Antimicrobial Activity of Apis mellifera Bee Venom Collected in Northern Peru

Pérez-Delgado, Orlando; Espinoza-Culupú, Abraham; López-López, Elmer

doi:10.3390/antibiotics12040779

Cited by 3 publications

(3 citation statements)

References 74 publications

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“…Pérez-Delgado et al [60] evaluated the antibacterial activity of Apis mellifera venom against E. coli, P. aeruginosa, and S. aureus. The outcome of their study showed the minimum inhibitory concentration (MIC) of 6.88 µg/mL against E. coli with no satisfactory results against P. aeruginosa, and S. aureus.…”

Section: Molecular Dynamics (Md) Simulationmentioning

confidence: 99%

In Silico Analysis of Honey Bee Peptides as Potential Inhibitors of Capripoxvirus DNA-Directed RNA Polymerase

Mustafa

Mahrosh

Salman

et al. 2023

Animals

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The genus Capripoxvirus belongs to the Poxviridae family. The sheeppox, goatpox, and lumpy skin disease viruses are three species of this genus with 96% identity in their genomes. These are financially devastating viral infections among cattle, which cause a reduction in animal products and lead to a loss in livestock industries. In the current study, the phylogenetic analysis was carried out to reveal the evolutionary relationships of Capripoxvirus species (i.e., sheeppox virus (SPPV), goatpox virus (GTPV), and lumpy skin disease virus (LSDV)) with other viruses from the Poxviridae family with >96% query coverage to find the similarity index among all members. The three viruses (i.e., SPPV, GTPV, and LSDV) joined the clade of Capripoxvirus of the Poxviridae family in the phylogenetic tree and exhibited close evolutionary relationships. The multiple sequence alignment using ClustalOmega revealed significant variations in the protein sequences of the DNA-dependent RNA polymerase of SPPV, GTPV, and LSDV. The three-dimensional structures of five selected bee peptides and DNA-directed RNA polymerase of SPPV, GTPV, and LSDV were predicted using trRosetta and I-TASSER and used for molecular docking and simulation studies. The protein–protein docking was carried out using HADDOCK server to explore the antiviral activity of peptides as honey bee proteins against SPPV, GTPV, and LSDV. In total, five peptides were docked to DNA-directed RNA polymerase of these viruses. The peptides mellitin and secapin-1 displayed the lowest binding scores (−106.9 +/− 7.2 kcal/mol and −101.4 +/− 11.3 kcal/mol, respectively) and the best patterns with stable complexes. The molecular dynamics simulation indicated that the complex of protein DNA-dependent RNA polymerase and the peptide melittin stayed firmly connected and the peptide binding to the receptor protein was stable. The findings of this study provide the evidence of bee peptides as potent antimicrobial agents against sheeppox, goatpox, and lumpy skin disease viruses with no complexity.

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Section: Molecular Dynamics (Md) Simulationmentioning

confidence: 99%

In Silico Analysis of Honey Bee Peptides as Potential Inhibitors of Capripoxvirus DNA-Directed RNA Polymerase

Mustafa

Mahrosh

Salman

et al. 2023

Animals

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“…A series of recent studies indicated that BV has a wide range of microbial activity against bacteria, fungi, and viruses ( El-Didamony et al, 2022b ; Pérez-Delgado et al, 2023 ), as well as treating different types of cancer cells ( Taher et al, 2017 ; El-Didamony et al, 2022a ). Overall, BV and its selective components are considered promising agents for disease management.…”

Section: Introductionmentioning

confidence: 99%

Melittin alcalase-hydrolysate: a novel chemically characterized multifunctional bioagent; antibacterial, anti-biofilm and anticancer

El-Didamony,

Kalaba,

Sharaf

et al. 2024

Front. Microbiol.

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The prevalent life-threatening microbial and cancer diseases and lack of effective pharmaceutical therapies created the need for new molecules with antimicrobial and anticancer potential. Bee venom (BV) was collected from honeybee workers, and melittin (NM) was extracted from BV and analyzed by urea-polyacrylamide gel electrophoresis (urea-PAGE). The isolated melittin was hydrolyzed with alcalase into new bioactive peptides and evaluated for their antimicrobial and anticancer activity. Gel filtration chromatography fractionated melittin hydrolysate (HM) into three significant fractions (F1, F2, and F3), that were characterized by electrospray ionization mass spectrometry (ESI-MS) and evaluated for their antimicrobial, anti-biofilm, antitumor, and anti-migration activities. All the tested peptides showed antimicrobial and anti-biofilm activities against Gram-positive and Gram-negative bacteria. Melittin and its fractions significantly inhibited the proliferation of two types of cancer cells (Huh-7 and HCT 116). Yet, melittin and its fractions did not affect the viability of normal human lung Wi-38 cells. The IC50 and selectivity index data evidenced the superiority of melittin peptide fractions over intact melittin. Melittin enzymatic hydrolysate is a promising novel product with high potential as an antibacterial and anticancer agent.

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“…The lack of potential benefits from the combination between frankincense oil and the tested antibiotics is discouraging; however, the in vivo results allow us to suggest that the oil, per se, may act as a modulator of inflammatory responses in infectious diseases. Another interesting study by Pérez-Delgado [ 18 ] explored the in vitro antibacterial activity of Apis mellifera, venom collected from bees of Lambayeque, northern Peru, against E. coli , Pseudomonas aeruginosa , and Staphylococcus aureus reference strains. Three peptides of low molecular weights (5 kDa, 6 kDa, and 7 kDa) were separated from the crude bee venom.…”

mentioning

confidence: 99%

Antimicrobial and Anti-Infective Activity of Natural Products—Gaining Knowledge from Novel Studies

Fernandes

Figueiredo²,

Monteiro

et al. 2023

Antibiotics

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Despite advances in the development of antimicrobial drugs in the last centuries, antimicrobial resistance has consistently raised in the last decades, compromising their effectiveness. Novel antimicrobial compounds, especially from natural sources, including plants, microorganisms, and animals, have since become a growing area of research. In this context, studies covering the investigation of their ability to combat resistant microorganisms, either by neutralization or inactivation of pathogen resistance mechanisms and virulence properties, have gained attention. Herein, a collection of 19 manuscripts focused on the antimicrobial and anti-infective activity of natural products, including their mechanisms of action, in silico evidence of antimicrobial activity, synergistic associations with antibiotics, and other aspects, will be discussed.

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Antimicrobial Activity of Apis mellifera Bee Venom Collected in Northern Peru

Cited by 3 publications

References 74 publications

In Silico Analysis of Honey Bee Peptides as Potential Inhibitors of Capripoxvirus DNA-Directed RNA Polymerase

In Silico Analysis of Honey Bee Peptides as Potential Inhibitors of Capripoxvirus DNA-Directed RNA Polymerase

Melittin alcalase-hydrolysate: a novel chemically characterized multifunctional bioagent; antibacterial, anti-biofilm and anticancer

Antimicrobial and Anti-Infective Activity of Natural Products—Gaining Knowledge from Novel Studies

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