Antimicrobial activity of amalgams, alloys and their elements and phases

Morrier, Jean-Jacques; Suchett-Kaye, G; Nguyen, Daniel H.; Rocca, Jean‐Paul; Blanc-Benon, J; Barsotti, Odile

doi:10.1016/s0109-5641(98)00022-0

Cited by 46 publications

(38 citation statements)

References 38 publications

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“…Some materials can release ions with cariostatic properties that may reduce caries development through inhibition of bacterial growth. Amalgam, for instance, releases cariostatic agents, such as Ag, Cu, and Zn ions (Nunez et al 1976;Orstavik 1985;Morrier et al 1998). Other materials, like glass ionomer cement, release fluoride, which enhances remineralization.…”

Section: Introductionmentioning

confidence: 99%

Restoration Materials and Secondary Caries Using an In Vitro Biofilm Model

et al. 2014

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This in vitro study investigated whether restoration materials and adhesives influence secondary caries formation in gaps using a short-term in vitro biofilm model. Sixty enamel-dentin blocks were restored with 6 different restoration materials with or without adhesives (n = 10 per group) with a gap: 1) Clearfil AP-X composite, 2) Clearfil AP-X composite + SE Bond, 3) Clearfil AP-X composite + ProtectBond, 4) Filtek Silorane composite, 5) Filtek Silorane composite + Silorane System adhesive, or 6) Tytin amalgam. Specimens were subjected to an intermittent 1% sucrose biofilm model for 20 days to create artificial caries lesions. Lesion progression in the enamel-dentin next to the different materials was measured in lesion depth (LD) and mineral loss (ML) using transversal wavelength independent microradiography (T-WIM). A regression analysis was used to compare the LD and ML of the different restoration materials at 4 measurement locations: 1 location at the surface of the enamel, 1 location at the wall of the enamel, and 2 locations at the wall of the dentin. A statistically significant effect of AP-X composite with Protect Bond was found for LD and ML at the WallDentin1 location, leading to less advanced wall lesions. An additional finding was that gap size was also statistically significant at the 2 wall locations in dentin, leading to increasing lesion progression with wider gaps. In conclusion, adhesives can influence wall lesion development in gaps. Protect Bond showed significantly less caries progression compared to bare restoration materials or other adhesives in this short-term in vitro biofilm model.

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Section: Introductionmentioning

confidence: 99%

Restoration Materials and Secondary Caries Using an In Vitro Biofilm Model

et al. 2014

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“…In hospitals, copper alloys, used in doorknobs and other surfaces, exerted an in vitro antimicrobial effect against Escherichia coli O157, methicillin-resistant Staphylococcus aureus (MRSA) and Clostridium difficile while equivalent stainless steel surfaces did not [5][6][7][8]. Copper and zinc amalgams have proven useful in dental materials [6] while their salts have been incorporated into mouthwashes for the treatment of gingivitis [9].…”

Section: Introductionmentioning

confidence: 99%

Preparation and rapid analysis of antibacterial silver, copper and zinc doped sol–gel surfaces

Jaiswal

McHale²,

Duffy

2012

Colloids and Surfaces B: Biointerfaces

137

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“…mutans dwells, is an environment of highly fluctuating levels of nutrients and essential elements. Metal ions such as those of copper can come from consumed foods and from material, such as amalgam, used in dental restorations (Morrier et al, 1998 ;Wataha & Lockwood, 1998). To date, there have been no reports on the mechanism by which Strep.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a copper-transport operon, copYAZ, from Streptococcus mutans The GenBank accession number for the cop operon sequence reported in this paper is AF296446.

Vats

Lee

2001

Microbiology

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A copper-transport (copYAZ) operon was cloned from the oral bacterium Streptococcus mutans JH1005. DNA sequencing showed that the operon contained three genes (copY, copA and copZ), which were flanked by a single promoter and a factor-independent terminator. copY encoded a small protein of 147 aa with a heavy-metal-binding motif (CXCX 4 CXC) at the C-terminus. CopY shared extensive homology with other bacterial negative transcriptional regulators. copA encoded a 742 aa protein that shared extensive homology with P-type ATPases. copZ encoded a 67 aa protein that also contained a heavymetal-binding motif (CXXC) at the N-terminus. Northern blotting showed that a 32 kb transcript was produced by Cu 2M -induced Strep. mutans cells, suggesting that the genes were synthesized as a polycistronic message. The transcriptional start site of the cop operon was mapped and shown to lie within the inverted repeats of the promoter-operator region. Strep. mutans wild-type cells were resistant to 800 µM Cu 2M , whereas cells of a cop knock-out mutant were killed by 200 µM Cu 2M . Complementation of the cop knock-out mutant with the cop operon restored Cu 2M resistance to wild-type level. The wild-type and the mutant did not show any differences in susceptibility to other heavy metals, suggesting that the operon was specific for copper. By using a chloramphenicol acetyltransferase reporter gene fusion, the cop operon was shown to be negatively regulated by CopY and could be derepressed by Cu 2M .

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Antimicrobial activity of amalgams, alloys and their elements and phases

Cited by 46 publications

References 38 publications

Restoration Materials and Secondary Caries Using an In Vitro Biofilm Model

Restoration Materials and Secondary Caries Using an In Vitro Biofilm Model

Preparation and rapid analysis of antibacterial silver, copper and zinc doped sol–gel surfaces

Characterization of a copper-transport operon, copYAZ, from Streptococcus mutans The GenBank accession number for the cop operon sequence reported in this paper is AF296446.

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