Antimicrobial activity and biofilm inhibition of riparins I, II and III and ultrastructural changes in multidrug-resistant bacteria of medical importance

Júnior, Jorge Belém Oliveira; Silva, Everton Morais da; Veras, Dyana Leal; Ribeiro, Karla Raíza Cardoso; Freitas, Catarina Fernandes de; Lima, Fernanda Cristina Gomes de; Gutierrez, Stanley Juan Chávez; Câmara, Celso A.; Barbosa-Filho, José Maria; Alves, Luíz Carlos; Brayner, Fábio André

doi:10.1016/j.micpath.2020.104529

Cited by 12 publications

(4 citation statements)

References 24 publications

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“…In the literature, we found no studies describing the antifungal and antibiofilm activity of RIP3 against dermatophytes. However, we found that RIP3 showed better antibacterial activity against Gram-positive than Gram-negative bacteria [ 20 ]. In a previous study, S. aureus strains were more sensitive to RIP3 than Escherichia coli strains [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

“…RIP3 exhibited antimicrobial activity in multidrug-resistant S. aureus and Acinetobacter baumannii and promoted ultrastructural change. In addition, RIP3 showed potential antibiofilm produced by S. aureus [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

“…Due to this observation, we searched for studies that analyzed the toxicity of RIP3. RIP3 induced cytotoxicity and hemolysis similar to oxacillin and meropenem, two antimicrobials used in clinical practice [ 20 ]. In a study performed with embryos of Gallus gallus , morphological alterations were observed in all the tested concentrations of RIP3.…”

Section: Discussionmentioning

confidence: 99%

“…In this sense, pharmacological investigations of natural products with multiple biological activities of medicinal interest have increased the importance of developing multi-targeted drugs [ 16 ]. For example, several pharmacological activities have been described for RIP3, such as potential antianxiety [ 17 , 18 ], antidepressant [ 19 ], and antibacterial activity [ 20 , 21 ]. However, to the best of our knowledge, there has been no report of biological studies on RIP3 against dermatophytes.…”

Section: Introductionmentioning

confidence: 99%

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Antifungal and Antibiofilm Activity of Riparin III against Dermatophytes

Silva

Rocha

Silva

et al. 2023

JoF

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The ability of dermatophytes to develop biofilms is possibly involved in therapeutic failure because biofilms impair drug effectiveness in the infected tissues. Research to find new drugs with antibiofilm activity against dermatophytes is crucial. In this way, riparins, a class of alkaloids that contain an amide group, are promising antifungal compounds. In this study, we evaluated the antifungal and antibiofilm activity of riparin III (RIP3) against Trichophyton rubrum, Microsporum canis, and Nannizzia gypsea strains. We used ciclopirox (CPX) as a positive control. The effects of RIP3 on fungal growth were evaluated by the microdilution technique. The quantification of the biofilm biomass in vitro was assessed by crystal violet, and the biofilm viability was assessed by quantifying the CFU number. The ex vivo model was performed on human nail fragments, which were evaluated by visualization under light microscopy and by quantifying the CFU number (viability). Finally, we evaluated whether RIP3 inhibits sulfite production in T. rubrum. RIP3 inhibited the growth of T. rubrum and M. canis from 128 mg/L and N. gypsea from 256 mg/L. The results showed that RIP3 is a fungicide. Regarding antibiofilm activity, RIP3 inhibited biofilm formation and viability in vitro and ex vivo. Moreover, RIP3 inhibited the secretion of sulfite significantly and was more potent than CPX. In conclusion, the results indicate that RIP3 is a promising antifungal agent against biofilms of dermatophytes and might inhibit sulfite secretion, one relevant virulence factor.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%