Antimicrobial activity in medicinal plants of the Scrophulariaceae and Acanthaceae

Meurer-Grimes, Barbara; McBeth, Dani L.; Hallihan, Betsey; Delph, Sharmaine

doi:10.1076/phbi.34.4.243.13220

Cited by 50 publications

(29 citation statements)

References 9 publications

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“…The herbs are constantly being screened for their biological and pharmacological activities such as anti-diabetic, anti-oxidant, anti-microbial, laxative, and anti-cancer activities [2][3][4][5][6][7]. The herbs are having numerous bio active components which are identified ( at less than 1 ng ) by using GC or LC-MS. Spectroscopic (UV-Vis, FTIR) methods together or separate can be used because of its simplecity, cost-effective and rapid tests for detecting phytocomponents.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Analysis of Hybanthus enneaspermus using UV, FTIR and GC- MS

Anand¹

2012

iosrphr

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The present study was carried out to characterize the bioactive constituents present in ethanolic extracts of Hybanthus enneaspermus using UV, FTIR and GC-MS. The crude extracts were scanned in the wavelength ranging from 300-1100 nm by using Perkin Elmer Spectrophotometer and the characteristic peaks were detected. For GC-MS analysis, 20 g sample is extracted with 50 ml ethanol, filtered in ash less filter paper with 4 g sodium sulphate and the extract is concentrated to 1 ml by bubbling nitrogen into the solution. The compound detection employed the NIST Ver. 2.0 -Year 2005 library. The biological activities are based on Dr. Duke's Phytochemical and Ethnobotanical Databases by Dr. Jim Duke of the Agricultural Research Service/USDA. The UV profile showed different peaks ranging from 300-1100 nm with different absorption respectively. The FTIR spectrum confirmed the presence of phenols, alcohols, alkanes, alkyl halides, carboxylic acids, aromatics, nitro compounds and amines in ethanolic extract. The results of the GC-MS analysis provide different peaks determining the presence of phytochemical compounds with different therapeutic activities. The major phytoconstituents were (5E,13E)-5,13-Docosadienoic acid (20.90%) and Cedran-diol, 8S, 14-(13.02) which are possessing many biological activities. hence this study creates a platform to screen many bioactive components to treat many diseases.

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Section: Introductionmentioning

confidence: 99%

Phytochemical Analysis of Hybanthus enneaspermus using UV, FTIR and GC- MS

Anand¹

2012

iosrphr

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“…Also, natural products from microorganisms have been the primary source of antibiotics, but with the increasing acceptance of herbal medicine as an alternative form of health care, the screening of medicinal plants for active compounds has become very important because these may serve as promising sources of novel antibiotic prototypes [21][22][23]. It has been shown that in vitro screening methods could provide the needed preliminary observations necessary to select crude plant extracts with potentially useful properties for further chemical and pharmacological investigations [24].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Polyphenolic, Nutritive and Biological Activities of the Acetone, Methanol and Water Extracts of Amaranthus asper~!2009-06-16~!2010-02-02~!2010-04-14~!

Jimoh¹,

Adedapo²,

Aliero³

et al. 2010

TOALTMEDJ

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Abstract:The nutritional, phytochemical, antioxidant and antibacterial activities of the acetone, methanol and water extracts of the leaves of Amaranthus asper were investigated using standard analytical methods in order to assess the numerous potential of the plant leaves. The proximate analysis showed that the leaves of this plant contained moisture, ash, crude protein, crude lipid, crude fibre, energy and carbohydrate. Elemental analysis in mg/100 g (DW) indicated that the leaves contained sodium (0.0373), potassium (2.872), calcium (2.504), Magnesium (0.569), Iron (419), Zinc (26), phosphorus (0.177), Copper (18), Manganese (91), and Nitrogen (1.78). The chemical composition in mg/100 g (DW) for alkaloid, saponins, and phytate were 0.3, 7.0 and 9.25 respectively. Comparing the nutrient and chemical constituents with recommended dietary allowance (RDA) values, the results reveal that the leaves contain an appreciable amount of nutrients, minerals, and phytochemicals and low levels of toxicants. At 1mg/ml, the acetone extract caused 98.2% ABTS radical scavenging inhibition while the methanol, water and BHT caused inhibition at 95.4, 97.1 and 99.3% respectively. The result for DPPH scavenging activity was close to that of ABTS. The plant was also rich in the different polyphenols analyzed in this study. The ferrous reducing antioxidant power (FRAP) values for acetone extract (1084.9) is much higher than that of methanol (143.5), water (42.4), BHT (63.5), and catechin (972.02) but lower than those of ascorbic acid (1632.1) and quercetin (3107.3). The methanol extract has antibacterial activity against all the Gram-positive organisms used in this study. This study has to some extent validated the need for the use of the leaves of Amaranthus asper for nutritional and medicinal purposes.

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“…Such plants have continued to be used for primary healthcare not only in poor developing countries, but also in countries where conventional medicine is predominant in the national healthcare system (Lanfranco, 1999). The screening of plant extracts for antimicrobial activity has shown that higher plants represent a potential source of novel antibiotic prototypes (Maurer-Grimes et al, 1996;Rabe & van Staden, 1997;Afolayan, 2003).…”

Section: Introductionmentioning

confidence: 99%