Antimetastatic effects of Terminalia catappa L. on oral cancer via a down-regulation of metastasis-associated proteases

Yang, Shun‐Fa; Chen, Mu‐Kuan; Hsieh, Yih‐Shou; Yang, Jyh Bin; Zavras, Athanasios-I; Hsieh, Yih-Hsien; Su, Shih‐Chi; Kao, Te-Yu; Chen, Pen-Ni; Chu, Shu‐Chen

doi:10.1016/j.fct.2010.01.019

Cited by 100 publications

(89 citation statements)

References 37 publications

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“…Confocal laser microscopy examination reconfirmed that CTD suppressed NF-ĸB p65 translocation to nuclei ( Figure 6A) and that, also, CTD decreased the protein expression of UPA ( Figure 5E). The PI3K-AKT signaling pathways have been shown to play an important role in MMPs for UPA gene regulation, cell survival and invasion (40,41). Here, we demonstrated that CTD inhibited the protein expression of PI3K, AKT and UPA ( Figure 5B and 5E).…”

Section: Discussionmentioning

confidence: 49%

Cantharidin Impairs Cell Migration and Invasion of Human Lung Cancer NCI-H460 Cells via UPA and MAPK Signaling Pathways

Hsia

Hsiao

et al. 2016

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Abstract. Cantharidin (CTD), a component of natural mylabris (Mylabris phalerata Pallas), has been shown to have biological activities and induce cell death in many human and the results indicated that CTD inhibited the enzymatic activities of MMP-2/-9 of NCI-H460 cells. Western blotting was used to examine the protein expression of NCI-H460 cells after incubation with CTD and the results showed that CTD decreased the expression of MMP-2/-9, focal adhesion kinase (FAK), Ras homolog gene family, member A (Rho A), phospho-protein kinase B (AKT) (Thr308)(p-AKT(308)), phospho-extracellular signal-regulated kinase1/2 (p-ERK1/2), phospho-p38 mitogen-activated protein (MAP) kinase (p-p38), phospho c-Jun N-terminal kinase 1/2 (p-JNK1/2), nuclear factor-ĸB (NF-ĸB) and urokinase plasminogen activator (UPA). Furthermore, confocal laser microscopy was used to confirm that CTD suppressed the expression of NF-ĸB p65, but did not significantly affect protein kinase C (PKC) translocation in NCI-H460 cells. Based on those observations, we suggest that CTD may be used as a novel anticancer metastasis agent for lung cancer in the future.Lung cancer is the most common cancer among women and men in Taiwan and it is the major cause of death in cancerrelated diseases in the developed world. Neoplastic metastasis is a major cause of cancer-mediated death in humans (1, 2). For tumor metastasis, epithelial cancer cells have to migrate from the original primary tumor mass via breaking their cellcell contacts (adherens junctions) to form cancer mass in a new site (3, 4); thus, tumor metastasis involves cell attachment (adhension), migration and invasion in order to form a new tumor in another site of the body. Inhibiting cancer cell metastasis is one of the important steps for cancer therapy and research (5). Numerous evidence have shown that multiple factors involved in tumor metastasis, such as the activation of PI3K/Akt pathway (6), matrix metalloproteinases (MMPs)-enzymes that can degrade the extracellular matrix and 5989

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Section: Discussionmentioning

confidence: 49%

Cantharidin Impairs Cell Migration and Invasion of Human Lung Cancer NCI-H460 Cells via UPA and MAPK Signaling Pathways

Hsia

Hsiao

et al. 2016

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“…Determination of the human carcinoma cytotoxicity (MTT assay) For cell viability experiment, a microculture tetrazolium (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) colorimetric assay was performed to determine the cytotoxicity of test chemicals (Yang et al, 2010). All the human carcinoma cells were seeded in 24-well plates at a density of 5 9 10 4 cells/well and treated with test chemicals at 37°C for 24 h. After the exposure period, the media was removed, and cells were washed with phosphate buffered saline (PBS) and then incubated with 20 ll MTT (5 mg/ml) (Sigma chemical Co., St. Louis, MO, USA) for 4 h. The viable cell number per dish is directly proportional to the production of formazan, which can be measured spectrophotometrically at 563 nm following solubilization with isopropanol.…”

Section: Cell Culturementioning

confidence: 99%

Study on synthesis of thalidomide analogues and their bioactivities; inhibition on iNOS pathway and cytotoxic effects

et al. 2011

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Synthetic thalidomide analogues (compounds 1-35), including phenylphthalimide, pyridylphthalimide, aminobenzylphthalimide, and diphenylazophthalimide, were tested for their cytotoxic effects on human cancer cell lines Hep2 (Human Larynx Carcinoma Cells), HL-60 (Human Myeloid Leukemia Cells), NUGC (Human Gastric Carcinoma Cells), and HONE-1 (Human Nasopharyngeal Carcinoma Cells) because the incidence rate is more prominent in Asian countries than in Western countries. Compounds 17, 27, 28, and 35 were found to have antitumor activity in Hep2 and HL-60 cell lines. Compounds 2, 4, 15, 17, 19, 20, 23, and 27 can inhibit nitric oxide (NO) synthase activity by more than 90%. These thalidomide analogues were found to be potent inducible nitric oxide synthase (iNOS) inhibitors, and the iNOS inhibiting potential of compounds 17 and 27 might be an advantage for anticancer therapy. In conclusion, inhibition of NO synthesis is a new development in cancer therapy for now and in the future. We modified the structures of the thalidomide analogues to have a stronger anticancer effect and a good therapeutic effect.

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“…MMP-2 and MMP-9 are members of a unique family of zinc-binding endopeptidases that are thought to play an essential role in the process of cancer angiogenesis, invasion, migration, and metastasis. 17,18 Urokinase-type plasminogen activator (u-PA), a serine proteinase, may initiate the activation of an enzymatic cascade that involves the activation of plasminogen to plasmin, which in turn could cleave and activate MMPs. 19 The activation of these enzymes enables the degradation of extracellular matrix (ECM) by cancer cells, allowing their access to the vasculature, invasion into the target organ and development of tumor metastasis.…”

Section: Introductionmentioning

confidence: 99%

Antimetastatic Potentials of Phyllanthus urinaria L on A549 and Lewis Lung Carcinoma Cells via Repression of Matrix-Degrading Proteases

Tseng¹,

Chen

Kuo

et al. 2011

Integr Cancer Ther

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Tumor metastasis is the most important cause of cancer death and various treatment strategies have targeted at preventing the occurrence of metastasis. Phyllanthus urinaria L is a popular folk medicine and has several proven biological properties, including antioxidant, antihypertension, and anti-inflammatory. This study provides molecular evidence associated with the antimetastatic effects of P urinaria L extracts (PUE), which contained polyphenols including gallic acid, methyl gallate, epicatechin, epigallocatechin-3-gallate, gallocatechin-3-gallate, rutin, epicatechin-3-gallate, and naringin, by showing a marked inhibition on the invasion (P < .001) and migration (P < .001) of highly metastatic A549 and Lewis lung carcinoma (LLC) cells. To further investigate the precise involvement of PUE in tumor metastasis, A549 and LLC cells were treated with PUE at various concentrations and results from zymography and Western blotting showed that a PUE treatment may decrease the expressions of matrix metalloproteinase-2 (MMP-2; P < .001), MMP-9 (P < .001), urokinase plasminogen activator (P < .001), and their endogenous inhibitors, that is, tissue inhibitor of metalloproteinase-2 and plasminogen activator inhibitor-1, in a concentration-dependent manner. Reverse transcription-polymerase chain reaction and MMP-2 promoter luciferase analysis (P < .001) revealed that PUE inhibits the transcription of MMP-2 mRNA. PUE also exerted an inhibitory effect on the DNA-binding activity and the nuclear translocation of NF-κB and AP-1. Furthermore, the inhibitory effects of PUE on the metastasis and growth of LLC cells in vivo were proven. These results indicate that PUE could be applied to be a potential antimetastatic agent.

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Antimetastatic effects of Terminalia catappa L. on oral cancer via a down-regulation of metastasis-associated proteases

Cited by 100 publications

References 37 publications

Cantharidin Impairs Cell Migration and Invasion of Human Lung Cancer NCI-H460 Cells via UPA and MAPK Signaling Pathways

Cantharidin Impairs Cell Migration and Invasion of Human Lung Cancer NCI-H460 Cells via UPA and MAPK Signaling Pathways

Study on synthesis of thalidomide analogues and their bioactivities; inhibition on iNOS pathway and cytotoxic effects

Antimetastatic Potentials of Phyllanthus urinaria L on A549 and Lewis Lung Carcinoma Cells via Repression of Matrix-Degrading Proteases

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