Antimicrobial activity and biosynthetic potential of cultivable actinomycetes associated with Lichen symbiosis from Qinghai-Tibet Plateau

Hei, Yueyu; Zhang, Hongli; Tan, Nana; Zhou, Youyou; Xin, Wei; Hu, Chenhao; Liu, Yuande; Wang, Le; Qi, Jianzhao; Gao, Jin‐Ming

doi:10.1016/j.micres.2020.126652

Cited by 25 publications

(23 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…strains showed high antimicrobial activity were inoculated into 50 ml of ISP2 medium without Agar and subjected to constant agitation for 5–7 days until the turbidity of the medium was high. Cells were concentrated by centrifugation at 10,000 g for 20 min at 4 °C, and then congealed (without supernatant) in 2 mL eppendorfs tubes at − 20 °C until used 86 . DNA extraction was performed by an automated system (Mag Purix Bacterial DNA Extraction Kit) following the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial and antioxidant activities of Streptomyces species from soils of three different cold sites in the Fez-Meknes region Morocco

Rammali

Hilali

Dari

et al. 2022

Sci Rep

View full text Add to dashboard Cite

The increasing demand for new bioactive compounds to combat the evolution of multi-drug resistance (MDR) requires research on microorganisms in different environments in order to identify new potent molecules. In this study, initial screening regarding the antimicrobial activity of 44 Actinomycetes isolates isolated from three soil samples from three different extremely cold sites in Morocco was carried out. Primary and secondary screening were performed against Candida albicans ATCC 60,193, Escherichia coli ATCC 25,922, Staphylococcus aureus ATCC 25,923, Bacillus cereus ATCC 14,579, other clinical MDR bacteria, and thirteen phytopathogenic fungi. Based on the results obtained, 11 active isolates were selected for further study. The 11microbial isolates were identified based on morphological and biochemical characters and their molecular identification was performed using 16S rRNA sequence homology. The UV–visible analysis of dichloromethane extracts of the five Streptomyces sp. Strains that showed high antimicrobial and antioxidant (ABTS 35.8% and DPPH 25.6%) activities revealed the absence of polyene molecules. GC–MS analysis of the dichloromethane extract of E23-4 as the most active strain revealed the presence of 21 volatile compounds including Pyrrolopyrazine (98%) and Benzeneacetic acid (90%). In conclusion, we studied the isolation of new Streptomyces strains to produce new compounds with antimicrobial and antioxidant activities in a cold and microbiologically unexplored region of Morocco. Furthermore, this study has demonstrated a significant (P < 0.0001) positive correlation between total phenolic and flavonoid contents and antioxidant capacity, paving the way for the further characterization of these Streptomyces sp. isolates for their optimal use for anticancer, antioxidant, and antimicrobial purposes.

show abstract

Section: Methodsmentioning

confidence: 99%

Antimicrobial and antioxidant activities of Streptomyces species from soils of three different cold sites in the Fez-Meknes region Morocco

Rammali

Hilali

Dari

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Their broad spectrum of activity suggests potential for the development of a therapeutic strategy to control antimicrobial resistance. In a recent study, some natural compounds belonging to pyrrolidine, anthracyclines, and indole derivatives identified from the actinobacteria, Streptomyces , were shown to have inhibitory activity on HIV-1 reverse transcriptase, indicating that actinobacteria are promising sources of biological active metabolites, which could provide important bioactive compounds as potential antibiotic drugs (Hei et al 2021 ).…”

Section: Approaches To Combat Antimicrobial Resistance To Complement ...mentioning

confidence: 99%

Occurrence of antibiotics and bacterial resistance genes in wastewater: resistance mechanisms and antimicrobial resistance control approaches

Mutuku

Gazdag

Melegh

2022

World J Microbiol Biotechnol

View full text Add to dashboard Cite

Antimicrobial pharmaceuticals are classified as emergent micropollutants of concern, implying that even at low concentrations, long-term exposure to the environment can have significant eco-toxicological effects. There is a lack of a standardized regulatory framework governing the permissible antibiotic content for monitoring environmental water quality standards. Therefore, indiscriminate discharge of antimicrobials at potentially active concentrations into urban wastewater treatment facilities is rampant. Antimicrobials may exert selective pressure on bacteria, leading to resistance development and eventual health consequences. The emergence of clinically important multiple antibiotic-resistant bacteria in untreated hospital effluents and wastewater treatment plants (WWTPs) has been linked to the continuous exposure of bacteria to antimicrobials. The levels of environmental exposure to antibiotics and their correlation to the evolution and spread of resistant bacteria need to be elucidated to help in the formulation of mitigation measures. This review explores frequently detected antimicrobials in wastewater and gives a comprehensive coverage of bacterial resistance mechanisms to different antibiotic classes through the expression of a wide variety of antibiotic resistance genes either inherent and/or exchanged among bacteria or acquired from the reservoir of antibiotic resistance genes (ARGs) in wastewater systems. To complement the removal of antibiotics and ARGs from WWTPs, upscaling the implementation of prospective interventions such as vaccines, phage therapy, and natural compounds as alternatives to widespread antibiotic use provides a multifaceted approach to minimize the spread of antimicrobial resistance.

show abstract

“…They have also revealed that lichens are in fact better described as micro-communities with multiple additional components, such as basidiomycete yeasts and other bacteria, mainly Proteobacteria and Actinobacteria [ 299 , 300 , 301 ]. Lichens are widespread in nature and can be found in multiple habitats, including extreme environments [ 302 , 303 ], and it is thought the mycobiont offers structural support and protection against abiotic stress, whereas the photobiont offers nutrient acquisition through photosynthesis and nitrogen fixation [ 299 ].…”

Section: Lichensmentioning

confidence: 99%

“…These observations, alongside other untargeted metabolomics’ studies on lichens strongly suggest that some secondary metabolites can be specific to symbiotic relationships and that exploring these niches can expand the chemical diversity of this kind of metabolites [ 296 , 320 ]. This is true not only of lichens or their main components, as further work on lichen-like symbioses [ 321 ] or other lichen associated microorganisms, mainly Actinobacteria [ 303 , 304 , 322 ], has revealed rich, secondary metabolite-driven interactions [ 323 ] and promising leads for clinical use such as the enedine antibiotic uncialamycin ( Figure 13 ).…”

Section: Lichensmentioning

confidence: 99%

Beyond Soil-Dwelling Actinobacteria: Fantastic Antibiotics and Where to Find Them

Santos‐Aberturas

Vior

2022

Antibiotics

View full text Add to dashboard Cite

Bacterial secondary metabolites represent an invaluable source of bioactive molecules for the pharmaceutical and agrochemical industries. Although screening campaigns for the discovery of new compounds have traditionally been strongly biased towards the study of soil-dwelling Actinobacteria, the current antibiotic resistance and discovery crisis has brought a considerable amount of attention to the study of previously neglected bacterial sources of secondary metabolites. The development and application of new screening, sequencing, genetic manipulation, cultivation and bioinformatic techniques have revealed several other groups of bacteria as producers of striking chemical novelty. Biosynthetic machineries evolved from independent taxonomic origins and under completely different ecological requirements and selective pressures are responsible for these structural innovations. In this review, we summarize the most important discoveries related to secondary metabolites from alternative bacterial sources, trying to provide the reader with a broad perspective on how technical novelties have facilitated the access to the bacterial metabolic dark matter.

show abstract

Antimicrobial activity and biosynthetic potential of cultivable actinomycetes associated with Lichen symbiosis from Qinghai-Tibet Plateau

Cited by 25 publications

References 64 publications

Antimicrobial and antioxidant activities of Streptomyces species from soils of three different cold sites in the Fez-Meknes region Morocco

Antimicrobial and antioxidant activities of Streptomyces species from soils of three different cold sites in the Fez-Meknes region Morocco

Occurrence of antibiotics and bacterial resistance genes in wastewater: resistance mechanisms and antimicrobial resistance control approaches

Beyond Soil-Dwelling Actinobacteria: Fantastic Antibiotics and Where to Find Them

Contact Info

Product

Resources

About