Antimicrobial Activities and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry of Bacillus Isolates from the Marine Sponge Aplysina aerophoba

Pabel, Christian T.; Vater, Joachim; Wilde, Christopher; Franke, P.; Hofemeister, J.; Adler, Barbara; Bringmann, Gerhard; Hacker, Jörg; Hentschel, Ute

doi:10.1007/s10126-002-0088-8

Cited by 98 publications

(69 citation statements)

References 37 publications

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“…These mass data correspond well to those determined by other authors indicating the presence of pumilacidin A-E series (Kalinovskaya et al, 2002;Pabel et al, 2003) (Figure 4).…”

Section: Mass Spectrometrysupporting

confidence: 91%

“…The production of bioactive compounds by Bacillus species is well established (Korzybski et al, 1978;Naruse et al, 1990;Munimbazi & Bullerman, 1998). These classes of bioactive compounds act as antifungal peptides, antifungal lipopeptides and antimicrobial polypeptides (Pabel et al, 2003). To characterize antimicrobial activity of B. pumilus MAIIIM4a, the isolate was tested against three phytopathogenic fungi: R. solani, P. aphanidermatum and S. rolfsii.…”

Section: Discussionmentioning

confidence: 99%

“…In order to colonize the plant and compete with other microorganisms, they produce many enzymes and toxins (Lima et al, 2005). Also, a variety of secondary metabolites, including enzymes, antibiotics (Pleban et al, 1997), anticancer (Stierle et al, 1993), anti-inflammatory (Trischman et al, 1994), antifungal (Korzybski et al, 1978), and biological control agents (Hallmann et al, 1997) have been isolated from endophytic microorganisms. To date, many promising endophytic bacteria have been reported as biocontrol candidates against plant pathogens.…”

Section: Introductionmentioning

confidence: 99%

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Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a

Melo

Fiore

Moraes³

et al. 2009

Sci. agric. (Piracicaba, Braz.)

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In the search for new organisms and new secondary metabolites, a study was conducted to evaluate the diversity of endophytic bacteria from ethnovarieties of cassava cultivated by Brazilian Amazon Indian tribes and also to study the secondary metabolites produced by a Bacillus pumilus strain. Sixty seven cassava endophytic bacteria were subjected to 16S rRNA sequencing and FAME analysis. The bacterial profile revealed that 25% of all endophytic isolates belonged to the genus Bacillus. The isolate B. pumilus MAIIIM4a showed a strong inhibitory activity against the fungi Rhizoctonia solani, Pythium aphanidermatum and Sclerotium rolfsii. Secondary metabolites of this strain were extracted using hexane, dichloromethane and ethyl acetate. Extracts were subjected to bioautography and LC/MS analysis, which allowed the identification of pumilacidin, an antifungal compound produced by B. pumilus MAIIIM4a. The bacterial endophytic localization was confirmed by cassava cell tissue examination using scanning electron microscopy. Key words: ESI-MS/MS, pumilacidin, endophytic bacteria, bioautography COMPOSTO ANTIFÚNGICO PRODUZIDO PELO ENDÓFITO DE MANDIOCA Bacillus pumilus MAIIIM4ARESUMO: Na busca de novos organismos e novos metabólitos secundários, um estudo foi conduzido visando avaliar a diversidade de bactérias endofíticas de etnovariedades de mandioca cultivadas por tribos indígenas da Amazônia brasileira e também para estudar metabólitos secundários produzidos por Bacillus pumilus. Sessenta e sete bactérias endofíticas de mandioca foram identificadas através do seqüenciamento do gene 16S rRNA e por meio da análise de ácidos graxos (FAME

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“…These mass data correspond well to those determined by other authors indicating the presence of pumilacidin A-E series (Kalinovskaya et al, 2002;Pabel et al, 2003) (Figure 4).…”

Section: Mass Spectrometrysupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a

Melo

Fiore

Moraes³

et al. 2009

Sci. agric. (Piracicaba, Braz.)

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“…ICMS spectra in the range below 2 kDa identify characteristic metabolites, e.g. of bacilli (Williams et al, 2002;Pabel et al, 2003) and cyanobacteria (Erhard et al,1997;Welker et al, 2002Welker et al, , 2004a, and we show here that highly characteristic peptaibol spectra can readily be obtained from microgram amounts of mycelia, thus providing characteristic metabolite fingerprints. A comparison of these fingerprints with molecular phylogenies of the species documents some correlation between types of peptaibol formed and phylogeny.…”

Section: Introductionmentioning

confidence: 61%

Intact-cell MALDI-TOF mass spectrometry analysis of peptaibol formation by the genus Trichoderma/Hypocrea: can molecular phylogeny of species predict peptaibol structures?

et al. 2007

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Peptaibols are characteristic linear a-aminoisobutyrate-containing peptides produced by certain Ascomycetes, especially of the genus Hypocrea/Trichoderma [Hypocrea and Trichoderma are the names for the teleo-and anamorph forms of the same taxon; where known to occur in nature, the teleomorph is used to name the species. To aid the inexperienced reader, both names (the less well known one in parentheses) are given at the first mention of each species.] Here we have investigated whether phylogenetic relationships within Trichoderma permit a prediction of the peptaibol production profiles. To this end, representative strains from a third (28) of the known species of Trichoderma, identified by the sequences of diagnostic genes and covering most clades of the established multilocus phylogeny of Trichoderma/Hypocrea, were investigated by intact-cell MALDI-TOF mass spectrometry. Peptaibols were detected in all strains, and some strains were found to produce up to five peptide families of different sizes. Comparison of the data with phylogenies derived from rRNA spacer regions (ITS1 and 2) and RNA polymerase subunit B (rpb2) gene sequences did not show a strict correlation with the types and sequences of the peptaibols produced, but the production of some groups of peptaibols appears to be found only in some clades or sections of the genus, which could be used for more targeted screening of novel compounds of this type. In an analysis of peptaibol structures, we have defined conserved key positions and have further identified and compared sequences of the corresponding adenylate domains within non-ribosomal peptide synthetases producing trichovirins, paracelsins and atroviridins. These phylogenies are not concordant with those of their producers Hypocrea virens, Hypocrea jecorina and Hypocrea atroviridis as obtained from ITS1 and 2, and rpb2, respectively, and therefore hint at a complex history of peptaibol diversity.

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“…Tekanan lingkungan, seperti kompetisi ruang, cahaya, dan sumber lainnya menyebabkan terjadinya keanekaragaman kimia pada berbagai organisme bentik, termasuk spons yang berfungsi sebagai pertahanan diri terhadap serangan predator (Sennet et al, 2002;Leal et al, 2012 ). Spons merupakan makroinvertebrata laut yang menjadi sumber senyawa bioaktif baru dengan berbagai potensi biomedis sebagai antitumor, antimikrobia, antivirus dan lain?lain (Pabel et al, 2003;Mayer et al, 2013).…”

Section: Pendahuluanunclassified

Bioprospeksi Spons, Karang Lunak dan Ascidian Asal Taman Nasional Laut Kepulauan Wakatobi: Antitumor dan Antioksidan

Fajarningsih

Nursid

Januar

et al. 2013

JPBKP

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ABSTRAKPenelitian ini bertujuan untuk mengetahui potensi manfaat (bioprospeksi) spons, karang lunak, dan ascidian asal Taman Nasional Laut (TNL) Kepulauan Wakatobi sebagai antitumor dan antioksidan. Sampel makroinvertebrata laut diambil dengan menggunakan scuba diving di empat stasiun pengambilan di perairan TNL Kep. Wakatobi. Sebanyak 74 sampel diuji aktivitas antitumornya terhadap 3 jenis sel lestari tumor (HeLa, MCF7, SKOV3) dengan metode Thiazolyl Blue Tetrazolium Blue (MTT) dan diuji pula aktivitas antioksidannya dengan menggunakan metode DPPH (2,2-diphenyl pikryl hidrazil). Sampel yang dapat menghambat >50% sel lestari tumor pada konsentrasi 20 g/ml dikategorikan sebagai sampel aktif antitumor. Sampel yang memiliki aktivitas penghambatan radikal bebas >50% pada konsentrasi 100 g/ml dalam uji DPPH dikategorikan sebagai sampel yang aktif antioksidan. Hasil penelitian menunjukkan bahwa jenis sel lestari tumor, 11 sampel aktif terhadap dua jenis sel lestari tumor dan 22 sampel aktif terhadap satu jenis sel lestari tumor. Dari 74 sampel yang diuji aktivitas antioksidannya, terdapat 8 sampel yang tergolong aktif (7 sampel spons dan 1 sampel karang lunak).

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Antimicrobial Activities and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry of Bacillus Isolates from the Marine Sponge Aplysina aerophoba

Cited by 98 publications

References 37 publications

Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a

Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a

Intact-cell MALDI-TOF mass spectrometry analysis of peptaibol formation by the genus Trichoderma/Hypocrea: can molecular phylogeny of species predict peptaibol structures?

Bioprospeksi Spons, Karang Lunak dan Ascidian Asal Taman Nasional Laut Kepulauan Wakatobi: Antitumor dan Antioksidan

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