Introduction
The burden of multiple drug resistance in human pathogens has necessitated the search for and development of antimicrobial agents with a wide range of structural classes and potentials to selectively act on the several mechanisms of actions exhibited by the pathogens. However, most synthetic antimicrobial agents have been linked with adverse side effects and high costs, furthering the need to explore more options.
Syzygium cumini
,
Moringa oleifera
, and
Tinospora cordifolia
are three medicinal plants used in traditional medicine systems for various infectious diseases. They contain various phytochemicals that exhibit antimicrobial activities against various bacteria, fungi, and parasites. The mechanisms of their antimicrobial action may involve the disruption of microbial cell walls and membranes, the inhibition of microbial enzyme and biofilm formation, the modulation of microbial gene expression and quorum sensing, and the induction of microbial cell death. Therefore, the present study evaluated the potentials of aqueous and ethanol extracts of
S. cumini
,
M. oleifera
, and
T. cordifolia
in managing infections as measured by their inhibitory effects on species.
Materials and method
Syzygium cumini
,
M. oleifera
, and
T. cordifolia
were obtained and authenticated, and their aqueous and ethanol extracts were prepared. The antibacterial properties of the aqueous and ethanol extracts were examined. In addition to broth microdilution and biofilm development experiments, we also employed disk diffusion and agar-well diffusion techniques. The inocula of various species, including
krusei
,
parapsilosis
,
utilis
,
albicans
, and
glabrata
, were prepared for these assays. The synergistic effect of plant extracts with fluconazole was also evaluated.
Results
Syzygium cumini
,
M. oleifera
, and
T. cordifolia
emerge as promising sources for the development of effective and sustainable antimicrobial interventions. Interestingly, the aqueous and ethanol extracts were effective against the selected species. Also, the synergistic combination of plant extracts with fluconazole was observed to triple the potency of the extracts. Furthermore, the potency of the plant extract as an antifungal and synergistic agent was ranked as
S. cumini
>
M. oleifera
>
T. cordifolia
. Conclusively, the plant extracts are effective in the management of opportunistic fungal infections.