Antileukemic activity of an arsenomolybdate in the human HL-60 and U937 leukemia cells

Li, Chunyan; Cao, Hongqian; Sun, Jiaheng; Tian, Rui; Li, Dongbei; Yang, Wei; Li, Juan

doi:10.1016/j.jinorgbio.2016.12.002

Cited by 17 publications

(6 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MCF-7 cells were grown in 24-well confocal culture dishes and treated with three different concentrations (50, 10 and 5 μM) of V 18 for 24 h at 37 °C, 5% CO 2 . After that, the apoptotic cells were detected by staining with Hoechst 33342 and PI [ 25 ]. The cells were observed by fluorescence confocal microscopy (Olympus FV1000, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

The Anti-Proliferation Activity and Mechanism of Action of K12[V18O42(H2O)]∙6H2O on Breast Cancer Cell Lines

Zhang

Guo

et al. 2017

Molecules

Self Cite

View full text Add to dashboard Cite

Polyoxometalates (POMs) are inorganic clusters that possess potential anti-bacterial, anti-viral, and anti-tumor activities. Herein, the in vitro anti-proliferation activities of K12[V18O42(H2O)]∙6H2O (V18) have been investigated on the MCF-7 and MDA-MB-231 cell lines. The results indicated that V18 could inhibit the proliferation of MCF-7 (IC50, 11.95 μM at 48 h) in a dose-dependent manner compared to the positive control, 5-fluorouracil (5-Fu, p < 0.05). The anti-proliferation activity of V18 might be mediated by arrest of the MCF-7 cells in the G2/M phase and induction of apoptosis and necrosis. Moreover, V18 can effectively quench the fluorescence of ctDNA. The binding mode between them may be groove or outside stacking binding. V18 can also effectively quench the intrinsic fluorescence of bovine serum albumin (BSA) and human serum albumin (HSA) via static quenching, and changed the conformation of BSA and HSA.

show abstract

Section: Methodsmentioning

confidence: 99%

The Anti-Proliferation Activity and Mechanism of Action of K12[V18O42(H2O)]∙6H2O on Breast Cancer Cell Lines

Zhang

Guo

et al. 2017

Molecules

Self Cite

View full text Add to dashboard Cite

show abstract

“…The K 2 Na[AsMo 6 O 21 (O 2 CCH 2 NH 3 ) 3 ]·6H 2 O (POM), was prepared according to the previously described approach [40]. RPMI-1640 Medium and Fetal bovine serum (FBS) were purchased from Hyclone, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), phosphate buffer saline (PBS) and HSA were obtained from Sigma-Aldrich.…”

Section: Methodsmentioning

confidence: 99%

“…And in the study of anti-leukemia, the compound showed low inhibitory effect on normal human umbilical vein endothelial cells (HUVEC) with an IC 50 value of 889.18 μM and a significant anticancer effect on human HL-60 and U937 leukemia cells [40]. Herein, we aim to evaluate the cytotoxicity of pure POMs and nanoparticles on MCF-7 cell line.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, cytotoxicity and antitumour mechanism investigations of polyoxometalate doped silica nanospheres on breast cancer MCF-7 cells

Cao

et al. 2017

PLoS ONE

Self Cite

View full text Add to dashboard Cite

Polyoxometalates (POMs) have shown the potential anti-bacterial, anti-viral and anti-tumor activities. In order to improve their physiological stability and antitumour activity for medical application, K2Na[AsIIIMo6O21(O2CCH2NH3)3]·6H2O doped silica nanospheres (POM@SiO2) with diameters of ~40 nm have been synthesized by the water-in-oil microemulsion method in this study. The obtained spheres were morphologically uniform nanosized and nearly monodispersed in solution. The nanoparticles had high entrapment efficiency, which was upto 46.2% by the inductively coupled plasma mass spectrometry (ICP-MS) analysis and POMs slowly released from the nanospheres both in the PH 7.4 and 5.5 phosphate buffer saline (PBS) solutions in 60 h. The in vitro MTT assays of particles on MCF-7 cell line (a human breast adenocarcinoma cell line) exhibited enhanced antitumor activity compared to that of plain polyoxometalate. The IC50 value of the POM@SiO2 nanoparticles was 40.0 μg/mL at 24 h calculated by the encapsulated POM concentration, which was much lower comparing to that of 2.0 × 104 μg/mL according to the pure POM. And the SiO2 shells showed low inhibitory effect at the corresponding concentration. Confocal images further indicated the cell morphology changes and necrosis. Flow cytometric analysis showed nanoparticles induced the apoptosis by arresting the cells in S phase and western blot analysis indicated they promoted apoptosis by inhibiting the Bcl-2 protein. Moreover, the study of interactions between human serum albumin (HSA) and the nanoparticles indicated the fluorescence quenching was static, and the nanoparticles were likely to bind to HSA and changed its conformation.

show abstract

“…Li et al 8 developed an arsenopolybdate (K 2 Na[AsMo 6 O 21 (O 2 CCH 2 NH 3 ) 3 ]·6H 2 O), evaluated it by human leukemia cells (HL-60 and U937), and showed antileukemia activity. The nuclear morphology analysis showed that with the increase in the compound dose, the nucleus decreased and the number of dead cells increased.…”

Section: Application Of Polyoxometalates In Anticancer Therapymentioning

confidence: 99%

Polyoxometalate-based nanocomposites for antitumor and antibacterial applications

Chang

Chen

et al. 2022

Nanoscale Adv.

View full text Add to dashboard Cite

show abstract

Antileukemic activity of an arsenomolybdate in the human HL-60 and U937 leukemia cells

Cited by 17 publications

References 45 publications

The Anti-Proliferation Activity and Mechanism of Action of K12[V18O42(H2O)]∙6H2O on Breast Cancer Cell Lines

The Anti-Proliferation Activity and Mechanism of Action of K12[V18O42(H2O)]∙6H2O on Breast Cancer Cell Lines

Synthesis, cytotoxicity and antitumour mechanism investigations of polyoxometalate doped silica nanospheres on breast cancer MCF-7 cells

Polyoxometalate-based nanocomposites for antitumor and antibacterial applications

Contact Info

Product

Resources

About