Antilisterial activity of three bacteriocins used at sub minimal inhibitory concentrations and cross-resistance of the survivors

Song, Hyun-Joo; Richard, Jean

doi:10.1016/s0168-1605(97)01254-3

Cited by 52 publications

(35 citation statements)

References 24 publications

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“…However, the IC 50 of nisin for the high-level-resistant mutants was the same as that for the wild type strains, in agreement with other reports (17,46). Cross-resistance between class IIa bacteriocins has been reported frequently (7,17,(46)(47)(48), but reports concerning crossresistance between bacteriocins of different classes contain contradictory results (7,11,41,46,47,52,54).…”

Section: Discussionsupporting

confidence: 88%

“…Various methods are used to determine differences in susceptibility to bacteriocins (6,16,18,38,40,42,47,52,60). To achieve high discrimination between strains with different susceptibilities, a standardized microtiter plate assay, performed with bacteriocin solutions of known concentrations, was used to provide data necessary to determine the potencies of the bacteriocins sakacin P, pediocin PA-1, sakacin A, and nisin against L. monocytogenes.…”

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confidence: 99%

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Differences in Susceptibility of Listeria monocytogenes Strains to Sakacin P, Sakacin A, Pediocin PA-1, and Nisin

Katla

Naterstad²,

Vancanneyt

et al. 2003

Appl Environ Microbiol

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Two hundred strains of Listeria monocytogenes collected from food and the food industry were analyzed for susceptibility to the class IIa bacteriocins sakacin P, sakacin A, and pediocin PA-1 and the class I bacteriocin nisin. The individual 50% inhibitory concentrations (IC 50 ) were determined in a microtiter assay and expressed in nanograms per milliliter. The IC 50 of sakacin P ranged from 0.01 to 0.61 ng ml ؊1 . The corresponding values for pediocin PA-1, sakacin A, and nisin were 0.10 to 7.34, 0.16 to 44.2, and 2.2 to 781 ng ml ؊1 , respectively. The use of a large number of strains and the accuracy of the IC 50 determination revealed patterns not previously described, and for the first time it was shown that the IC 50 of sakacin P divided the L. monocytogenes strains into two distinct groups. Ten strains from each group were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins and amplified fragment length polymorphism. The results from these studies essentially confirmed the grouping based on the IC 50 of sakacin P. A high correlation was found between the IC 50 of sakacin P and that of pediocin PA-1 for the 200 strains. Surprisingly, the correlation between the IC 50 of the two class IIa bacteriocins sakacin A and sakacin P was lower than the correlation between the IC 50 of sakacin A and the class I bacteriocin nisin.

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Section: Discussionsupporting

confidence: 88%

mentioning

confidence: 99%

Differences in Susceptibility of Listeria monocytogenes Strains to Sakacin P, Sakacin A, Pediocin PA-1, and Nisin

Katla

Naterstad²,

Vancanneyt

et al. 2003

Appl Environ Microbiol

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“…faecium was able to reduce the populations of present intestinal microflora in experimental birds, although this effect was especially remarkable after its first oral administration. Similarly, this type of inhibition kinetics has been observed before with Listeria as indicator and nisin, pediocin AcH and enterococcin EFS2 as antimicrobial substances (Song and Richard 1997), and may indicate that sensitive bacteria become resistant to the bacteriocin. It is generally admitted that each bacteriocin-sensitive bacterial population includes potentially tolerant and/or resistant cells with structural modifications or at least with a high predisposition to such modifications, which would allow them to spontaneously emerge in case of exposure to the bacteriocin (Hanlin et al 1993).…”

Section: Resultssupporting

confidence: 74%

Effect of Bacteriocin-like Substance Produced by Enterococcus faecium EF55 on the Composition of Avian Gastrointestinal Microflora

Strompfová¹,

Lauková²,

Mudroňová³

2003

Acta Vet. Brno

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The influence of daily orally administered crude extract of bacteriocin-like substance (at a concentration of 3200 AU/ml) produced by Enterococcus faecium EF55 on the total counts of lactobacilli, staphylococci, enterococci and E. coli in the faeces and caecum of 3-day-old Japanese quails was investigated. Enterococcus faecium EF55 was isolated from the crop content of a chicken (Gallus domesticus). The inhibitory activity of bacteriocin-like substance (BLIS) produced by the strain EF55 was assayed by the agar spot test using Gram-positive and Gramnegative indicator bacteria. A wide range of Gram-positive genera such as Enterococcus, Staphylococcus, Micrococcus, Lactobacillus, Lactococcus, Streptococcus and Aerococcus was susceptible to BLIS, but none of the Gram-negative bacteria. The antimicrobial substance produced by the strain EF55 was thermo-resistant (30 min at 100 °C), stable at pH 4.0 to 9.0 at -20 °C, 4 °C and 22 °C for 10 d tested, and inactivated by proteolytic enzymes indicating its proteinaceous nature. After the first administration of bacteriocin crude extract (BCE) of EF55 strain to Japanese quails, a reduction amounting to 0.83-1.3 log cycles of E. coli, enterococci, staphylococci and lactobacilli in faeces was observed within 24 h. This inhibitory effect was most visible after first extract addition, later this difference was diminished. By agar spot test, BLIS produced by the strain EF55 of Ent. faecium was active against Staphylococcus spp., Lactobacillus spp. and Enterococcus spp., isolates obtained from the experimental birds. However, no inhibition against E. coli was detected, despite of their decreased counts under in vivo conditions. Bacteriocin, Japanese quails, gastrointestinal microflora, effect

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“…Cintas et al (1998) also reported pediocin to be more effective than nisin against some food borne pathogens such as L. monocytogenes. In case of all the bacterial strains it was observed that even when the bacteriocins displayed the most rapid inhibitory activity at 1 h, the survivors resumed growth, reaching the highest cell counts at 24 h. Similar observations were also made by Schillinger et al (1998), who reported a regrowth of survivors of L. monocytogenes Scott A after exposure to nisin concentrations between 10 and 500 IU/ml as well as with those of Song and Richard (1997), who observed that survivors of L. innocua resumed growth after the addition of nisin, pediocin AcH, and enterococcin EFS2 into TSBYE broth. According to Muriana (1996), several studies indicated the immediate decrease of target cells by one to three log cycles cfu/ml when a bacteriocin was added, with none or little effect on future inoculations.…”

Section: Kinetics Of Cell Growth Inhibition By Bacteriocinssupporting

confidence: 79%

Antibacterial efficacy of nisin, pediocin 34 and enterocin FH99 against L. monocytogenes, E. faecium and E. faecalis and bacteriocin cross resistance and antibiotic susceptibility of their bacteriocin resistant variants

et al. 2011

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The bacteriocin susceptibility of Listeria monocytogenes MTCC 657, Enterococcus faecium DSMZ 20477, E. faecium VRE, and E. faecalis ATCC 29212 and their corresponding bacteriocin resistant variants was assessed. The single and combined effect of nisin and pediocin 34 and enterocin FH99 bacteriocins produced by Pediococcus pentosaceus 34, and E. faecium FH99, respectively, was determined. Pediocin34 proved to be more effective in inhibiting L. monocytogenes MTCC 657. A greater antibacterial effect was observed against E. faecium DSMZ 20477 and E. faecium (VRE) when the a combination of nisin, pediocin 34 and enterocin FH99 were used whereas in case of L. monocytogenes MTCC 657 a combination of pediocin 34 and enterocin FH99 was more effective in reducing the survival of pathogen. Bacteriocin cross-resistance and the antibiotic susceptibility of wild type and their corresponding resistant variants were assessed and results showed that resistance to a bacteriocin may extend to other bacteriocins within the same class and also the acquired resistance to bacteriocins can modify the antibiotic susceptibility/resistance profile of the bacterial species used in the study. According to the hydrophobicity nisin resistant variant of L. monocytogenes was more hydrophobic (p<0.001), whereas the pediocin 34 and enterocin FH99 resistant variants were less hydrophobic than the wild type strain. Nisin, pediocin 34 and enterocin FH99 resistant variants of E. faecium DSMZ 20477 and E. faecium VRE were less hydrophobic than their wild type counterparts. Nisin resistant E. faecalis ATCC 29212 was less hydrophobic than its wild type counterpart.

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Antilisterial activity of three bacteriocins used at sub minimal inhibitory concentrations and cross-resistance of the survivors

Cited by 52 publications

References 24 publications

Differences in Susceptibility of Listeria monocytogenes Strains to Sakacin P, Sakacin A, Pediocin PA-1, and Nisin

Differences in Susceptibility of Listeria monocytogenes Strains to Sakacin P, Sakacin A, Pediocin PA-1, and Nisin

Effect of Bacteriocin-like Substance Produced by Enterococcus faecium EF55 on the Composition of Avian Gastrointestinal Microflora

Antibacterial efficacy of nisin, pediocin 34 and enterocin FH99 against L. monocytogenes, E. faecium and E. faecalis and bacteriocin cross resistance and antibiotic susceptibility of their bacteriocin resistant variants

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