Antimicrobial activity and mechanism of Larch bark procyanidins against &amp;lt;italic&amp;gt;Staphylococcus aureus&amp;lt;/italic&amp;gt;

Li, Xinchao; He, Congfen; Song, Liya; Li, Ting; Cui, Shumei; Zhang, Liping; Jia, Yan

doi:10.1093/abbs/gmx112

Cited by 60 publications

(47 citation statements)

References 15 publications

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“…Glucose decomposition of microorganism occurs through three pathways including the hexose monophophate pathway (HMP), the Embden-Meyerhof-Parnas pathway (EMP), and the tricarboxylic acid cycle (TCA) [46]. The three corresponding typical respiratory inhibitors are sodium phosphate tribasic dodecahydrate, iodoacetic acid, and malonic acid.…”

Section: Resultsmentioning

confidence: 99%

Antibacterial Activity and Mode of Action of Dihydromyricetin from Ampelopsis grossedentata Leaves against Food-Borne Bacteria

et al. 2019

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Dihydromyricetin (DMY) has recently attracted increased interest due to its considerable health-promoting activities but there are few reports on its antibacterial activity and mechanism. In this paper, the activity and mechanisms of DMY from Ampelopsis grossedentata leaves against food-borne bacteria are investigated. Moreover, the effects of pH, thermal-processing, and metal ions on the antibacterial activity of DMY are also evaluated. The results show that DMY exhibits ideal antibacterial activity on five types of food-borne bacteria (Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Salmonella paratyphi, and Pseudomonas aeruginosa). The activities of DMY against bacteria are extremely sensitive to pH, thermal-processing, and metal ions. The morphology of the tested bacteria is changed and damaged more seriously with the exposure time of DMY. Furthermore, the results of the oxidative respiratory metabolism assay and the integrity of the cell membrane and wall tests revealed that the death of bacteria caused by DMY might be due to lysis of the cell wall, leakage of intracellular ingredients, and inhibition of the tricarboxylic acid cycle (TCA) pathway.

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Section: Resultsmentioning

confidence: 99%

Antibacterial Activity and Mode of Action of Dihydromyricetin from Ampelopsis grossedentata Leaves against Food-Borne Bacteria

et al. 2019

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“…The antibacterial activity of polyphenols is realized at the level of whole bacteria through the damage (modification) of the membrane structure [5][6][7] and via inhibition of energy metabolism 8 , production or secretion of toxins [9][10][11][12] , as well as by prevention of biofilm formation 13,14 (Fig. 1).…”

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confidence: 99%

Inhibition of interaction between Staphylococcus aureus α-hemolysin and erythrocytes membrane by hydrolysable tannins: structure-related activity study

Olchowik-Grabarek

Sękowski

Bitiucki

et al. 2020

Sci Rep

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The objective of the study was a comparative analysis of the antihemolytic activity against two Staphylococcus aureus strains (8325-4 and NCTC 5655) as well as α-hemolysin and of the membrane modifying action of four hydrolysable tannins with different molecular mass and flexibility: 3,6-bis-O-di-O-galloyl-1,2,4-triO -galloyl-β-d-glucose (T1), 1,2,3,4,5-penta-O-galloyl-β-d-glucose (T2), 3-O-galloyl-1,2-valoneoyl-β-d-glucose (T3) and 1,2-di-O-galloyl-4,6-valoneoyl-β-d-glucose (T4). We showed that all the compounds studied manifested antihemolytic effects in the range of 5-50 µM concentrations. However, the degree of the reduction of hemolysis by the investigated tannins was not uniform. A valoneoyl group-containing compounds (T3 and T4) were less active. Inhibition of the hemolysis induced by α-hemolysin was also noticed on preincubated with the tannins and subsequently washed erythrocytes. In this case the efficiency again depended on the tannin structure and could be represented by the following order: T1 > T2 > T4 > T3. We also found a relationship between the degree of antihemolytic activity of the tannins studied and their capacity to increase the ordering parameter of the erythrocyte membrane outer layer and to change zeta potential. Overall, our study showed a potential of the T1 and T2 tannins as anti-virulence agents. The results of this study using tannins with different combinations of molecular mass and flexibility shed additional light on the role of tannin structure in activity manifestation. Over the past few years, a significant increase in bacterial resistance to antibiotics and transference of resistance genes from animal to human strains has become a global medical problem. Therefore, constant search for new antimicrobial agents among them being compounds of plant origin, including polyphenols, is ongoing 1,2. In addition to the antibiotic approaches of combating bacteria, anti-virulence strategies have also been considered recently. An anti-virulent strategy assumes a direct effect of compounds on virulent factors by reducing

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“…The antibacterial activity of Bacillus spp. lipopeptide was observed with other pathogens, such as S. aureus [28], Vibrio anguillarum [18], and E. clocae [8]. However, our report is the first to document the effects of lipopeptide on toxinproducing C. difficile.…”

Section: Effect Of C-1 Lipopeptide On C Difficile Morphology Cellmentioning

confidence: 51%

Mechanism of Antibacterial Activity of Bacillus amyloliquefaciens C-1 Lipopeptide toward Anaerobic Clostridium difficile

Jia

Cheng

et al. 2020

BioMed Research International

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Probiotics may offer an attractive alternative for standard antibiotic therapy to treat Clostridium difficile infections (CDI). In this study, the antibacterial mechanism in vitro of newly isolated B. amyloliquefaciens C-1 against C. difficile was investigated. The lipopeptides surfactin, iturin, and fengycin produced by C-1 strongly inhibited C. difficile growth and viability. Systematic research of the bacteriostatic mechanism showed that the C-1 lipopeptides damage the integrity of the C. difficile cell wall and cell membrane. In addition, the lipopeptide binds to C. difficile genomic DNA, leading to cell death. Genome resequencing revealed many important antimicrobial compound-encoding clusters, including six nonribosomal peptides (surfactins (srfABCD), iturins (ituABCD), fengycins (fenABCDE), bacillibactin (bmyABC), teichuronic, and bacilysin) and three polyketides (bacillaene (baeEDLMNJRS), difficidin (difABCDEFGHIJ), and macrolactin (mlnABCDEFGHI)). In addition, there were other beneficial genes, such as phospholipase and seven siderophore biosynthesis gene clusters, which may contribute synergistically to the antibacterial activity of B. amyloliquefaciens C-1. We suggest that proper application of antimicrobial peptides may be effective in C. difficile control.

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Antimicrobial activity and mechanism of Larch bark procyanidins against <italic>Staphylococcus aureus</italic>

Cited by 60 publications

References 15 publications

Antibacterial Activity and Mode of Action of Dihydromyricetin from Ampelopsis grossedentata Leaves against Food-Borne Bacteria

Antibacterial Activity and Mode of Action of Dihydromyricetin from Ampelopsis grossedentata Leaves against Food-Borne Bacteria

Inhibition of interaction between Staphylococcus aureus α-hemolysin and erythrocytes membrane by hydrolysable tannins: structure-related activity study

Mechanism of Antibacterial Activity of Bacillus amyloliquefaciens C-1 Lipopeptide toward Anaerobic Clostridium difficile

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