Antimicrobial Activity of Endodontic Sealers on Enterococcus faecalis

Abstract

Enterococcus faecalis has been shown to be highly resistant once established in the root canal system and may play an important role in endodontic failures. The purpose of this study was to evaluate the antimicrobial activity of four root canal sealers on E. faecalis. Seventeen blood-agar plates were inoculated with E. faecalis using the Lawn technique. Five discs were placed on each plate, four with sealers--Sealapex, Roth 811, Kerr EWT, and AH-Plus--and an ampicillin disc as the control. The plates were incubated at 37 degrees C. The zones of inhibition were measured at 24 and 48 h. Analysis using a one-way ANOVA and Tukey test showed a statistically significance difference (p < 0.05) between all four groups of sealers. Roth 811 showed the largest zone of inhibition (1.1 mm), followed by Sealapex (0.8 mm) and Kerr EWT (0.5 mm), whereas AH-Plus had no antimicrobial activity. There was no difference in the zones of inhibition between the 24- and 48-h time periods.

Influence of a polymerizable eugenol derivative on the antibacterial activity and wettability of a resin composite for intracanal post cementation and core build-up restoration

“…However, eugenol below its minimum inhibitory concentration (sub-MIC) has been demonstrated to reduce the virulence properties such as adherence and biofilm formation of the cariogenic bacteria [47,48]. In addition other studies have demonstrated the effect of eugenol on a variety of oral bacteria causing disruption in cell membrane permeability [49] and proton pumps [50] that reduce microbial resistance and biofilm formation on dental materials, which explains the well-known bacteriostatic properties shown in other eugenol containing materials commonly used in dentistry such as zinc oxide eugenol cements and eugenol based root canal sealers [51].…”

Antimicrobial action of calcium hydroxide-based endodontic sealers after setting, against E. faecalis biofilm

“…1,29 Poggio et al 17 used ADT with freshly mixed endodontic sealer (Endomethasone C, Argoseal, Bioseal Normal, Acroseal, AH Plus, Sicura Seal) after 24 and 48 h, and found low antibacterial activity for AH Plus in the periods tested. Wang et al 23 evaluated the proportions of dead and live bacteria inside the dentinal tubules after exposure to root canal sealers by confocal laser scanning microscopy, and reported antimicrobial activity for AH Plus after 1 and 7 days, unlike the results of the present study, which found no antimicrobial activity after 7 days.…”

“…8 Sealapex and Acroseal are sealers containing calcium hydroxide, and their dissociation into calcium and hydroxyl ions alkalinize the environment, leaving it unfavorable to bacterial proliferation. 1 Moreover, calcium ions can react with carbon dioxide and reduce the breathing source of anaerobic bacteria. 9 AH Plus is a sealer with antimicrobial activity against F. nucleatum, P. gingivalis, 10 and E. faecalis.…”

“…However, microorganisms cannot be completely eliminated. 1 Sundqvist et al 2 related that 38% of failed root canal treatments were infected by Enterococcus faecalis. Gram positive cocci facultative anaerobes can overcome the challenges of survival within the root canal system, and resist very harsh environmental conditions, including extreme alkaline pH (11.5), 3 by invading and surviving within dentinal tubules, 4 and enduring prolonged periods of starvation.…”

Antimicrobial Activity of MTA Fillapex, Real Seal SE, Acroseal and Zinc oxide Eugenol Sealers against Enterococcus Faecalis and Candida Albicans

“…Besides that, its results are highly influenced by the diffusibility of the material across the medium. [6,12,13] Therefore, aim of the present study is to evaluate the antimicrobial activity of different root canal sealers against E.faecalis and C.albicans using agar diffusion method.…”