Antileishmanial activity and cytotoxicity of Brazilian plants

Ribeiro, Tatiana G.; Chávez-Fumagalli, Miguel Á.; Valadares, Diogo G.; Franca, Juçara Ribeiro; Lage, Paula S.; Duarte, Mariana C.; Andrade, Pedro H.R.; Martins, Vívian T.; Costa, Lourena E.; Arruda, Ana Lúcia Alves de; Faraco, André Augusto Gomes; Coelho, Eduardo Antônio Ferraz; Castilho, Rachel Oliveira

doi:10.1016/j.exppara.2014.05.004

Cited by 60 publications

(37 citation statements)

References 69 publications

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“…Nevertheless, it is a preliminary cell-based assay with 24 h exposure of cells to the test substances, which would be rather different from the clinical use. Ribeiro et al (2014) reported that S. cumini hexanic extract has low toxicity on murine macrophages, with IC 50 of 31.64 lg/mL. There have been no reports on the toxicity of S. obtusifolium in vitro.…”

Section: Discussionmentioning

confidence: 99%

Antifungal potential ofSideroxylon obtusifoliumandSyzygium cuminiand their mode of action againstCandida albicans

Pereira

Freires

Castilho

et al. 2016

Pharmaceutical Biology

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Context The emergence of resistant pathogens and toxicity of antifungals have encouraged an active search for novel candidates to manage Candida biofilms. Objective In this study, the little known species Sideroxylon obtusifolium T.D. Penn (Sapotacea) and Syzygium cumini (L.) Skeels (Myrtaceae), from the Caatinga biome in Brazil were chemically characterized and explored for their antifungal potential against C. albicans. Materials and methods We determined the effects of hydroalcoholic extracts/fractions upon fungal growth (minimum inhibitory and fungicidal concentrations, MIC/MFC), biofilm morphology (scanning electron microscopy) and viability (confocal laser scanning microscopy), proposed their mode of action (sorbitol and ergosterol assays), and finally investigated their effects against macrophage and keratinocyte cells in a cell-based assay. Data were analysed using one-way analysis of variance with Tukey-Kramer post-test (a ¼ 0.05). Results The n-butanol (Nb) fraction from S. obtusifolium and S. cumini extract (Sc) showed flavonoids (39.11 6 6.62 mg/g) and saponins (820.35 6 225.38 mg/g), respectively, in their chemical composition and demonstrated antifungal activity, with MICs of 62.5 and 125 lg/mL, respectively. Nb and Sc may complex with ergosterol as there was a 4-16-fold increase in MICs in the presence of exogenous ergosterol, leading to disrupted permeability of cell membrane. Deleterious effects were observed on morphology and viability of treated biofilms from concentrations as low as their MICs and higher. Sc was not toxic to macrophages and keratinocytes at these concentrations (p > 0.05), unlike Nb. Conclusions Nb and Sc demonstrated considerable antifungal activity and should be further investigated as potential alternative candidates to treat Candida biofilms. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Antifungal potential ofSideroxylon obtusifoliumandSyzygium cuminiand their mode of action againstCandida albicans

Pereira

Freires

Castilho

et al. 2016

Pharmaceutical Biology

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“…Contudo para extratos hexânico e metanólico de folhas E. uniflora não se verificou atividade promissora contra formas promastigotas de L. amazonensis, apresentando valores de IC 50 maiores que 200 μg/mL e 250 μg/ mL, respectivamente (BRAGA et al, 2007;RIBEIRO et al, 2014).…”

Section: Psidium Salutareunclassified

Avaliação Da Atividade Leishmanicida in Vitro De Espécies Da Família Myrtaceae, Nativas Do Sul Do Brasil

Kauffmann

Pacheco

Buhl

et al. 2017

Destaques Acadêmicos

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“…Extratos hexânico e metanólico de folhas Eugenia uniflora, conhecida popularmente como pitanga, não demonstraram atividade promissora contra formas promastigotas de L. amazonensis, apresentando valores de IC 50 maiores que 200 µg/mL e 250 µg/ mL, respectivamente (BRAGA et al, 2007;RIBEIRO et al, 2014). No entanto, o óleo essencial de folhas da espécie apresentou atividade leishmanicida in vitro contra promastigotas (IC 50 =3,04 g/mL) e amastigotas (IC 50 = 1,92 g/mL) de L. amazonensis, além de toxicidade preferencial para as formas amastigotas comparada aos macrófagos.…”

Section: Plantas Como Fonte Para O Desenvolvimento De Medicamentos: Ounclassified

“…Os extratos hidroetanólico, metanólico e hexânico de folhas da espécie demonstraram atividade leishmanicida in vitro contra formas promastigotas de L. amazonensis com valores de IC 50 de 166,6 µg/mL, superior a 250 µg/mL e de 31,64 ± 0.99 µg/mL, respectivamente (BEZERRA et al, 2006;BRAGA et al, 2007;RIBEIRO et al, 2014). O óleo essencial das folhas de E. jambolana teve sua constituição determinada, sendo identificados α-pineno (31,85%), (Z)-β-ocimeno (28,98%) e (E)-β-ocimeno (11,71%) como os principais componentes.…”

Section: Plantas Como Fonte Para O Desenvolvimento De Medicamentos: Ounclassified

Potencialidade De Espécies Da Família Myrtaceae Como Fonte Para Obtenção De Novos Candidatos a Fármacos Para O Tratamento De Leishmanioses

Kauffmann

Ethur

2016

Cad. Pedagógico

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Resumo: Leishmanioses são doenças infecciosas causadas por leishmanias e transmitidas por vetores, classificadas como uma doença tropical negligenciada, as quais afetam principalmente populações pobres em países em desenvolvimento. O tratamento das leishmanioses é deficitário em eficácia e segurança, não há vacinas disponíveis e ocorre dificuldade no controle de vetores, o que impulsiona a busca por novas alternativas terapêuticas, entre as quais, plantas. Assim, este artigo apresenta a problemática das leishmanioses e estudos sobre atividade leishmanicida realizados com espécies pertencentes à família Myrtaceae.Palavras-chave: Doenças tropicais negligenciadas. Leishmanioses. Myrtaceae. Plantas Medicinais. POTENTIALITY OF SPECIES OF THE FAMILY MYRTACEAE AS SOURCE FOR OBTAINING NEW CANDIDATES FOR DRUGS FOR THE TREATMENT OF LEISHMANIASISAbstract: Leishmaniasis are infectious diseases caused by leishmania and transmitted by vectors, classified as a neglected tropical disease, which mainly affect poor people in developing countries. Treatment of leishmaniasis is deficient on efficacy and safety, there is no vaccine available and the control of vectors is difficult, which drives the search for new therapeutic alternatives, including plants. Thus, this article presents the problem of leishmaniasis and antileishmanial activity studies performed with species belonging to the Myrtaceae family.

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Antileishmanial activity and cytotoxicity of Brazilian plants

Cited by 60 publications

References 69 publications

Antifungal potential ofSideroxylon obtusifoliumandSyzygium cuminiand their mode of action againstCandida albicans

Antifungal potential ofSideroxylon obtusifoliumandSyzygium cuminiand their mode of action againstCandida albicans

Avaliação Da Atividade Leishmanicida in Vitro De Espécies Da Família Myrtaceae, Nativas Do Sul Do Brasil

Potencialidade De Espécies Da Família Myrtaceae Como Fonte Para Obtenção De Novos Candidatos a Fármacos Para O Tratamento De Leishmanioses

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