Antimicrobial activities of the methanol extract and compounds from Artocarpus communis (Moraceae)

Abstract

BackgroundArtocarpus communis is used traditionally in Cameroon to treat several ailments, including infectious and associated diseases. This work was therefore designed to investigate the antimicrobial activities of the methanol extract (ACB) and compounds isolated from the bark of this plant, namely peruvianursenyl acetate C (1), α-amyrenol or viminalol (2), artonin E (4) and 2-[(3,5-dihydroxy)-(Z)-4-(3-methylbut-1-enyl)phenyl]benzofuran-6-ol (5).MethodsThe liquid microdilution assay was used in the determination of the minimal inhibitory concentration (MIC) and the minimal microbicidal concentration (MMC), against seven bacterial and one fungal species.ResultsThe MIC results indicated that ACB as well as compounds 4 and 5 were able to prevent the growth of all tested microbial species. All other compounds showed selective activities. The lowest MIC value of 64 μg/ml for the crude extract was recorded on Staphylococcus aureus ATCC 25922 and Escherichia coli ATCC 8739. The corresponding value of 32 μg/ml was recorded with compounds 4 and 5 on Pseudomonas aeruginosa PA01 and compound 5 on E. coli ATCC 8739, their inhibition effect on P. aeruginosa PA01 being more than that of chloramphenicol used as reference antibiotic.ConclusionThe overall results of this study provided supportive data for the use of A. communis as well as some of its constituents for the treatment of infections associated with the studied microorganisms.

Prenylated Flavonoids and Resveratrol Derivatives Isolated from Artocarpus communis with the Ability to Overcome TRAIL Resistance

“…23 However, to the best of our knowledge, this is the first study on the ability of artonin E (5) to overcome TRAIL resistance.…”

Stability and biological activity of Merlot (Vitis vinifera) grape pomace phytochemicals after simulated in vitro gastrointestinal digestion and colonic fermentation

“…Six Gram-negative bacteria (Escherichia coli 1, E. coli 2, Klebsiella pneumoniae 1, K. pneumoniae 2, Morganella morganii and Pseudomonas aeruginosa isolated from urine and expectoration) and four Gram-positive bacteria (MRSA-methicillin-resistant Staphylococcus aureus, MSSA-methicillin-susceptible Staphylococcus aureus, Listeria monocytogenes and Enterococcus faecalis) were used to screen the antibacterial activity of the lyophilized extract. MIC determinations were performed by the microdilution method and the rapid p-iodonitrotetrazolium chloride (INT) colorimetric assay following the methodology suggested by Kuete, Ango, et al (2011) & Kuete, Justin, et al (2011 with some modifications. MIC was defined as the lowest extract concentration that prevented this change and exhibited inhibition of bacterial growth.…”

Effects of in vitro digestion and in vitro colonic fermentation on stability and functional properties of yerba mate (Ilex paraguariensis A. St. Hil.) beverages

“…Seven Gram-negative bacteria (Escherichia coli, E. coli ESBL (extended spectrum of beta-lactamase), Klebsiella pneumoniae, K. pneumoniae ESBL, Morganella morganii, Pseudomonas aeruginosa and Acinetobacter baumannii isolated from urine and expectoration) and five Gram-positive bacteria (MRSA -methicillin-resistant Staphylococcus aureus, MSSA -methicillinsusceptible Staphylococcus aureus, Staphylococcus aureus, Listeria monocytogenes and Enterococcus faecalis) were used to screen the antibacterial activity of the lyophilized extract. Minimum inhibitory concentration (MIC) determinations were performed by the microdilution method and the rapid p-iodonitrotetrazolium chloride (INT) colorimetric assay (Kuete et al, 2011a(Kuete et al, , 2011b) with some modifications. MIC was defined as the lowest extract concentration that prevented this change and exhibited inhibition of bacterial growth.…”