Antimicrobial Action of Raphanus raphanistrum subsp.
                        sativus (radish) Extracts against Foodborne Bacteria
                    Present in Various Milk Products: A Preliminary Study

Lim, Hyun-Woo; Song, Kwang‐Young; Chon, Jung‐Whan; Jeong, Dongkwan; Seo, Kun‐Ho

doi:10.22424/jmsb.2019.37.3.187

Cited by 13 publications

(9 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Researchers in India found that white radish has powerful effects against the bacteria Agrobacterium rhizogenes (Muthusamy and Shanmugam, 2020). There was a study done in Korea that found that the White radish ethanol extract was efficient against Listeria monocytogenes, Salmonella Enteritidis, Escherichia coli, Cronobacter sakazakii, Bacillus cereus, and Staphylococcus aureus (Lim et al, 2019). Similar to these other researchs, we found that white radish tuber pieces effectively combat various bacterial and fungal strains.…”

Section: Antimicrobial Activitysupporting

confidence: 82%

Antioxidant and antimicrobial activities of White Radish

Sevindik

Onat²,

Mohammed

et al. 2023

Turkish JAF Sci.Tech.

View full text Add to dashboard Cite

Since prehistoric times, humans have relied on plants for a wide variety of needs, including sustenance, shelter, thermal regulation, and medical treatment. Plants are significant organics because of the many ways they may be put to use. The white radish (Raphanus raphanistrum subsp. sativus (L.) Domin) was tested for its antioxidant and antibacterial properties, as were its tuber and aerial portions. A soxhlet was used to extract ethanol from plant material. The antioxidant and oxidant capacities were tested with the use of Rel Assay TAS and TOS kits. The agar dilution technique was used to test for antimicrobial activity against the bacteria and fungus included in the experiment. As a result of the studies, the TAS value of the tuber extract of the plant was measured as 6.698±0.187, the TOS value as 5.609±0.245 and the OSI value as 0.084±0.002. The TAS value of the herbal aerial parts extract was 4.689±0.223, the TOS value was 3.746±0.128, and the OSI value was 0.080±0.003. Inhibition of tuber extract was observed at concentrations varying between 25-100 against bacterial strains and 200 µg/mL against fungal strains. Aerial parts extract showed inhibition against bacterial strains at concentrations varying between 100-200 and against fungus strains at concentrations ranging from 200-400 µg/mL. As a result, it was determined that the white radish plant has significant antioxidant and antimicrobial activity.

show abstract

Section: Antimicrobial Activitysupporting

confidence: 82%

Antioxidant and antimicrobial activities of White Radish

Sevindik

Onat²,

Mohammed

et al. 2023

Turkish JAF Sci.Tech.

View full text Add to dashboard Cite

show abstract

“…After drying, these peels were ground into a fine powder, and the milled parts were kept in clean plastic containers under a moisture-free condition until required for extraction. 9 Solvent extraction of radish root peel Ethanol and methanol extracts of radish root peels were prepared as described, 5 using 80% of both solvents. The Radish root peel sample was weighed (1 g) and added to 50 mL of each solvent.…”

Section: Preparation Of Radish Plant Materialsmentioning

confidence: 99%

Inhibitory Effects of Radish Peeled Root Extracts against Some Pathogenic Bacteria Suha M. Abed*, Lina Q. Yasin, Waqas S. Mahmood

2021

TJNPR

View full text Add to dashboard Cite

cell lysis.⁶ The present study was aimed at investigating the antibacterial activities of R. sativus extracts against pathogenic bacteria and detecting differences in virulence factor expression before and after exposure to the crude extracts. Materials and Methods Bacteria and cultural conditionsThe pathogenic bacteria were collected from different clinical cases including urinary tract infection, pharyngitis, otitis media, wounds, and lower respiratory tract infection using cotton swabs or universal sterile cups depending on the specimens. These samples were immediately transported to the laboratory under the supervision of physicians. Bacteria were isolated from the clinical samples and identified using microscopic, and cultural characterization. Biochemical tests under aerobic conditions were performed which included Indole test to investigate the production of indole; methyl red test to investigate sugar fermentation with acid production; Vogus-Proscauer test for acetone compound detection; utilization of citrate as a sole source of carbon and formation of sodium carbonate and urease test to indicate the hydrolysis of urea and formation of ammonium. In addition, an oxidase test was used to investigate the production of cytochrome; catalase and fermentation tests for sugar fermentation; motility screening, and coagulase enzyme tests were carried out in two ways (slide and tube methods) as described by Mahmoodn, 2019. 7 Confirmation of the identity of bacterial isolatesVitek compact technique using automated Vitek device (BioMérieux, Marcy l'Etoile France) was used to confirm the identity of bacterial isolates. Each isolate was cultured on nutrient agar and incubated overnight at 37°C. Bacteria were suspended in 2.5 mL of a 0.45% sodium chloride solution. The suspension used was adjusted to a McFarland standard of 0.5 using a Densicheck 8 . The cards were automatically filled by a vacuum device, sealed, and inserted into the

show abstract

“…Over the next years, knowledge about the antibacterial compounds contained in radish seeds was extended. The following pathogens, i.e., Streptococcus pyogenes , E. coli , Salmonella enteritidis 110, Cronobacter sakazakii KCTC 2949, B. cereus ATCC 10876 and Staphylococcus aureus ATCC 6538 are sensitive to radish metabolites ( Jadoun et al, 2016 ; Lim et al, 2019 ). The effect of red-peel radish root extract on 52 food-borne bacteria confirmed the presence of compounds with antimicrobial activity ( Jadoun et al, 2016 ; Kaymak et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Colonization of Raphanus sativus by human pathogenic microorganisms

Szymańska,

Deja-Sikora,

Sikora

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

Contamination of vegetables with human pathogenic microorganisms (HPMOs) is considered one of the most important problems in the food industry, as current nutritional guidelines include increased consumption of raw or minimally processed organic vegetables due to healthy lifestyle promotion. Vegetables are known to be potential vehicles for HPMOs and sources of disease outbreaks. In this study, we tested the susceptibility of radish (Raphanus sativus) to colonization by different HPMOs, including Escherichia coli PCM 2561, Salmonella enterica subsp. enterica PCM 2565, Listeria monocytogenes PCM 2191 and Bacillus cereus PCM 1948. We hypothesized that host plant roots containing bactericidal compounds are less prone to HPMO colonization than shoots and leaves. We also determined the effect of selected pathogens on radish growth to check host plant–microbe interactions. We found that one-week-old radish is susceptible to colonization by selected HPMOs, as the presence of the tested HPMOs was demonstrated in all organs of R. sativus. The differences were noticed 2 weeks after inoculation because B. cereus was most abundant in roots (log10 CFU – 2.54), S. enterica was observed exclusively in stems (log10 CFU – 3.15), and L. monocytogenes and E. coli were most abundant in leaves (log10 CFU – 4.80 and 3.23, respectively). The results suggest that E. coli and L. monocytogenes show a higher ability to colonize and move across the plant than B. cereus and S. enterica. Based on fluorescence in situ hybridization (FISH) and confocal laser scanning microscopy (CLSM) approach HPMOs were detected in extracellular matrix and in some individual cells of all analyzed organs. The presence of pathogens adversely affected the growth parameters of one-week-old R. sativus, especially leaf and stem fresh weight (decreased by 47–66 and 17–57%, respectively). In two-week-old plants, no reduction in plant biomass development was noted. This observation may result from plant adaptation to biotic stress caused by the presence of HPMOs, but confirmation of this assumption is needed. Among the investigated HPMOs, L. monocytogenes turned out to be the pathogen that most intensively colonized the aboveground part of R. sativus and at the same time negatively affected the largest number of radish growth parameters.

show abstract

Antimicrobial Action of Raphanus raphanistrum subsp. sativus (radish) Extracts against Foodborne Bacteria Present in Various Milk Products: A Preliminary Study

Cited by 13 publications

References 17 publications

Antioxidant and antimicrobial activities of White Radish

Antioxidant and antimicrobial activities of White Radish

Inhibitory Effects of Radish Peeled Root Extracts against Some Pathogenic Bacteria Suha M. Abed*, Lina Q. Yasin, Waqas S. Mahmood

Colonization of Raphanus sativus by human pathogenic microorganisms

Contact Info

Product

Resources

About