Antilisterial Bacteriocin from Lactobacillus rhamnosus CJNU 0519 Presenting a Narrow Antimicrobial Spectrum

Abstract

A lactic acid bacterium presenting antimicrobial activity against a Lactobacillus acidophilus strain used for eradication of acid inhibition was isolated from a natural cheese. The 16S rRNA gene sequence of the isolate best matched with a strain of L. rhamnosus and was designated L. rhamnosus CJNU 0519. The antimicrobial activity of the partially purified bacteriocin of CJNU 0519 was abolished when treated with a protease, indicating the protein nature of the bacteriocin. The partially purified bacteriocin (rhamnocin 519) displayed a narrow antimicrobial activity against L. acidophilus, Listeria monocytogenes, and Staphylococcus aureus among several tested bacterial and yeast strains. Rhamnocin 519 in particular showed strong bactericidal action against L. monocytogenes.

In silico Prediction, in vitro Antibacterial Spectrum, and Physicochemical Properties of a Putative Bacteriocin Produced by Lactobacillus rhamnosus Strain L156.4

“…lactis, Lactobacillus plantarum , and L. monocytogenes can be attributed to the putative bacteriocin, considering that the mentioned antibacterial spectrum and antilisterial activity are among the main features of class II bacteriocins produced by LAB (Klaenhammer, 1993 ; Drider et al, 2006 ). Our results present some overlapping features with other reports of antimicrobial activity of bacteriocins produced by L. rhamnosus strains against E. faecalis (Todorov and Dicks, 2005 ; Aguilar-Uscanga et al, 2013 ), Micrococcus luteus (Srinivasan et al, 2013 ), S. aureus (Sarika et al, 2010 ; Srinivasan et al, 2013 ; Jeong and Moon, 2015 ), L. monocytogenes (Aguilar-Uscanga et al, 2013 ; Srinivasan et al, 2013 ; Jeong and Moon, 2015 ) and E. coli (Todorov and Dicks, 2005 ). Class II bacteriocins kill bacteria by pore formation or by interfering with the integrity of the target cell membrane, inducing permeabilization and leakage of the intracellular content (Drider et al, 2006 ).…”

“…This species has strain-specific genetic and metabolic characteristics that explain its occurrence in a variety of ecological niches (Douillard et al, 2013 ; Ceapa et al, 2015 ). Bacteriocinogenic L. rhamnosus strains have been isolated from human feces (Gorbach, 1996 ; Gill et al, 2000 ; Cukrowska et al, 2009 ; Dimitrijević et al, 2009 ; Aguilar-Uscanga et al, 2013 ), vaginal microbiota (Li et al, 2005 ), fermented beverages (Todorov and Dicks, 2005 ), grape peels (Sarika et al, 2010 ), milk samples (Srinivasan et al, 2013 ), and cheese (Jeong and Moon, 2015 ). This species is generally recognized as safe (GRAS) and has been widely explored as a probiotic in animal production (Weese and Anderson, 2002 ) human health (Gill et al, 2000 ; Cukrowska et al, 2009 ; Douillard et al, 2013 ; Szajewska and Kołodziej, 2015 ) and as a biopreservative in food systems (Cotter et al, 2005 ; Douillard et al, 2013 ).…”

Biological properties and production of bacteriocins‐like‐inhibitory substances by Lactobacillus sp. strains from human vagina

“…No exception are the seven bacterial species tested in this study (L. fermentum, L. crispatus, L. gasseri, L. jensenii, L. rhamnosus, L. salivarius), some of which are already known for the production of substances with antimicrobial activity (Zhu et al 2000;Kaewsrichan et al 2006;Pascual et al 2008;Dimitrijevic et al 2009;Riaz et al 2010;Nakamura et al 2013;Jeong and Moon 2015).…”

Antilisterial Potential of Lactic Acid Bacteria in Eliminating Listeria monocytogenes in Host and Ready-to-Eat Food Application

“…[29,40,53]. Class IIa bacteriocins such as pediocin PA-1 (Pediococcus acidilactici), enterocin A (Enterococcus faecium), and sakacin A (Lactobacillus sakei), which are classified based on biochemical and genetic properties, consist of a highly conserved hydrophilic N-terminal domain with consensus motif YGNGYV (tyrosine, glycine, asparagine, glycine, tyrosine, valine), known as the pediocin box that is responsible for activity against L. monocytogenes [28,50,[53][54][55]. Their cationic terminal interacts with anionic lipids present in the targeted bacterial cell membrane via electrostatic interaction or specific receptors such as the mannose phosphotransferase (Man-PTS) system on targeted specific bacterial species but not other populations within the same ecosystem [42], ensuring a limited spectrum of inhibition to the targeted bacterial species [39,56].…”