Antilipase and antioxidant activity of Orthosiphon stamineus methanolic extract

Abstract

Introduction: Antilipase from natural resources are a potential tool for the treatment of obesity while antioxidant-rich plants are essential in combating degenerative diseases. The aim of this study is to determine the antilipase and antioxidant activity of Orthosiphon stamineus methanolic extract. Methods: The inhibitory activity against pancreatic lipase was determined by measuring the hydrolysis of p-nitrophenyl butyrate to p-nitrophenol at 405 nm. Antioxidant activity of O. stamineus extract was measured by 2, 2, diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity assay. Results: The O. stamineus crude extract exhibited strong lipase inhibitory activity with an IC50 value of 34.7 µg/ml. The inhibition mode study disclosed that O. stamineus could act as uncompetitive inhibitor. O. stamineus showed high antioxidant activity with an EC50 value of 26.3 µg/ml. Conclusions: The results suggest that O. stamineus has shown potential as a source of natural antilipase and antioxidant.

The methanolic extract of Garcinia atroviridis (MeGa) reduces body weight and food intake, and improves lipid profiles by altering the lipid metabolism: a rat model

“…Many herbal products lack scientific evidence on their safety profiles and their respective mechanisms of actions before they are marketed. Despite many studies reporting on the antioxidant and weight reduction properties of the leaves and fruit extracts of GA (Alias et al, 2017;Hamidon et al, 2017;Lumbantobing et al, 2017), no comprehensive study is available on the safety of GA extracts according to standard guidelines such as good laboratory practice and OECD. Therefore, phytochemical composition and safety limits of the methanolic extract of GA (MeGa) fruit were investigated and a metabolomics study was further conducted to study the changes of metabolic pathways in obese rats treated with MeGa.…”