Antimicrobial Activity of Copper Alloys Against Invasive Multidrug-Resistant Nosocomial Pathogens

Eser, Özgen Köseoğlu; Ergin, Alper; Hasçelik, Gülşen

doi:10.1007/s00284-015-0840-8

Cited by 23 publications

(13 citation statements)

References 25 publications

(29 reference statements)

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“…2). A. baumannii clinical isolates have previously been shown to be sensitive to copper-containing metal coupons (50)(51)(52). The data cannot be compared directly due to protocol differences, but similar trends were observed, with full survival on control surfaces, significant reductions on coppercontaining surfaces, and more efficient killing by alloys with a higher copper content.…”

Section: Figmentioning

confidence: 89%

Copper Resistance of the Emerging Pathogen Acinetobacter baumannii

Williams

Neu

Gilbreath

et al. 2016

Appl Environ Microbiol

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Acinetobacter baumannii is an important emerging pathogen that is capable of causing many types of severe infection, especially in immunocompromised hosts. Since A. baumannii can rapidly acquire antibiotic resistance genes, many infections are on the verge of being untreatable, and novel therapies are desperately needed. To investigate the potential utility of copper-based antibacterial strategies against Acinetobacter infections, we characterized copper resistance in a panel of recent clinical A. baumannii isolates. Exposure to increasing concentrations of copper in liquid culture and on solid surfaces resulted in dose-dependent and strain-dependent effects; levels of copper resistance varied broadly across isolates, possibly resulting from identified genotypic variation among strains. Examination of the growth-phase-dependent effect of copper on A. baumannii revealed that resistance to copper increased dramatically in stationary phase. Moreover, A. baumannii biofilms were more resistant to copper than planktonic cells but were still susceptible to copper toxicity. Exposure of bacteria to subinhibitory concentrations of copper allowed them to better adapt to and grow in high concentrations of copper; this copper tolerance response is likely achieved via increased expression of copper resistance mechanisms. Indeed, genomic analysis revealed numerous putative copper resistance proteins that share amino acid homology to known proteins in Escherichia coli and Pseudomonas aeruginosa. Transcriptional analysis revealed significant upregulation of these putative copper resistance genes following brief copper exposure. Future characterization of copper resistance mechanisms may aid in the search for novel antibiotics against Acinetobacter and other highly antibiotic-resistant pathogens. IMPORTANCEAcinetobacter baumannii causes many types of severe nosocomial infections; unfortunately, some isolates have acquired resistance to almost every available antibiotic, and treatment options are incredibly limited. Copper is an essential nutrient but becomes toxic at high concentrations. The inherent antimicrobial properties of copper give it potential for use in novel therapeutics against drug-resistant pathogens. We show that A. baumannii clinical isolates are sensitive to copper in vitro, both in liquid and on solid metal surfaces. Since bacterial resistance to copper is mediated though mechanisms of efflux and detoxification, we identified genes encoding putative copper-related proteins in A. baumannii and showed that expression of some of these genes is regulated by the copper concentration. We propose that the antimicrobial effects of copper may be beneficial in the development of future therapeutics that target multidrug-resistant bacteria.

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Section: Figmentioning

confidence: 89%

Copper Resistance of the Emerging Pathogen Acinetobacter baumannii

Williams

Neu

Gilbreath

et al. 2016

Appl Environ Microbiol

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“…While 63% of participants believed of nonessential, wash their hands. [26] In this case, the necessity for health personnel's implementation of education programs is inevitable. Investigation on hand washing understanding of importance among nurses showed that 40 min face-to-face education significantly effect on increasing the quality and quantity of hand washing.…”

Section: Hands Washing a Prerequisite For Infection Controlmentioning

confidence: 99%

Bacteriological aspects of hand washing: A key for health promotion and infections control

Ataee

Tavana

et al. 2017

Int J Prev Med

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The aim of this review is to show the historical aspects of hands washing for healthy life and explains how can reduce the transmission of community-acquired infectious agents by healthcare workers and patients. This review article is prepared based on available database. The key words used were hands washing, risk assessment, hands hygiene, bacterial flora, contamination, infection, nosocomial, tap water, sanitizer, bacterial resistance, hands bacterial flora, washing methods, antiseptics, healthcare workers, healthcare personnel, from PubMed, ScienceDirect, Embase, Scopus, Web of Sciences, and Google Scholar. Data were descriptively analyzed. The insistence on hand washing has a history of 1400 years. The research results indicate that the bacteria released from the female washed hands in wet and dry condition was lower than from the male's hands with a significance level (3 CFU vs. 8 CFU; confidence interval 95%, P ≤ 0.001). The valuable results of the study indicated that released amount of bacterial flora from wet hands is more than 10 times in compared to dry hands. In addition, established monitoring systems for washing hands before and after patient's manipulation as well as after toilet were dominant indices to prevent the transfer of infectious agents to the patients. Increasing awareness and belief of the healthcare workers have shown an important role by about 30% reduction in the transfection. Hand washing could reduce the episodes of transmission of infectious agents in both community and healthcare settings. However, hand washing is an important key factor to prevent transmission of infectious agents to patients. There is no standard method for measuring compliance. Thus, permanent monitoring of hand washing to reduce the transmission of infections is crucial. Finally, the personnel must believe that hand washing is an inevitable approach to infection control.

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“…On the other hand, copper containing brass showed no major differences in the total bacterial aerobic count loads for door handles and front door handles compared with reference surfaces (20 AE 70 CFU cm À2 and 9 AE 17, P > 0Á05, n = 37) in these real life circumstances. The antimicrobial efficiency of pure copper has been shown to be higher than that of copper alloys such as brass (Noyce et al 2006a;Koseoglu Eser et al 2015;San et al 2015). In the present study, brass and reference material door handles and front door handles had a low occurrence of Gram-negatives, S. aureus and enterococci, respectively (Table 2).…”

Section: Resultsmentioning

confidence: 48%

“…; Koseoglu Eser et al . ). Real life studies in hospitals have shown reduced total microbial counts and a lower occurrence of pathogens in the presence of copper surfaces, such as VRE or MRSA (Casey et al .…”

Section: Introductionmentioning

confidence: 97%

“…The efficiency of copper as an antibacterial material has been noted in laboratory studies on pathogenic microbes such as methicillin-resistant Staphylococcus aureus (MRSA) (Noyce et al 2006a;Mehtar et al 2008;Gould et al 2009), clinical isolates of Escherichia coli, vancomycin resistant Enterococcus spp. (VRE), Candida albicans, Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter baumannii (Mehtar et al 2008;Gould et al 2009;Koseoglu Eser et al 2015). Real life studies in hospitals have shown reduced total microbial counts and a lower occurrence of pathogens in the presence of copper surfaces, such as VRE or MRSA (Casey et al 2010;Mikolay et al 2010;Karpanen et al 2012;Schmidt et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Copper as an antibacterial material in different facilities

Inkinen

Mäkinen

Keinänen‐Toivola

et al. 2016

Lett Appl Microbiol

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The efficiency of copper as an antimicrobial material has been noted in laboratory studies and in the hospital environment. The present study further shows that copper exerted an antibacterial effect in different facilities, i.e. in a hospital, a kindergarten, an office building and in a retirement home for the elderly. The study suggests that copper has potential use as an antibacterial material and therefore might serve as a means to lower the incidence of transmission of infectious agents from inanimate surfaces in different facilities, with everyday functions.

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Antimicrobial Activity of Copper Alloys Against Invasive Multidrug-Resistant Nosocomial Pathogens

Cited by 23 publications

References 25 publications

Copper Resistance of the Emerging Pathogen Acinetobacter baumannii

Copper Resistance of the Emerging Pathogen Acinetobacter baumannii

Bacteriological aspects of hand washing: A key for health promotion and infections control

Copper as an antibacterial material in different facilities

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