Antimicrobial Action of Compound 48/80 against Protozoa, Bacteria, and Fungi

Lenney, James F.; Siddiqui, Wasim A.; Schnell, Jerome V.; Furusawa, Eiichi; Read, George

doi:10.1002/jps.2600660524

Cited by 8 publications

(4 citation statements)

References 12 publications

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“…Compound 48/80 is a polycationic polymer of an organic monovalent cation,p-methoxyphenethylmethylamine. It has essentially no surfactant activity and is a relatively weak antimicrobial agent against bacteria, fungi, and protozoa (56,103). Like polymyxin (222), defensins (238), and magainins (70), it effectively liberates histamine from mast cells by a selective, noncytolytic action (56, 103).…”

Section: Compound 48/80mentioning

confidence: 99%

Agents that increase the permeability of the outer membrane.

Vaara

1992

Microbiological Reviews

1,064

638

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Section: Compound 48/80mentioning

confidence: 99%

Agents that increase the permeability of the outer membrane.

Vaara

1992

Microbiological Reviews

1,064

638

View full text Add to dashboard Cite

“…5 shows the results for compound 48/80. This compound is known to effectively liberate histamine via a selective and noncytotoxic action [45], and also to act as a weak antimicrobial agent [46,47]. Consistent with this, it effectively liberated histamine from mast cells at low concentrations, and reduced the viability of S. aureus cells at relatively high concentrations.…”

Section: K + Efflux Cell Viability Hemolysis and Histamine Releasementioning

confidence: 82%

“…At high concentrations, however, granuliberin R induced K + efflux from mast cells and bacterial cells, supporting the view that the peptide acted on anionic lipids of not only bacterial cells but mast cells during histamine secretion. Interestingly, compound 48/80, a potentially active histamine liberator, is also a weak antimicrobial agent [46,47]. In fact, compound 48/80 induced histamine release at very low concentrations, while relatively high concentrations were required to induce the efflux of K + from bacteria.…”

Section: Discussionmentioning

confidence: 99%

“…The concentration required for the efflux from mast cells was significantly lower than that needed for the efflux from bacteria. This may be attributable to a difference in susceptibility to compound 48/80 among anionic membranes in different environments, because the effects of amphiphilic compounds including 48/80 are known to differ among bacteria, despite the bacterial cytoplasmic membranes being generally rich in negatively charged lipids [5,26,47].…”

Section: Discussionmentioning

confidence: 99%

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Comparative study of the membrane-permeabilizing activities of mastoparans and related histamine-releasing agents in bacteria, erythrocytes, and mast cells

Nakao

Komagoe

Inoue

et al. 2011

Biochimica et Biophysica Acta (BBA) - Biomembranes

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The membrane-permeabilizing activities of mastoparans and related histamine-releasing agents were compared through measurements of K(+) efflux from bacteria, erythrocytes, and mast cells. Changes in bacterial cell viability, hemolysis, and histamine release, as well as in the shape of erythrocytes were also investigated. The compounds tested were mastoparans (HR1, a mastoparan from Polistes jadwagae, and a mastoparan from Vespula lewisii), granuliberin R, mast cell-degranulating peptide, and compound 48/80, as well as antimicrobial peptides, such as magainin I, magainin II, gramicidin S, and melittin. We used a K(+)-selective electrode to determine changes in the permeability to K(+) of the cytoplasmic membranes of cells. Consistent with the surface of mast cells becoming negatively charged during histamine release, due to the translocation of phosphatidylserine to the outer leaflet of the cytoplasmic membrane, histamine-releasing agents induced K(+) efflux from mast cells, dependent on their ability to increase the permeability of bacterial cytoplasmic membranes rich in negatively charged phospholipids. The present results demonstrated that amphiphilic peptides, possessing both histamine-releasing and antimicrobial capabilities, induced the permeabilization of the cytoplasmic membranes of not only bacteria but mast cells. Mastoparans increased the permeability of membranes in human erythrocytes at higher concentrations, and changed the normal discoid shape to a crenated form. The structural requirement for making the crenated form was determined using compound 48/80 and its constituents (monomer, dimer, and trimer), changing systematically the number of cationic charges of the molecules.

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Selective fungitoxicity of cyclohexylamine and related aromatic amines

Gindrat

1981

Experientia

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Antimicrobial Action of Compound 48/80 against Protozoa, Bacteria, and Fungi

Cited by 8 publications

References 12 publications

Agents that increase the permeability of the outer membrane.

Agents that increase the permeability of the outer membrane.

Comparative study of the membrane-permeabilizing activities of mastoparans and related histamine-releasing agents in bacteria, erythrocytes, and mast cells

Selective fungitoxicity of cyclohexylamine and related aromatic amines

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