Antileishmanial, antitrypanosomal, and cytotoxic screening of ethnopharmacologically selected Peruvian plants

González-Coloma⊥, Azucena; Reina, Matı́as; Sáenz, Claudia; Lacret, Rodney; Mesia, Lastenia Ruiz; Arán, Vicente J.; Sanz, Jesus; Martínez-Díaz, Rafael A.

doi:10.1007/s00436-011-2638-3

Cited by 71 publications

(64 citation statements)

References 44 publications

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“…N nucleus, Fl flagella, ER endoplasmic reticulum revealed the presence of saponin, steroids, tannins, glycosides, alkaloids and flavonoids (Issazadeh et al 2012). In general, isolated plant's substances such as flavonoids, alkaloids, cumarins, saponins and glycosides have demonstrated activity against different flagellates such as G. lamblia (Barbosa et al 2007), Trichomonas gallinae (Adebajo et al 2006), T. vaginalis (Arthan et al 2008), and Leishmania infantum (González-Coloma et al 2011). Anti-T. vaginalis activity of other plant's saponins was reported (Rocha et al 2012).…”

Section: Discussionmentioning

confidence: 99%

In vitro anti-Trichomonas vaginalis activity of Pistacia lentiscus mastic and Ocimum basilicum essential oil

Eldin

Badawy

2013

J Parasit Dis

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Trichomonas vaginalis is a protozoan parasite that causes trichomoniasis; a cosmopolitan sexually transmitted disease. Metronidazole is the drug of choice for T. vaginalis infections. The increase in metronidazole resistant parasites and undesirable side effects of this drug makes the search for an alternative a priority for the management of trichomoniasis. Pistacia lentiscus mastic and Ocimum basilicum oil are known for their antibacterial, antifungal, antiviral and antiprotozoal effects. The present study was carried out to investigate the in vitro effects of P. lentiscus mastic and O. basilicum oil on T. vaginalis trophozoites. The effects of different concentrations of P. lentiscus mastic (15, 10 and 5 mg/ml) and different concentrations of O. basilicum oil (30, 20 and 10 lg/ml) on multiplication of trophozoites at different time points (after 24, 48, 72 and 96 h) were determined, also morphological changes were reported by transmission electron microscopy (TEM). The results showed that both plants caused an inhibition of growth of T. vaginalis trophozoites. The minimal lethal concentration of P. lentiscus mastic was 15 mg/ml after 24 h incubation, 10 mg/ml after 48 h and 5 mg/ml after 96 h. The minimal lethal concentration of O. basilicum oil was 30 lg/ml after 24 h incubation, 20 lg/ml after 48 h and 10 lg/ml after 96 h. TEM study of trophozoites treated by P. lentiscus mastic or by O. basilicum oil showed considerable damage of the membrane system of the trophozoites, and extensive vacuolization of the cytoplasm. These results highly suggest that P. lentiscus mastic and O. basilicum oil may be promising phytotherapeutic agents for trichomoniasis treatment.

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Section: Discussionmentioning

confidence: 99%

In vitro anti-Trichomonas vaginalis activity of Pistacia lentiscus mastic and Ocimum basilicum essential oil

Eldin

Badawy

2013

J Parasit Dis

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“…A pesar de que H. grandella ha sido estudiada (Cornelius & Watt, 2003;Hilje, & Cornelius, 2001;Pérez-Salicrup, & Esquivel, 2008;Soto, Hilje, Mora, & Carballo, 2011), aún no se ha desarrollado un sistema de manejo integrado. Algunos metabolitos secundarios de aceites y extractos de C. odorata han sido evaluados con diferente finalidad, entre ellas, la búsqueda de nuevos limonoides antialimentarios, la implicación ecológica en la atracción de H. grandella, evaluación de las propiedades inhibitorias de crecimiento frente algunos insectos y la investigación de sus propiedades medicinales (Asekun, & Ekundayo, 1999;Da Silva et al, 1999;De Paula et al, 1997;González-Coloma et al, 2012;Maia et al, 2000;Kipassa et al, 2008;Martins et al, 2003;Omar et al, 2003;Silva, Souza, Rogez, Rees, & Larondelle, 2007;Villanueva, Tuten, Haber, & Setzer, 2009). Sin embargo, no se han evaluado las posibles diferencias cuantitativas y cualitativas de diferentes individuos de C. odorata.…”

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Evaluación de fenoles y limonoides en hojas de Cedrela odorata (Meliaceae) de una plantación experimental establecida en Tezonapa Veracruz, México

Mariscal-Lucero

Rosales-Castro

Sánchez-Monsalvo

et al. 2014

RBT

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Cedrela odorata (Meliaceae) is a native timber tree to Tropical America, known for its high-quality wood, unfortunately, plantations of this species are severely attacked by Hypsipyla grandella. The attraction or repellency of this pest is related to secondary metabolites such as phenols and limonoids (triterpenes); therefore, it is important to study these compounds to understand the phytochemical phenomena behind this problem. With this aim, the concentration of total phenols and limonoides was evaluated in C. odorata leaves from a plantation established in Tezonapa Veracruz, Mexico. For this, a total of 66 tree leaves samples, from seven sites, were analyzed. Phenols and limonoids concentration showed significant differences not only among different provenances, but also among individual trees of the same site (Tukey, p≤0.05). Phenols concentration was variable and in the range from 49 to 223mg EAG/g e for total phenols, from 7 to 158mg EC/g e for flavonoids and from 4 to 104mg EC/g e for proanthocyanidins. Limonoids concentration was also variable, ranging between 227 and 748mg EL/g e. A major compound was found by High-Performance Liquid Chromatography with Ultraviolet Diode Array Detection (HPLC-UV-DAD), which corresponded to a flavonol kaempferol glycoside derivative; additionally, a flavanol catechin was also detected at low concentrations. GC–MS allowed the identification of the sesquiterpenoids β-elemene, E-caryophyllene, aromadendrene, α-humulene, γ-cadinene, D-germacrene, bicyclogermacrene, and the poly terpenoids D-α-tocopherol and β-sitosterol. Our results suggest that the evaluation of phenols may play an important role as a selection parameter for improvement and conservation programs, if they are complemented with conventional breeding practices.

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“…Plants produce a high diversity of low molecular weight compounds and in recent years some of these phytocompounds have also shown good antiparasitic effects in laboratory trials (Anthony et al 2005;Kumar et al 2012;Nagajyothi et al 2012;Teichmann et al 2012;Zahir et al 2012;González-Coloma et al 2012;Koné et al 2012). The Northeast region of India is blessed with a rich wealth of medicinal plants, whose decoctions, infusions, syrups, etc.…”

Section: Introductionmentioning

confidence: 99%

In vitro anthelmintic assessment of selected phytochemicals against Hymenolepis diminuta, a zoonotic tapeworm

Vijaya

Yadav

2014

J Parasit Dis

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A large number of medicinal plants are used as herbal remedy for the treatment of helminthic diseases in the developing countries, however, far too little attention has been paid to assess the anthelmintic potentials of chemical compounds that are present in these plants. This study was carried out to assess the in vitro anthelmintic effects of biochanin A, ursolic acid, betulinic acid and betasitosterol which are the major phytochemicals of Trifolium repens, Houttuynia cordata and Lasia spinosa, the traditionally used anthelmintic plants of Northeast India. The in vitro anthelmintic testing of these phytochemicals was undertaken against Hymenolepis diminuta, a zoonotic tapeworm, and their efficacy was compared with a reference drug, praziquantel. The results revealed that except beta-sitosterol, which showed a very weak anthelmintic effect, remaining all other tested compounds possess highly significant (p B 0.001) and dose-dependent anthelmintic effects. Upon exposure to 0.25, 0.50 and 1 mg/ml concentrations of biochanin A, ursolic acid and betulinic acid, the test parasite H. diminuta, at first, showed a paralyzed state which later culminated into their mortality after short time periods. Of all the phytochemicals tested, betulinic acid (1 mg/ml) showed the best anthelmintic effect and caused the mortality of test parasites at 3.4 ± 0.66 h. In conclusion, the results of this study demonstrate for the first time that betulinic acid, biochanin A and ursolic acid possess significant in vitro anthelmintic effects against H. diminuta, a zoonotic tapeworm, and, therefore, these compounds may be exploited further for anthelmintic drug development.

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Antileishmanial, antitrypanosomal, and cytotoxic screening of ethnopharmacologically selected Peruvian plants

Cited by 71 publications

References 44 publications

In vitro anti-Trichomonas vaginalis activity of Pistacia lentiscus mastic and Ocimum basilicum essential oil

In vitro anti-Trichomonas vaginalis activity of Pistacia lentiscus mastic and Ocimum basilicum essential oil

Evaluación de fenoles y limonoides en hojas de Cedrela odorata (Meliaceae) de una plantación experimental establecida en Tezonapa Veracruz, México

In vitro anthelmintic assessment of selected phytochemicals against Hymenolepis diminuta, a zoonotic tapeworm

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