attributed by analogy to the previously cited combined NMR and computational analysis.…”

“…Some alternatives to the azoles are extracts from plants (like the oils from Iamium album, Cuminum cyminum, Salvadora persica) [8,9], the combination of terpenes and fluconazole [10], bioremediation [11], as well as other agents [12,13].…”

Glandular trichomes, essential oil composition, anti-aspergillus and antioxidative activities of Lamium purpureum L. ethanolic extracts

“…Lamium species ("dead nettles") have been used in folk medicine worldwide, as a remedy in the treatment of several disorders including scrofula, hypertension, paralysis, trauma, fracture, uterine hemorrhage and menorrhagia [5]. These species exhibit multiple biological actions, including antimicrobial [6], antifungal [7], antioxidant [8][9][10][11][12], antiinflammatory and nociceptive [13] as well as anticancer [14,15] activities. Literature data point to the presence of different classes of chemical compounds: including ecdysone and phenylpropanoid glucosides [16], iridoid glucosides [17,18] and oligosaccharides [19].…”