Antimicrobial Activity and Physicochemical Properties of Antibiotic Pastes Used In Regenerative Endodontics

Zancan, Rafaela Fernandes; Cavenago, Bruno Cavalini; Oda, Denise Ferracioli; Bramante, Clóvis Monteiro; Andrade, Flaviana Bombarda de; Duarte, Marco Antônio Húngaro

doi:10.1590/0103-6440201902613

Cited by 18 publications

(34 citation statements)

References 22 publications

(25 reference statements)

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“…do Couto et al stated that the disinfection of the immature teeth in REP can be judiciously achieved with the use of triple antibiotic paste (TAP) 6 . Although the current clinical recommendations by the American Association of Endodontists (AAE) for REP suggests the use of TAP or calcium hydroxide, when comparatively analysing, TAP and double antibiotic paste showed increased antimicrobial activity compared to calcium hydroxide 7 .…”

Section: Introductionmentioning

confidence: 97%

Prospective cohort study of regenerative potential of non vital immature permanent maxillary central incisors using platelet rich fibrin scaffold

Kritika

Sujatha

Srinivasan

et al. 2021

Sci Rep

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Regenerative endodontic procedures have gained momentum as a treatment modality of young immature permanent teeth. Literature reports reveal that platelet-rich fibrin (PRF) stimulates growth factors and induces regeneration. This study was undertaken to assess the regenerative potential of non-vital immature permanent maxillary central incisors using PRF with a follow-up for 2 yrs. 19 patients in the age group of 9–25 yrs with immature, non-vital permanent maxillary central incisors (n = 23) with/without signs and/or symptoms of periapical pathosis and open apex were included in this study. In the first appointment, access opening, canal disinfection and triple antibiotic paste placement were done. In the subsequent visit, PRF was prepared and placed inside the canal. Access was sealed with Mineral trioxide aggregate plug and composite. The patient was reviewed up to 24 mths. The mean difference was statistically analyzed using Friedman test followed by Dunn post hoc test and adjusted by Bonferroni correction (p < 0.05). As per AAE guidelines, the primary and secondary goals were achieved. A significant (p < 0.001) gradual increase in the root length, thickness of dentinal walls and decrease in apical diameter were observed. Within the limitations of this study, PRF placement was clinically and radiographically effective in inducing regeneration of non-vital immature permanent teeth.

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Section: Introductionmentioning

confidence: 97%

Prospective cohort study of regenerative potential of non vital immature permanent maxillary central incisors using platelet rich fibrin scaffold

Kritika

Sujatha

Srinivasan

et al. 2021

Sci Rep

View full text Add to dashboard Cite

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“…Another recent study has demonstrated through confocal microscopy that Ca(OH) 2 had a low level of antimicrobial action against E . faecalis biofilm (Zancan et al, 2019b). Both DAP and TAP were effective against this biofilm after seven days of incubation on dentine discs (Zancan et al, 2019b).…”

Section: Discussionmentioning

confidence: 99%

“…faecalis biofilm (Zancan et al, 2019b). Both DAP and TAP were effective against this biofilm after seven days of incubation on dentine discs (Zancan et al, 2019b).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial and immunomodulatory in vitro profile of double antibiotic paste

et al. 2021

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Aim To evaluate the antimicrobial and immunomodulatory activity of double antibiotic paste (DAP) in an in vitro infection model. Methodology The minimum inhibitory and bactericidal concentrations (MIC and MBC) and the antibiofilm activities (TTC assay) of DAP and its components (ciprofloxacin and metronidazole) were evaluated against Staphylococcus aureus and Enterococcus faecalis compared with triple antibiotic paste (TAP). The cellular viability of RAW 264.7 macrophages (24 and 72 h) and L929 fibroblasts (48 and 72 h) was evaluated by MTT. Furthermore, the production of TNF‐α, IL‐12, IL‐6, IL‐1α, IL‐10 and NO (on RAW 264.7), besides IL‐6, TGF‐β and NO (on L929), stimulated with DAP in baseline and associated with heat‐killed microbial‐antigen conditions was measured by ELISA and Griess reaction. Data were analysed using the one‐way ANOVA test with Bonferroni's corrections. Results The MBC of pharmacopoeia DAP was similar to TAP for E. faecalis (0.25 μg. mL−1) and lower for S. aureus (DAP 1 μg. mL−1 and TAP 2 μg. mL−1; p < .001). Ciprofloxacin was the most effective antibiofilm drug from the pastes (35% of reduction for E. faecalis and S. aureus; p < .0001), and both pastes had a similar antibiofilm eradication against both biofilm species (29% and 35% for S. aureus and 76% and 85% for E. faecalis; p < .0001). DAP was cytotoxic against the tested cells. DAP significantly upregulated IL‐1α (p < .001), IL‐6 (p < .0001), TNF‐α (p < .01) and IL‐12 (p < .05; in the absence of antigens) and significantly reduced IL‐6 (p < .0001; in the presence of HK‐S. aureus) and IL‐10 (p < .05; in the presence of both antigens) on macrophages. Furthermore, DAP upregulated IL‐6 (p < .001) and NO (p < .05; in the absence of antigens), IL‐6 (p < .001; in the presence of HK‐S. aureus) and reduced NO (p < .001; in the presence of HK‐S. aureus). Conclusions Double antibiotic paste and TAP had similar antimicrobial activity against S. aureus and E. faecalis. DAP upregulated pro‐inflammatory cytokines mainly in the absence of antigens and had pro‐ and anti‐inflammatory activity in RAW 264.7 macrophages and L929 fibroblasts in the presence of antigens involved in pulp infections.

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“…A variety of decontamination protocols for RET have been reported. Most use sodium hypochlorite as irrigant solution and triple (metronidazole, ciprofloxacin, and minocycline) (TAP) (7,9) or double (metronidazole/ ciprofloxacin) (DAP) (10,11) antibiotic paste as intracanal medicament. Both substances have antimicrobial activity against endodontic pathogens (10)(11)(12)(13), which allows repair of apical tissues, cellular induction and reestablishment of root formation (10).…”

Section: Introductionmentioning

confidence: 99%

12-Year Follow-Up of Regenerative Endodontic Treatment of Immature Permanent Upper Incisors with Acute Abscess

et al. 2020

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This case report discusses the endodontic treatment of a 7-year-old girl who suffered trauma (intrusion) to the immature upper central incisors secondary to a fall from a bicycle. Thirty days after the accident the patient was brought by her mother for clinical and radiographic assessment with a chief complaint of swelling and tenderness to percussion and palpation. Acute apical abscess associated with immature teeth were diagnosed. A decision was made to perform regenerative endodontic treatment. Access cavities were made and the root canals were disinfected by irrigation with 2.5% sodium hypochlorite. Final irrigation was performed with 17% EDTA. Due to pain and presence of secretions, 2% chlorhexidine gel was applied as an intracanal medicament. Seven days later, at the second visit, the root canals were once again disinfected and the canals of the right and left permanent upper central incisors were filled with double antibiotic paste (metronidazole/ciprofloxacin) and calcium hydroxide paste, respectively. Zinc oxide was mixed in both pastes. At the third visit, after 21 more days, the pastes were removed and the periapical areas were stimulated with a #80 K-file to encourage clot formation within the pulp cavities. A mineral trioxide aggregate (MTA) paste cervical plug was placed and the teeth were restored with glass ionomer cement. Radiographs and CBCT scans demonstrated complete root formations. The patient has been followed for 12 years, with evidence of clinical success throughout.

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Antimicrobial Activity and Physicochemical Properties of Antibiotic Pastes Used In Regenerative Endodontics

Cited by 18 publications

References 22 publications

Prospective cohort study of regenerative potential of non vital immature permanent maxillary central incisors using platelet rich fibrin scaffold

Prospective cohort study of regenerative potential of non vital immature permanent maxillary central incisors using platelet rich fibrin scaffold

Antimicrobial and immunomodulatory in vitro profile of double antibiotic paste

12-Year Follow-Up of Regenerative Endodontic Treatment of Immature Permanent Upper Incisors with Acute Abscess

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