Antimicrobial Activities of Lactic Acid Bacteria Strains Isolated from Nile Tilapia Intestine (Oreochromis niloticus)

Zapata, Ana; Lara-Flores, Maurilio

doi:10.5296/jbls.v4i1.2408

Cited by 17 publications

(16 citation statements)

References 23 publications

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“…These strains were similarly isolated from the gut of the Nile tilapia (Zapata and Lara-Flores, 2012). The findings are in agreement with (Ringø et al, 1997) who found that 10% microbiota population in Artic charr (Salvelinus aplinus L.) was lactic acid bacteria.…”

Section: Discussionsupporting

confidence: 80%

Isolation and identification of potential probiotic bacteria on surfaces of Oreochromis niloticus and Clarias gariepinus from around Kampala, Uganda

Kato¹,

Kabarozi²,

Majalija³

et al. 2016

Afr. J. Microbiol. Res.

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Increased fish mortality due to infections has forced most farmers to resort the use of chemotherapeutic agents especially antibiotics. The continued use of these drugs in aquaculture is becoming limited as pathogens develop resistance and infer unpredicted long term public health effects. More research efforts are building to identify alternative disease prevention methods, among which the use of probiotics has been proposed. Therefore, the purpose of this study was to identify potential probiotics on surfaces of tilapia and catfish in areas around Kampala. Tilapia and catfish samples were aseptically collected from selected cages, ponds, tanks and hatcheries around Kampala, including Lake Victoria. The skin of fish was swabbed and then cultured on both general purpose and selective media. Probiotic screening was done using the agar spot method. Results revealed complete growth across all samples. The total microbial load was highest in fish from lakes (1000±9.6×10 5 cfu) and cages (1001±5.0×10 5 cfu). In all cases tilapia fish was significantly (p<0.0001) more contaminated than catfish. Out of the three strains of probiotics isolated, only Lactobacillus spp and Lactococcus spp showed antibacterial activity against pathogenic bacteria. The activity of Lactobacillus spp was significantly high (p< 0.0001) with Streptococcus spp (16.5±0.2 mm). Lactobacillus spp inhibited growth of only Proteus spp (5±0.2 mm). Our study shows that Lactobacillus spp and Lactococcus spp isolated from tilapia and catfish possess probiotic activity against a number of pathogenic bacteria. Our findings have significant implications for subsequent probiotic formulation and testing in aquaculture.

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Section: Discussionsupporting

confidence: 80%

Isolation and identification of potential probiotic bacteria on surfaces of Oreochromis niloticus and Clarias gariepinus from around Kampala, Uganda

Kato¹,

Kabarozi²,

Majalija³

et al. 2016

Afr. J. Microbiol. Res.

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“…A number of reports have confirmed that LAB are normal flora in gastrointestinal tract of healthy animals like mammals and aquaculture animals including fish [ 16 ] with no harmful effects [ 17 ]. Probiotics improve intestinal microflora with health beneficial efficacy protecting them against infections by stimulating the immune system, as well as alleviate lactose intolerance, reduce blood cholesterol levels, improve weight gain and feed conversion ratio [ 18 – 21 ]. Isolation of LAB from variety of samples has raised debate over the safety of probiotic bacteria and whether or not the bacteria are actually infectious [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization and pharmacological potential of Lactobacillus sakei 1I1 isolated from fresh water fish Zacco koreanus

et al. 2016

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BackgroundThere are still a large variety of microorganisms among aquatic animals which have not been explored for their pharmacological potential. Hence, present study was aimed to isolate and characterize a potent lactic acid bacterium from fresh water fish sample Zacco koreanus, and to confirm its pharmacological potential.MethodsIsolation of lactic acid bacteria (LAB) from fresh water fish samples was done using serial dilution method. Biochemical identification and molecular characterization of selected LAB isolate 1I1, based on its potent antimicrobial efficacy, was accomplished using API kit and 16S rRNA gene sequencing analysis. Further, 1I1 was assessed for α-glucosidase and tyrosinase inhibitory potential as well as antiviral efficacy against highly pathogenic human influenza virus H1N1 using MDCK cell line in terms of its pharmacological potential.ResultsHere, we first time report isolation as well as biochemical and molecular characterization of a lactic acid bacterium Lactobacillus sakei 1I1 isolated from the intestine of a fresh water fish Z. koreanus. As a result, L. sakei 1I1 exhibited potent antimicrobial effect in vitro, and diameter of zones of inhibition of 1I1 against the tested pathogens was found in the range of 13.32 ± 0.51 to 23.16 ± 0.32 mm. Also L. sakei 1I1 at 100 mg/ml exhibited significant (p < 0.05) α–glucosidase and tyrosinase inhibitory activities by 60.69 and 72.59 %, in terms of its anti-diabetic and anti-melanogenic potential, respectively. Moreover, L. sakei 1I1 displayed profound anti-cytopathic effect on MDCK cell line when treated with its ethanol extract (100 mg/ml), confirming its potent anti-viral efficacy against H1N1 influenza virus.ConclusionsThese findings reinforce the suggestions that L. sakei 1I1 isolated from the intestine of fresh water fish Z. koreanus might be a candidate of choice for using in pharmacological preparations as an effective drug.

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“…Based on antibacterial activity Bacillus sp., Enterobacter sp., Aeromonas sp., (G1), (G3), (G4) has shown higher inhibition against pathogens Staphylococcus aureus, Shigella sonnei, Enterococcus faecalis, Pseudomonas aeruginosa and Klebsilla pneumoniae. Zapata et al (2013) reported that Lactic acid bacterial strains isolated from Nile Tilapia intestine showed the high ability to inhibit the growth of freshwater fish pathogen particularly Vibrio sp. and Mycobacterium sp.…”

Section: Resultsmentioning

confidence: 99%

Isolation and Characterization of Bacteria from the Gut of Blue Gourami (Trichogaster tricopters) and its Role on Growth

Deepika¹,

Sivakumar²,

Rajan³

2019

J Pure Appl Microbiol

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Five variant groups were isolated from the gut of Blue gourami Trichogaster tricopterus through serial dilution, identified by using biochemical tests and bacteria were Bacillus sp., (G1) Pseudomonas sp., (G2) Enterobacter sp., (G3) Aeromonas sp., (G4) and Escherichia sp., (G5). Based on enzymatic productivity (Amylase, Cellulase, Lipase, and Protease) and antimicrobial activity Bacillus sp., Enterobacter sp., and Aeromonas sp., and mass multiplied using nutrient broth. Four different feeds such as feed I (Control), II (1ml Bacillus sp.,) III (1ml each of Bacillus sp. and Enterobacter sp.) and IV (1ml each of Bacillus sp., Enterobacter sp. and Aeromonas sp.,) were prepared. Feed utilization parameters of blue gourami Trichogaster tricopterus were studied after 21 days of rearing and all feed utilization parameters were higher in feed IV.

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Antimicrobial Activities of Lactic Acid Bacteria Strains Isolated from Nile Tilapia Intestine (Oreochromis niloticus)

Cited by 17 publications

References 23 publications

Isolation and identification of potential probiotic bacteria on surfaces of Oreochromis niloticus and Clarias gariepinus from around Kampala, Uganda

Isolation and identification of potential probiotic bacteria on surfaces of Oreochromis niloticus and Clarias gariepinus from around Kampala, Uganda

Characterization and pharmacological potential of Lactobacillus sakei 1I1 isolated from fresh water fish Zacco koreanus

Isolation and Characterization of Bacteria from the Gut of Blue Gourami (Trichogaster tricopters) and its Role on Growth

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