Antileukaemic Cell Proliferation and Cytotoxic Activity of Edible Golden Cordyceps (Cordyceps militaris) Extracts

Abstract

Golden cordyceps (Cordyceps militaris) is a mushroom of the genus Cordyceps. It has been used as a food supplement for both healthy and ill people. In this study, the antileukaemic cell proliferation activities of golden cordyceps extracts were examined and compared with standard cordycepin (CDCP) in EoL-1, U937, and KG-1a cells. Wilms’ tumour 1 (WT1) protein was used as a biomarker of leukaemic cell proliferation. The cytotoxicity of the extracts on leukaemic cells was determined using the MTT assay. Their inhibitory effects on WT1 protein expression and cell cycle progression of EoL-1 cells were investigated using Western blotting and flow cytometry, respectively. Induction of KG-1a cell differentiation (using CD11b as a marker) was determined using flow cytometry. The golden cordyceps extracts exhibited cytotoxic effects on leukaemic cells with the highest IC50 value of 16.5 ± 3.9 µg/mL, while there was no effect on normal blood cells. The expression levels of WT1 protein in EoL-1 cells were decreased after treatment with the extracts. Moreover, cell cycle progression and cell proliferation were inhibited. The levels of CD11b increased slightly following the treatment. All these findings confirm the antileukaemic proliferation activity of golden cordyceps.

The regulation of ISG20 expression on SARS-CoV-2 infection in cancer patients and healthy individuals

“…We, therefore, further tested whether CD and m 6 2 A could affect ISG20 expression and found that both promoted ISG20 expression at the protein and mRNA levels. CD is a natural active component of traditional Chinese medicine (TCM) fungus cordyceps militaris, which has anticancer properties (33)(34)(35). m 6 2 A is a modified ribonucleoside in the tRNA of mycobacterium bovis, according to Bacille Calmette-Gueŕin (36).…”

Antimicrobial Properties Related to Anti-Acne and Deodorant Efficacy of Hedychium coronarium J. Koenig Extracts from Pulsed Electric Field Extraction

“…The resulting extracts were filtered through Whatman No. 1 filter paper (Merck KGaA, Darmstadt, Germany) and the solvent pooled from three cycles of maceration was evaporated by a rotary evaporator (Buchi Evaporator, Heidolph, Germany) [43]. The extract was obtained and stored at 4 • C until the next usage.…”

Anti-Cancer Potential of Edible/Medicinal Mushrooms in Breast Cancer

“…Several active compounds are present in Cordyceps mushrooms, such as ergosterol, mannitol, and modified nucleosides [ 69 ], but the major functional component with medicinal properties shared by both the aforementioned fungi is cordycepin, whose anticancer effects on various tumors, including BC, have been reported [ 63 ]. These antitumoral properties are attributed to structural similarity with cellular nucleosides and adenosine: cordycepin, lacking oxygen at the 3′-position of its ribose moiety, acts like a nucleoside analog and inhibits the polyadenylation of the mRNA of cancer cells [ 65 ].…”