Antimicrobial activities of <i>Tephrosia vogelii</i> against selected pathogenic fungi and bacteria strains

Mlozi, Stephano Hanolo; Mmongoyo, Juma A.; Chacha, Musa

doi:10.1080/21501203.2019.1705929

Cited by 10 publications

(6 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Also, this study on phytochemical screening revealed the absence of alkaloids and anthraquinones suggesting that these medicinal properties of Tephrosia vogelii found particularly in Tanzania may be branded mainly by tannins, steroids, terpenes, flavonoids and glycosides. On the other hand, the chemical nature of the phytochemicals analysed from this medicinal plant clearly coincides with the antimicrobial activities and ectoparasitic activities reported in the previous studies (Alamgir, 2017;Arif et al, 2011;Badri et al, 2017;Kenechukwu et al, 2012;Irawan et al, 2018;Makoshi & Arowolo, 2011;Mbunde et al, 2016;Mlozi et al, 2020;Murtaza et al, 2015;Nneka & Jude, 2012;Okpogba et al, 2019;Omodamiro & Amechi, 2013;Pietta, 2000;Priya et al, 2014;Ramawat & Mérillon, 2013;Sahu et al, 2014;Samuel et al, 2019;Sasidharan et al, 2011;Singh et al, 2017;Swamy et al, 2015;Trakranrungsie, 2011).…”

Section: Phytochemical Investigationsupporting

confidence: 86%

“…These pieces of evidence generated from our GC-MS analysis and literature suggest that among other, the presence of identified flavonoids, sesquiterpenes and fatty acids contributes largely to the antimicrobial activities (Mlozi et al, 2020). Moreover, these phytocompounds sustenance reported ethnopharmacological possessions of Tephrosia vogelii.…”

Section: Phytochemical Investigationmentioning

confidence: 63%

“…In that regard, it is important to investigate and analyze phytocomponents of medicinal plants in order to discern the chemical functionalities as well as the possible mode of action of bioactive compounds. Following antimicrobial activities of extracts worked it was worth identifying phytochemicals found in methanolic leaf and root extracts (Mlozi et al, 2020).…”

Section: Phytochemical Investigationmentioning

confidence: 99%

See 2 more Smart Citations

Antimicrobial investigation of Tephrosia Vogelii hook.f from Hai District in Tanzania towards development of antifungal agents for topical application

Hanolo

View full text Add to dashboard Cite

Infectious diseases such as fungal diseases are the global health problem. Moreover, antimicrobial resistance to modern fungicides and antibiotics is even perplexing in the health settings. Such situation needs serious attention to search for alternative antimicrobial agents. Thus, this study aimed to investigate antimicrobial activity of Tephrosia vogelii Hook.f found in Tanzania and develop antifungal agents therefrom. The n-hexane, dichloromethane and methanolic extracts from leaves and roots of Tephrosia vogelii were evaluated for antimicrobial activity against Candida albicans (ATCC 90028), Cryptococcus neoformans (clinical isolate), Staphylococcus aureus (ATCC25923), Escherichia coli (ATCC29953), Klebsiella pneumoniae (ATCC 700603) and Salmonella typhi (NCTC 8385). A serial dilution method using the sterilized 96 wells of polystyrene microliter plates were used to determine the minimum inhibitory concentrations (MIC) of extracts. The in vivo toxicity of methanolic leaf and root extracts was evaluated using albino rats. Extracts doses of 600, 1200, 2000 and 5000 mg/kg body weight were evaluated for lethality test while doses of 600, 1200 and 2000 mg/kg body weight subjected for sub-acute toxicity test. Gas Chromatography-Mass Spectrometry (GC-MS) technique was employed to appraise phytochemical compounds present. Antifungal agent for topical application was formulated and evaluated through in vitro and in vivo test to establish its effectiveness. The study results revealed that n-hexane and dichloromethane extracts exhibited the lowest activity. Equally, methanolic root and leaf extracts exhibited high activity at MICs ranging from 0.625 - 5.0 mg/mL against assayed pathogens. Phytochemical screening of both methanolic leaf and root extracts revealed the presence of tannins, flavonoids, terpenes, fatty acids and saponins. The GC-MS analysis unveiled the presence of ten phytocompounds. Five compounds; demethylmunduserone sumatrol, munduserone, hexadecanoic acid and hexadecanoic acid, methyl ester are new and this is the first time to be reported from Tephrosia vogelii. Furthermore, toxicity evaluation revealed that methanolic leaf and root extracts of Tephrosia vogelii were not deleterious at highest dose of 5000 mg/kg body weight. Though there was no mortality recorded, histopathological examination revealed toxicity indices due to vacuolation and necrosis in hepatocytes under treatment of methanolic root extracts of dose 2000 mg/kg body weight. The herbal cream prototype (CBTV3) was formulated, and it exhibited antimicrobial activity through in vitro and in vivo tests. The development of herbal cream prototype from Tephrosia vogelii Hook.f is hereby reported for the first time.

show abstract

Section: Phytochemical Investigationsupporting

confidence: 86%

Section: Phytochemical Investigationmentioning

confidence: 63%

Section: Phytochemical Investigationmentioning

confidence: 99%

See 1 more Smart Citation

Antimicrobial investigation of Tephrosia Vogelii hook.f from Hai District in Tanzania towards development of antifungal agents for topical application

Hanolo

View full text Add to dashboard Cite

show abstract

“…In serial dilution experiments (1024–32 μg/mL), RHA did not show antimicrobial activity against investigated bacteria, yeast, or filamentous fungi. Despite studies confirming the antimicrobial activity of alcoholic extracts from other Tephrosia species, it has an MIC > 2.5 mg/mL [ 116 , 117 ], reaching MIC = 200 mg/mL [ 118 ]. These results suggest that the antimicrobial formulations of Tephrosia must be administered in high concentrations for the manifestation of their activity.…”

Section: Discussionmentioning

confidence: 99%

Tephrosia toxicaria (Sw.) Pers. extracts: Screening by examining aedicidal action under laboratory and field conditions along with its antioxidant, antileishmanial, and antimicrobial activities

et al. 2023

View full text Add to dashboard Cite

An increase in the incidence of arboviral, microbial and parasitic infections, and to disorders related to oxidative stress has encouraged the development of adjuvant therapies based on natural formulations, such as those involving plant extracts. Thus, to expand the repertoire of the available therapeutic options, this study aimed to describe the versatility of Tephrosia toxicaria (Sw.) (Pers., 1807) extracts for the control of arbovirus vectors, as well as their antioxidant, antileishmanial, and antimicrobial potential. Among the aqueous and hydroethanolic extracts obtained, the hydroethanolic extract from roots (RHA) was identified as the most active larvicide extract demonstrating, respectively, the lowest lethal concentration (mg/mL) for 50%, 90% and 99% of Aedes aegypti (L., 1762) and Aedes albopictus (S., 1894) larvae, observed at 24 h (0.33, 0.84 and 1.80; 0.32, 0.70 and 1.32) and 48 h (0.17, 0.51 and 1.22; 0.26, 0.47 and 0.78) post-exposure. Field assays revealed that RHA (0.84 mg/mL) is a potential oviposition deterrent, reducing egg-laying by approximately 90%. RHA (0.1 mg/mL) also exhibited antioxidant activity for the following tests: total antioxidant capacity (286.86 mg AAE/g), iron (87.16%) and copper (25.64%) chelation, and superoxide scavenging (10%). In the cell culture assays, RHA (0.1 mg/mL) promoted regeneration of metabolic activity (92% cell viability) in cells exposed to oxidative stress. Furthermore, RHA displayed weak antileishmanial activity (IC50 = 3.53 mg/mL) against Leishmania amazonensis and not exhibit antimicrobial activity. The extraction favored the concentration of carbohydrates in RHA, in addition to lectins and protease inhibitors, with molecular masses estimated between 10 and 24 kDa. Cytotoxicity and phytotoxicity analyses of RHA suggested its biosecurity. Thus, RHA is a multivalent extract with insecticide and antioxidant properties at low and safe concentrations. However, others studies on its indirect toxic effects are ongoing to ensure the complete safety of RHA.

show abstract

“…It has also been used traditionally in fishing activities and as traditional pesticides in local agricultural activities for many decades [17][18][19]. The pharmacological study of methanolic extracts of T. vogelii revealed its antimicrobial activities [16,20]. The pharmacological studies on bioactive compounds such as rotenoids and steroids from T. vogelii revealed the potentiality of such compounds for development of pesticides and insecticide [11][12][13][14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

The in vivo toxicity evaluation of leaf and root methanolic extracts of Tephrosia vogelii Hook.f using animal model

2020

Self Cite

View full text Add to dashboard Cite

Background Traditionally, herbal medicines are commonly used to cure several diseases since immemorial of human life. Nevertheless, the safety of some traditionally used medicinal plants is uncertain. Since Tephrosia vogelii Hook.f is a traditionally used medicinal plant, the effects of its extracts were evaluated on lethality (LD50) and sub-acute toxicity in this study. Methods Phytochemistry screening and an in vivo toxicity evaluation of leaf and root methanolic extracts of T. vogelii using laboratory albino rats were conducted. Methanolic extracts of doses 600, 1200, 2000 and 5000 mg/kg body weights were administered single dose in rats to observe deaths within 72 h in order to determine the LD50. Methanolic extracts doses of 600, 1200 and 2000 mg/kg body weights were consecutively administered for 14 days in order to evaluate sub-acute toxicity. Results Tannins, steroids, terpenoids, flavonoids and saponins were identified in the phytochemical screening. The LD50 experiments revealed zero deaths of rats for the administered doses, 600 to 5000 mg/kg body weight. Histopathological examination of liver and kidney for sub-acute toxicity test showed safety at all doses except root methanolic extracts dose of 2000 mg/kg which exhibited necrosis and vacuolation of liver cells on the 14th day. Nonetheless, hepatic necrosis and hepatic vacuolation disappeared upon time elongation without dose administration to 28th day. Conclusion The conducted toxicity evaluation of methanolic leaf and root extracts in albino rats revealed no deleterious effects, henceforth, suggesting that T. vogelii could be safe to users using it as a medicinal plant.

show abstract

Antimicrobial activities of Tephrosia vogelii against selected pathogenic fungi and bacteria strains

Cited by 10 publications

References 17 publications

Antimicrobial investigation of Tephrosia Vogelii hook.f from Hai District in Tanzania towards development of antifungal agents for topical application

Antimicrobial investigation of Tephrosia Vogelii hook.f from Hai District in Tanzania towards development of antifungal agents for topical application

Tephrosia toxicaria (Sw.) Pers. extracts: Screening by examining aedicidal action under laboratory and field conditions along with its antioxidant, antileishmanial, and antimicrobial activities

The in vivo toxicity evaluation of leaf and root methanolic extracts of Tephrosia vogelii Hook.f using animal model

Contact Info

Product

Resources

About