Antimicrobial activity evaluation for gentamicin loaded PLA microspheres

Sabee, M. M. S. Mohd; Awang, Mohd Syafiq; Bustami, Yazmin; Hamid, Zuratul Ain Abdul

doi:10.1016/j.matpr.2019.06.092

Cited by 2 publications

(2 citation statements)

References 13 publications

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“…Many researchers have investigated this material, as it exhibits great potential for use in bone tissue engineering [ 54 ]. Different forms of this biocompatible polymer are being used, such as microspheres [ 55 , 56 , 57 ], membranes [ 58 , 59 , 60 ] or scaffolds [ 54 , 61 , 62 , 63 ]. One of the methods used to fabricate porous polymer scaffolds is the gel-casting method [ 37 ], which is a simple and effective way to obtain samples with random porosity with a pore size in a range from100 to 1500 µm.…”

Section: Discussionmentioning

confidence: 99%

Characterization and In Vitro Evaluation of Porous Polymer-Blended Scaffolds Functionalized with Tricalcium Phosphate

Pudełko-Prażuch,

Balasubramanian,

Ganesan

et al. 2024

JFB

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Bone tissue is one of the most transplanted tissues. The ageing population and bone diseases are the main causes of the growing need for novel treatments offered by bone tissue engineering. Three-dimensional (3D) scaffolds, as artificial structures that fulfil certain characteristics, can be used as a temporary matrix for bone regeneration. In this study, we aimed to fabricate 3D porous polymer scaffolds functionalized with tricalcium phosphate (TCP) particles for applications in bone tissue regeneration. Different combinations of poly(lactic acid) (PLA), poly(ethylene glycol) (PEG with molecular weight of 600 or 2000 Da) and poly(ε-caprolactone) (PCL) with TCP were blended by a gel-casting method combined with rapid heating. Porous composite scaffolds with pore sizes from 100 to 1500 µm were obtained. ATR-FTIR, DSC, and wettability tests were performed to study scaffold composition, thermal properties, and hydrophilicity, respectively. The samples were observed with the use of optical and scanning electron microscopes. The addition of PCL to PLA increased the hydrophobicity of the composite scaffolds and reduced their susceptibility to degradation, whereas the addition of PEG increased the hydrophilicity and degradation rates but concomitantly resulted in enhanced creation of rounded mineral deposits. The scaffolds were not cytotoxic according to an indirect test in L929 fibroblasts, and they supported adhesion and growth of MG-63 cells when cultured in direct contact.

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Section: Discussionmentioning

confidence: 99%

Characterization and In Vitro Evaluation of Porous Polymer-Blended Scaffolds Functionalized with Tricalcium Phosphate

Pudełko-Prażuch,

Balasubramanian,

Ganesan

et al. 2024

JFB

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“…The used micro-stent coating material PLLA is a well-known and established coating material for cardiovascular devices and controlled drug release systems [11] including direct mixing or adsorption of antibiotics or drug encapsulation in microspheres or coreshell porous nanofibers to avoid burst-release. [12][13][14] Therefore, this material is an ideal candidate for the validation of our in vivo model for testing antimicrobial materials in the bloodstream for cardiovascular devices. The micro-stent can be coated with other polymeric material or chemically functionalized, e.g., with antibiotics, antimicrobial peptides, or antifouling coatings.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a Murine Model for Testing Antimicrobial Implant Materials in the Blood Circulation System

et al. 2021

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Medical device-related infections are becoming a steadily increasing challenge for the health care system regarding the difficulties in the clinical treatment. In particular, cardiovascular implant infections, catheter-related infections, as well as infective endocarditis are associated with high morbidity and mortality risks for the patients. Antimicrobial materials may help to prevent medical device-associated infections and supplement the currently available therapies. In this study, we present an easy-to-handle and simplified in vivo model to test antimicrobial materials in the bloodstream of mice. The model system is composed of the implantation of a bacteria-laden micro-stent scaffold into the murine tail vein. Our model enables the simulation of catheter-related infections as well as the development of infective endocarditis specific pathologies in combination with material testing. Furthermore, this in vivo model can cover two phases of the biofilm formation, including both the local tissue response to the bacterial biofilm and the systemic inflammatory response against circulating bacteria in the bloodstream that detached from a mature biofilm.

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Antimicrobial activity evaluation for gentamicin loaded PLA microspheres

Cited by 2 publications

References 13 publications

Characterization and In Vitro Evaluation of Porous Polymer-Blended Scaffolds Functionalized with Tricalcium Phosphate

Characterization and In Vitro Evaluation of Porous Polymer-Blended Scaffolds Functionalized with Tricalcium Phosphate

Evaluation of a Murine Model for Testing Antimicrobial Implant Materials in the Blood Circulation System

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